当前位置： SCI文献检索 > TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE期刊下所有文献 > An efficient PCR--SSCP-based method for detection of a chloroquine resistance marker in the PfCRT gene of Plasmodium falciparum.

An efficient PCR--SSCP-based method for detection of a chloroquine resistance marker in the PfCRT gene of Plasmodium falciparum.

Abstract：

:The spread of chloroquine resistance throughout the world poses a major problem in combating malaria. In the present study, an efficient polymerase chain reaction-single strand conformational polymorphism (PCR--SSCP)-based assay detected the PfCRT K76T point mutation, which is a marker for chloroquine resistance. For the first time, we have used a PCR--SSCP-based technique to identify the mutation in a single-step labelling reaction during PCR and SSCP gel electrophoresis. This assay is 100% efficient, giving no false-positive or -negative results, and can be carried out within a short bench time. We have successfully analysed 120 natural isolates using the PCR-SSCP method for detection of the chloroquine resistance marker and found 91 of the 120 samples to show the PfCRT T76 mutation, and 71% (65 of the 91 samples) showed a positive correlation with chloroquine resistance from the clinical data of the patients. The PCR-SSCP technique can also be applied for the detection of new haplotypes of the PfCRT gene and surveillance of chloroquine-resistant malaria in malaria-endemic localities around the world.

journal_name

Trans R Soc Trop Med Hyg

journal_title

Transactions of the Royal Society of Tropical Medicine and Hygiene

authors

Mishra S,Raj DK,Hazra RK,Dash AP,Supakar PC

doi

10.1016/j.trstmh.2005.05.020

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

243-7

issue

3

eissn

0035-9203

issn

1878-3503

pii

S0035-9203(05)00278-6

journal_volume

100

pub_type

杂志文章

相关文献

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE文献大全
  • Detection of Giardia duodenalis antigen in coprolites using a commercially available enzyme-linked immunosorbent assay.

    abstract：:The objective of this experiment was to assess the utility of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for diagnosis of giardiasis in archaeological human remains. The kit, a monoclonal antibody assay, is used to detect the presence of Giardia-specific antigen 65 (GSA65) in human faeces. ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,多中心研究

    doi：10.1016/s0035-9203(02)90337-8

    authors： Gonçalves ML,Araújo A,Duarte R,da Silva JP,Reinhard K,Bouchet F,Ferreira LF

    更新日期：2002-11-01 00:00:00

  • Characterisation of DDT, pyrethroid and carbamate resistance in Anopheles funestus from Obuasi, Ghana.

    abstract：:Indoor-resting anopheline mosquitoes were collected from Obuasi, Ghana, and were identified morphologically and by PCR as Anopheles funestus Giles. Wild-caught females were induced to lay eggs. Samples of F1 progeny from each family were divided into cohorts and were either exposed to DDT and permethrin or were stored...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2008.02.022

    authors： Okoye PN,Brooke BD,Koekemoer LL,Hunt RH,Coetzee M

    更新日期：2008-06-01 00:00:00

  • Clinical characteristics of laboratory-confirmed leptospirosis in Okinawa, Japan, 1974-2015: high incidence of Jarisch-Herxheimer reaction.

    abstract：BACKGROUND:Leptospirosis is a zoonotic disease known to have wide-ranging clinical manifestations. Despite a number of published case series, culture-confirmed series are few and there is a paucity of data on Jarisch-Herxheimer reaction (JHR) associated with treatment of leptospirosis. Our objective was to describe the...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/trw061

    authors： Tsuha S,Taniguchi T,Shiiki S,Narita M,Leung DT

    更新日期：2016-09-01 00:00:00

  • Interstitial pneumonitis in human visceral leishmaniasis.

    abstract：:The involvement of the lung in 13 cases of human visceral leishmaniasis was studied. Interstitial pneumonitis with mononuclear cells was found in 76.8% of the cases; 53.8% also had foci of septal fibrosis. Leishmania were seen within macrophages in 3 cases only. However, all 10 interstitial pneumonitis cases showed PA...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(89)90712-8

    authors： Duarte MI,da Matta VL,Corbett CE,Laurenti MD,Chebabo R,Goto H

    更新日期：1989-01-01 00:00:00

  • Exercise electrocardiogram tests in manual workers with and without antibodies to Trypanosoma cruzi: a population-based study.

    abstract：:Blood pressure, heart rate and electrocardiographic responses to exercise were compared in a group of 125 subjects with antibodies to Trypanosoma cruzi and in 153 seronegative subjects, randomly selected from persons with no evidence of heart disease in a resting electrocardiogram during a cross-sectional survey among...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(90)90081-o

    authors： Zicker F,Zicker EM,Oliveira JJ,Netto JC,Oliveira RM,Smith PG

    更新日期：1990-11-01 00:00:00

  • Molecular diversity of Mycobacterium tuberculosis isolates from patients with pulmonary tuberculosis in Sri Lanka.

    abstract：:The strain diversity of 100 Mycobacterium tuberculosis isolates collected over a period of 18 months from tuberculosis (TB) cases in Sri Lanka was studied by spoligotyping. When compared to the international spoligotyping database, 43 spoligotype patterns were identified, of which 20 were previously described. The maj...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2008.04.025

    authors： Rajapaksa US,Victor TC,Perera AJ,Warren RM,Senevirathne SM

    更新日期：2008-10-01 00:00:00

  • Neurotoxic envenoming by South American coral snake (Micrurus lemniscatus helleri): case report from eastern Ecuador and review.

    abstract：:A man bitten by a large coral snake (Micrurus lemniscatus helleri) in the Amazon basin of Ecuador developed persistent excruciating pain in the bitten arm. On admission to hospital less than 30 min later, he had a polymorphonuclear leucocytosis, thrombocytopenia and mildly prolonged prothrombin time/partial thrombopla...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1016/j.trstmh.2008.03.026

    authors： Manock SR,Suarez G,Graham D,Avila-Aguero ML,Warrell DA

    更新日期：2008-11-01 00:00:00

  • Geospatial analysis of dengue emergence in rural areas in the Southern Province of Sri Lanka.

    abstract：BACKGROUND:Dengue is a major cause of acute febrile illness in Sri Lanka. Dengue has historically been considered an urban disease. In 2012-2013, we documented that acute dengue was surprisingly associated with self-reported rural residence in the Southern Province of Sri Lanka. METHODS:Patients admitted with an acute...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/trz123

    authors： Mutucumarana CP,Bodinayake CK,Nagahawatte A,Devasiri V,Kurukulasooriya R,Anuradha T,De Silva AD,Janko MM,Østbye T,Gubler DJ,Woods CW,Reller ME,Tillekeratne LG,Lantos PM

    更新日期：2020-06-01 00:00:00

  • Amodiaquine fails to cure chloroquine resistant Plasmodium falciparum in the Punjab.

    abstract：:We evaluated amodiaquine as a replacement drug for treating falciparum malaria in an area of Pakistani Punjab where chloroquine-resistant Plasmodium falciparum has recently emerged. Amodiaquine appeared to be 4 to 8 times more effective than chloroquine when P. falciparum isolates were tested in vitro. However, the re...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(87)90311-7

    authors： Khaliq AA,Fox E,Sarwar M,Strickland GT

    更新日期：1987-01-01 00:00:00

  • Sero-molecular evaluation of Toxoplasma gondii infection among HIV-positive patients.

    abstract：BACKGROUND:Toxoplasmosis is one of the most common comorbidities in HIV-positive patients with CD4+ T lymphocytes below 200 cells/μl. Early diagnosis and treatment of toxoplasmosis reduces the mortality rate in HIV-positive people. The aim of this study was to estimate the seroprevalence of Toxoplasma gondii infection ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/trz082

    authors： Ahmadpour E,Pishkarie-Asl R,Spotin A,Samadi Kafil H,Didarlu H,Azadi Y,Barac A

    更新日期：2019-12-01 00:00:00

  • Spatial and temporal variation in biting rates and parasite transmission potentials of onchocerciasis vectors in Ecuador.

    abstract：:The influence of spatial and temporal factors on onchocerciasis transmission by Simulium exiguum s.l. and S. quadrivittatum in Ecuador was investigated to help develop sampling protocols for entomological surveillance of ivermectin programmes. Flies were collected in alternate months (November 1995-November 1996) at f...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2004.03.012

    authors： Vieira JC,Brackenboro L,Porter CH,Basáñez MG,Collins RC

    更新日期：2005-03-01 00:00:00

  • pH of amoebic liver pus.

    abstract：:Amoebic liver pus has an acidic pH. Hence the pleural aspirate from an amoebic empyema due to rupture of an amoebic liver abscess has an acidic pH. In contrast, the pH of pleural aspirates from effusions and empyema of non-amoebic aetiology were noted to have an alkaline pH. The clinical significance of these observat...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 临床试验,杂志文章

    doi：10.1016/0035-9203(76)90180-2

    authors： Ramachandran S,Induruwa PA,Perera MV

    更新日期：1976-01-01 00:00:00

  • Changes in ocular onchocerciasis after two rounds of community-based ivermectin treatment in a holo-endemic onchocerciasis focus.

    abstract：:In a longitudinal study to determine the effect of annual community-based treatment of ocular onchocerciasis with ivermectin, the population living in the 3 most affected villages in the holo-endemic onchocerciasis focus of Asubende in Ghana were re-examined 16 and 24 months after initiating treatment. Ocular microfil...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(91)90051-y

    authors： Dadzie KY,Remme J,De Sole G

    更新日期：1991-03-01 00:00:00

  • Serological survey of Prospect Hill virus infection in indigenous wild rodents in the USA.

    abstract：:We found serological evidence of infection with Prospect Hill virus, a Hantaan-like virus isolated from meadow voles (Microtus pennsylvanicus), in microtine and cricetid rodents trapped in Maryland, West Virginia, Minnesota and California, USA. Fluorescent antibodies were detected in sera from M. pennsylvanicus (74/27...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(87)90275-6

    authors： Yanagihara R,Daum CA,Lee PW,Baek LJ,Amyx HL,Gajdusek DC,Gibbs CJ Jr

    更新日期：1987-01-01 00:00:00

  • Prevalence and clinical presentation of glucose-6-phosphate dehydrogenase deficiency in Pakistani Pathan and Afghan refugee communities in Pakistan; implications for the use of primaquine in regional malaria control programmes.

    abstract：:Glucose-6-phosphate dehydrogenase (G-6-PD) deficiency surveys in Afghan refugees and a local community in the North-West Frontier Province, Pakistan, showed that this trait was most common among Pathan and Uzbak refugees (15.8% and 9.1% respectively). The prevalence among Pakistani Pathans was 7.0%, and that in Tajik ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(95)90661-4

    authors： Bouma MJ,Goris M,Akhtar T,Khan N,Khan N,Kita E

    更新日期：1995-01-01 00:00:00

  • Community-based research: cost of the tests used for anti-HBc total seropositivity only and hepatitis B screening.

    abstract：:Our study aimed to determine anti-HBc total (IgG+IgM) seroprevalence in the adult population aged ≥ 15 and to compare the cost of testing for HBsAg and anti-HBs in only anti-HBc positive+ subpopulation to that in the whole population for HBV screening. The study involved a face-to-face survey and peripheral blood samp...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2010.08.012

    authors： Ozkan S,Atak A,Bozdayi G,Turkcuoglu S,Maral I

    更新日期：2010-12-01 00:00:00

  • Isolation of Trichophyton concentricum from chronic cutaneous lesions in patients from the Solomon Islands.

    abstract：:Tinea imbricata, also known as 'Tokelau', is an uncommon superficial mycosis caused by the anthropophilic dermatophyte Trichophyton concentricum. Cutaneous lesions appear characteristically as scaly and concentric rings that may cover all parts of the body. Often acquired in childhood, tinea imbricata is a chronic dis...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2008.01.002

    authors： Pihet M,Bourgeois H,Mazière JY,Berlioz-Arthaud A,Bouchara JP,Chabasse D

    更新日期：2008-04-01 00:00:00

  • Characterization of Leishmania species from Peru.

    abstract：:Twenty-six isolates of Leishmania parasites of Peruvian origin were studied by isoenzyme electrophoresis of four marker enzymes (ASAT, ALAT, G6PD and GPI), kinetoplast DNA hybridization and monoclonal antibody binding and compared with marker strains of the New World organisms L. b. braziliensis, L. b. guyanensis, L. ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(87)90270-7

    authors： Romero GG,Arana M,López M,Montoya I,Bohl R,Campos M,Arévalo J,Llanos A

    更新日期：1987-01-01 00:00:00

  • Chlamydia trachomatis associated with chronic dysuria among patients with Schistosoma haematobium.

    abstract：:Eighty-seven Egyptians with Schistosoma haematobium infection (current or past) and chronic dysuria seen at the Theodor Bilharz Research Institute outpatient urology clinic in Imbaba, Cairo, Egypt were investigated for Chlamydia trachomatis infection. Direct fluorescent antibody tests (DFAT) showed 30 urethral swabs (...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(93)90284-w

    authors： Haberberger RL Jr,Mokhtar S,Badawy H,Abu-Elyazeed R

    更新日期：1993-11-01 00:00:00

  • The effect of adenosine analogues on the in vitro growth of Trypanosoma cruzi.

    abstract：:Trypanosoma cruzi (Brazil strain) was maintained in liver infusion tryptose and medium supplemented with 5 or 10% foetal calf serum at 27 degrees C on a rotating shaker platform. 85 to 95% of the organisms under these conditions are epimastigotes and the medium supported logarithmic growth for up to 24 hours. The effe...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(82)90169-9

    authors： Nadler JP,Lederer E,Wittner M,Baum SG,Tanowitz HB

    更新日期：1982-01-01 00:00:00

  • Tropical sprue in southern India.

    abstract：:Tropical sprue, a primary malabsorption syndrome affecting residents and visitors to several tropical regions, occurs in southern India in endemic and epidemic forms. The stomach, the small intestine and colon are affected and malabsorption results in nutrient deficiency. Enterocyte damage, the primary lesion in south...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1016/0035-9203(88)90247-7

    authors： Mathan VI

    更新日期：1988-01-01 00:00:00

  • Determination of anti-Cryptosporidium coproantibodies by time-resolved immunofluorometric assay.

    abstract：:The role of the mucosal immune response against Cryptosporidium has been suggested by studies on the therapeutic effects of hyperimmune colostrum. In order to study the intestinal response to this infection, we have developed a sandwich-type time-resolved immunofluorometric assay for the determination of anti-Cryptosp...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(93)90152-g

    authors： Kapel N,Meillet D,Buraud M,Favennec L,Magne D,Gobert JG

    更新日期：1993-05-01 00:00:00

  • Plasmodium falciparum: administration of anti-sporozoite antibodies during sporogony results in production of sporozoites which are not neutralized by human anti-circumsporozoite protein vaccine sera.

    abstract：:Five days after receiving a Plasmodium falciparum NF54 infectious blood meal, Anopheles stephensi mosquitoes were fed rat anti-P, falciparum sporozoite or rabbit anti-R32tet32 antibodies. Sporozoites isolated from salivary glands were tested by the inhibition of sporozoite invasion (ISI) assay using monoclonal antibod...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(89)90481-1

    authors： do Rosario VE,Appiah A,Vaughan JA,Hollingdale MR

    更新日期：1989-05-01 00:00:00

  • Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction.

    abstract：:Polymerase chain reaction (PCR) is now widely used in malaria research for analysis of field samples. However, little has been reported regarding loss of sensitivity due to field methodology. Therefore, studies were carried out in relation to blood sampling (anticoagulants, culture medium, filter paper), storage (temp...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,多中心研究

    doi：10.1016/s0035-9203(99)90177-3

    authors： Färnert A,Arez AP,Correia AT,Björkman A,Snounou G,do Rosário V

    更新日期：1999-01-01 00:00:00

  • Amino-acid and glucose utilization of Leishmania donovani and L. braziliensis.

    abstract：:The availability of a defined medium (STEIGER & STEIGER, 1976) has enabled studies on the nutritional requirements of promastigotes of L. donovani and L. braziliensis (STEIGER & STEIGER, 1977). As outlined elsewhere (STEIGER & MESHNICK, 1977), the present paper describes preliminary experiments dealing with the utiliz...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(77)90049-9

    authors： Steiger RF,Meshnick SR

    更新日期：1977-01-01 00:00:00

  • Natural swarming behaviour of the molecular M form of Anopheles gambiae.

    abstract：:In Anopheles gambiae, as in most species of mosquitoes, mating is initiated in flight. The males aggregate in aerial swarms and conspecific females individually fly to these swarms where they mate with males. In this study, we investigated the swarming behaviour of A. gambiae and conducted 2 surveys in the rice field ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/s0035-9203(03)80110-4

    authors： Diabate A,Baldet T,Brengues C,Kengne P,Dabire KR,Simard F,Chandre F,Hougard JM,Hemingway J,Ouedraogo JB,Fontenille D

    更新日期：2003-11-01 00:00:00

  • Markers of poor adherence among adults with HIV attending Themba Lethu HIV Clinic, Helen Joseph Hospital, Johannesburg, South Africa.

    abstract：Background:To date, there is no consensus on ideal ways to measure antiretroviral treatment (ART) adherence in resource limited settings. This study aimed to identify markers of poor adherence to ART. Methods:Retrospective data of HIV-positive ART-naïve adults initiating standard first-line ART at Themba Lethu Clinic,...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/trx003

    authors： Nnambalirwa M,Govathson C,Evans D,McNamara L,Maskew M,Nyasulu P

    更新日期：2016-12-01 00:00:00

  • Audiometry as a possible indicator of quinine plasma concentration during treatment of malaria.

    abstract：:The spread of chloroquine-resistant malaria has led to a resurgence of quinine in clinical use. One of the well-known side effects of quinine, reversible hearing loss, is closely related to the plasma concentration. We suggest that this hearing effect could be used as an aid in therapy control when quinine drug assay ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(90)90069-q

    authors： Karlsson KK,Hellgren U,Alván G,Rombo L

    更新日期：1990-11-01 00:00:00

  • Molecular characterisation of the first New Delhi metallo-β-lactamase 1-producing Acinetobacter baumannii from Tanzania.

    abstract：BACKGROUND:We aimed to characterise the genetic determinants and context of two meropenem-resistant clinical isolates of Acinetobacter baumannii isolated from children hospitalised with bloodstream infections in Dar es Salaam, Tanzania. METHODS:Antimicrobial susceptibility was determined by disc diffusion E-test and b...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/traa173

    authors： Moyo SJ,Manyahi J,Hubbard ATM,Byrne RL,Masoud NS,Aboud S,Manji K,Blomberg B,Langeland N,Roberts AP

    更新日期：2021-01-27 00:00:00

  • Malaria in the Nuba Mountains of Sudan: baseline genotypic resistance and efficacy of the artesunate plus sulfadoxine-pyrimethamine and artesunate plus amodiaquine combinations.

    abstract：:Both northern and southern Sudan are deploying artemisinin-based combinations against uncomplicated Plasmodium falciparum malaria (artesunate+sulfadoxine-pyrimethamine [AS+SP] in the north, artesunate+amodiaquine [AS+AQ] in the south). In 2003, we tested the efficacy of 3 day AS+SP and AS+AQ regimens in vivo in the is...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.trstmh.2004.10.003

    authors： Hamour S,Melaku Y,Keus K,Wambugu J,Atkin S,Montgomery J,Ford N,Hook C,Checchi F

    更新日期：2005-07-01 00:00:00