Isolation and characterization of Leishmania infantum cDNA encoding a protein homologous to eukaryotic elongation factor 1 gamma.

Abstract:

:This paper reports the isolation and characterization of a complementary deoxyribonucleic acid clone showing sequence homology with genes coding for the eukaryotic elongation factor 1 gamma (EF-1 gamma). The clone encodes an open reading frame of 404 amino acids corresponding to a deduced molecular mass of 46.2 kDa. Database searches revealed 30-64% sequence identity between the Leishmania infantum EF-1 gamma and several eukaryotic homologues. Southern blot analysis indicated that 2 genes tandemly organized were present in the L. infantum genome. The 3' untranslated regions of these 2 genes differed in size. Southern hybridization and pulsed field gel electrophoresis showed that EF-1 gamma genes are highly conserved among members of the Leishmania genus and must be clustered in a single chromosomal locus.

authors

Cañavate C,el Bali M,Montoya Y,Barker DC,Alvar J

doi

10.1016/s0035-9203(02)90050-7

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

S41-7

eissn

0035-9203

issn

1878-3503

journal_volume

96 Suppl 1

pub_type

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