Abstract:
BACKGROUND:Altered expression of murine MD-1, a molecule controlling expression of members of the interleukin (IL)-1 receptor family of signaling proteins, regulates antigen-presenting cell-induced alloreactions. We investigated the effect of treatment with antisense deoxyoligonucleotides or antibodies to MD-1 in vivo on allogeneic and xenogeneic skin graft survival and the immune responses in transplanted mice. METHODS:C3H mice received C57BL/6 or Lewis rat skin grafts, followed by i.v. injections of anti-MD-1 antibody or antisense oligonucleotides or control reagents at 48-hr intervals. Survival was monitored. In separate studies, mice were sacrificed at 5-day intervals. Serum was analyzed for circulating MD-1 antigen, and peritoneal cells for surface expression of MD-1. The proliferative and cytolytic response of lymphocytes harvested from treated animals and restimulated in vitro with allo- or xenogeneic cells, and the cytokines produced, was measured. Graft histology was assessed at 11 days after transplantation. RESULTS:Treatment with anti-MD-1 oligonucleotides or antibodies suppressed rejection of both xeno- and allogeneic grafts, decreased induction of graft-specific cytotoxic T cells, increased production of type-2 cytokines (IL-4 and IL-10), and decreased production of type-1 cytokines (IL-2 and interferon-gamma). Serum levels of MD-1 were suppressed, as was expression of MD-1 on the surface of antigen-presenting cells. Grafts from MD-1-treated mice showed little lymphocyte infiltration, and no signs of graft necrosis. CONCLUSION:Our data suggest a critical in vivo role for MD-1 expression in regulating graft rejection, as well as in the concomitant sensitization of T cells and their cytokine production profile, which parallels the rejection response.
journal_name
Transplantationjournal_title
Transplantationauthors
Hadidi S,Chen Z,Phillips J,Yu K,Gorczynski RMdoi
10.1097/00007890-200206150-00013subject
Has Abstractpub_date
2002-06-15 00:00:00pages
1771-9issue
11eissn
0041-1337issn
1534-6080journal_volume
73pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Treatment with a donor-specific transfusion (DST) and a brief course of anti-mouse CD154 (anti-CD40-ligand) monoclonal antibody (mAb) prolongs the survival of both allografts and rat xenografts in mice. The mechanism by which allograft survival is prolonged is incompletely understood, but depends in part on ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200101270-00026
更新日期:2001-01-27 00:00:00
abstract::The role of TNF in the expression of GVHD and GVHD-related immunodeficiency was studied in a well-established murine GVHD model of bone marrow transplantation across minor histocompatibility barriers (B10.BR-->GBA/J) both in vitro and in vivo. Splenocytes from animals with GVHD profoundly inhibited the proliferation o...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199401001-00021
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Auxiliary partial orthotopic liver transplantation (APOLT) has been an effective alternative in acute liver failure (ALF), but clinically several problems remain to be resolved. Thus, we attempt to establish an APOLT model for ALF using a large animal and demonstrate the validity of our model. METHODS:In ex...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000243360.64554.fa
更新日期:2006-11-27 00:00:00
abstract:BACKGROUND:Immunosuppression involves the nature of the immunosuppressive agents and individual differences in patient factors. We investigated whether the effect of mycophenolate mofetil (MMF) is measurable using an in vitro measure of immunocompetence and related its effects to cyclosporine (CsA) in vitro. METHODS:L...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200108150-00011
更新日期:2001-08-15 00:00:00
abstract::Patient entry is now complete in a prospective trial of anti-Tac, a murine IgG2a monoclonal antibody directed against the p55 chain of the human IL-2 receptor, for the prevention of renal allograft rejection. Recipients of primary cadaver allografts were randomized to receive either anti-Tac (20 mg q.d. x 10 days begi...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199101000-00016
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:The strategy of evaluating every donation opportunity warrants an investigation into the financial feasibility of this practice. The purpose of this investigation is to measure resource utilization required for procurement of transplantable organs in an organ procurement organization (OPO). METHODS:Donors w...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000441093.32217.cb
更新日期:2014-05-27 00:00:00
abstract:BACKGROUND:After cold ischemia, electrons transferred in the electron transport chain may leak out of the mitochondria in proportion to the deterioration of mitochondrial oxidative phosphorylation. This seems to be one major cause of the lipid peroxidation that occurs mainly in the hepatocytes at reperfusion in liver t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199904270-00015
更新日期:1999-04-27 00:00:00
abstract::Three hundred thirty-two marrow graft recipients and 241 healthy marrow donors were studied by skin testing with recall and neoantigens. Two hundred thirty patients with leukemia and seventy-eight patients with aplastic anemia received allogeneic HLA-identical sibling marrow. Twenty-four patients with leukemia receive...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198402000-00006
更新日期:1984-02-01 00:00:00
abstract::In human organ transplantation, the correct diagnosis of rejection may be difficult using clinical and/or histopathologic criteria, and immunologic assays should therefore be considered. We have applied monoclonal antibodies in 2-color flow cytometric (FACS) analysis to study phenotypic patterns of kidney-infiltrating...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198905000-00014
更新日期:1989-05-01 00:00:00
abstract:BACKGROUND:Kidney donation after cardiac death leads to vascular damage as a result of warm ischemia, affecting renovascular circulating volume. Novel ultrasound dilution techniques may be used to measure renovascular circulating volumes during hypothermic machine perfusion of donor kidneys. METHODS:Renovascular circu...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31828a46f7
更新日期:2013-05-15 00:00:00
abstract::There is considerable current interest in the possibility that long-term graft acceptance in clinical solid-organ transplantation might be dependent upon the development of a microchimeric state between the donor and recipient. This possibility has been prompted by the observation that in some transplant patients cell...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199505270-00001
更新日期:1995-05-27 00:00:00
abstract:BACKGROUND:Thrombotic microangiopathy (TMA) is a fatal complication characterized by microvascular occlusive disorder resulting in systemic or intrarenal platelet aggregation, severe thrombocytopenia, and microangiopathic hemolytic anemia. Only sporadic case reports of TMA after cadaveric or living-donor liver transpla...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000165048.00487.8e
更新日期:2005-07-27 00:00:00
abstract::The effect of cyclosporine and metabolite 17 (M17) as well as other CsA-related compounds (CsG, dihydro-CsC, dihydro-CsD, CsH, B5.49, and H7.94) was tested on T lymphocyte clone proliferation. In these experiments, antigen and interleukin 2 (IL-2) dependent long-term T lymphocyte clones derived from a rejected human k...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198712000-00019
更新日期:1987-12-01 00:00:00
abstract::Frozen sections of lymph nodes from normal mice were examined by immunofluorescence with anti-T lymphocyte and anti-Ig reagents and by autoradiography following [3H]TdR administration. The scattered T lymphocytes present among B lymphocytes of primary follicles were found to be mostly nonproliferating; the few prolife...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198111000-00018
更新日期:1981-11-01 00:00:00
abstract:BACKGROUND:Better preservation strategies for the storage of donation after circulatory death grafts are essential to improve graft function and to increase the kidney donor pool. We compared continuous normothermic ex vivo kidney perfusion (NEVKP) with hypothermic anoxic machine perfusion (HAMP) and static cold storag...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003066
更新日期:2020-05-01 00:00:00
abstract::To establish the impact of transplantation on the course of chronic hepatitis B liver disease we performed a prospective study of the clinical and pathological sequelae of hepatitis B disease in all 22 patients who had renal allografts that functioned for more than 1 year and who were hepatitis B surface antigen (HBsA...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198506000-00007
更新日期:1985-06-01 00:00:00
abstract::A murine IgG1 antibody specific for the IL-2-binding site on the human lymphocyte IL-2 receptor beta chain (CD25) was evaluated in 11 patients who developed acute graft-versus-host disease following allogeneic marrow transplantation. All patients had received cyclosporine and methotrexate for prophylaxis of GVHD, eith...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199007000-00010
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:Kidneys from older donors are likely to have a lower nephron mass. Nevertheless they constitute a valuable source of kidney allografts. Long cold ischemic time (CIT), with or without delayed graft function (DGF), has been associated with reduced graft survival. The aim of this study was to review the experie...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200108270-00020
更新日期:2001-08-27 00:00:00
abstract::A total of 159 patients received renal cadaveric grafts under 2 different allocation systems. System 1, a local variance (i.e., a point system different from that of United Network for Organ Sharing [UNOS]), and system 2, the current UNOS point system, differed in the relative emphasis on waiting time and HLA match. T...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-04-15 00:00:00
abstract::The methodology of the conventional mixed lymphocyte culture test has been simplified and miniaturized by developing a micromethod employing Terasaki trays with a final culture volume of 20 mul containing between 10,000 and 20,000 responding and stimulating cells each/culture. This represents one-tenth the cell concen...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197507000-00005
更新日期:1975-07-01 00:00:00
abstract:BACKGROUND:Increased use of pediatric deceased donor kidneys could enlarge the deceased donor kidney pool. Kidney transplant outcomes from small pediatric donors were compared with those from ideal kidney (IK) and expanded criteria kidney (ECK) donors to understand the optimal use of pediatric donor kidneys. METHODS:K...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0000000000002706
更新日期:2019-12-01 00:00:00
abstract::Little is known of the host immune mechanisms responsible for initiation and progression of chronic rejection. We describe immunopathologic features associated with progressively deteriorating function of kidney allografts in the F344-to-Lewis rat strain combination, which differ at MHC and non-MHC loci. Initial rejec...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199309000-00028
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:Prophylactic administration of anti-HBs intravenous immunoglobulins (IVIg) in hepatitis B infected-liver transplant patients protects against acute rejection. To explore the suitability of intravenous immunoglobulins (IVIg) as prophylaxis of acute rejection and graft-versus-host disease (GVHD) after allograf...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000226073.20185.b1
更新日期:2006-06-27 00:00:00
abstract:BACKGROUND:The established method of pretransplant cross-matching does not detect IgA antibodies, and IgA antibodies have thus been ignored when assessing patients for transplantation. The aim of this study was to detect IgA allo- and autoreactive antibodies using flow cytometry and to correlate the results with transp...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199901270-00022
更新日期:1999-01-27 00:00:00
abstract::Incompatibility for DBA/2 (D2) minor histocompatibility antigens (MiHA) alone leads to a severe lethal graft-versus-host reaction (GVHR) in irradiated (D2 X B10.D2)F1 mice receiving a bone-marrow-plus-spleen-cell graft from B10.D2 donors. This mortality is abrogated when the donors are preimmunized simultaneously agai...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198309000-00016
更新日期:1983-09-01 00:00:00
abstract::Previous studies in our laboratory demonstrated that several treatments that inhibited the ability of cells to stimulate the mixed lymphocyte reaction (MLR) also blocked the shedding of histocompatibility antigens and Ia antigens from murine spleen cells. In the present studies, one of these treatments, ultraviolet ra...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198502000-00013
更新日期:1985-02-01 00:00:00
abstract:BACKGROUND:T-cell activation through T-cell receptor engagement requires co-stimulatory molecules and also adhesion molecules such as ICAM-1. Moreover ICAM-1 mediates leukocyte invasion from the blood into tissue during inflammatory processes. In animal studies using mouse monoclonal antibodies against ICAM-1 (enlimoma...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/00007890-199903150-00015
更新日期:1999-03-15 00:00:00
abstract::A novel method for quantifying the fatty change of the graft liver by characterizing the optical property of the tissue was introduced. A wide range of lipid content in the rat liver was obtained by using different feeding regimens, with lipotropic chow (choline/methionine deficient or low chow). The liver was removed...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199609150-00018
更新日期:1996-09-15 00:00:00
abstract::The H-Y (male) antigen is detectable on 50% of eight-cell mouse embryos. We show that 82% of the offspring resulting from the transfer of (C57BL/6 X A/J)F1 embryos surviving treatment with H-Y antibody and complement to the uteruses of pseudopregnant mice are female. The increased ratio of female-to-male births indica...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198401000-00004
更新日期:1984-01-01 00:00:00
abstract::The dose-response effect of cyclosporine on rat limb transplant prolongation was investigated across the LBN-to-LEW histocompatibility barrier. This composite tissue allograft model has been shown to represent a strong transplantation barrier. Median limb allograft survival times increased in a dose-dependent manner w...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198504000-00004
更新日期:1985-04-01 00:00:00