Abstract:
:The methodology of the conventional mixed lymphocyte culture test has been simplified and miniaturized by developing a micromethod employing Terasaki trays with a final culture volume of 20 mul containing between 10,000 and 20,000 responding and stimulating cells each/culture. This represents one-tenth the cell concentration of the conventional mixed lymphocyte culture. For this new test, a special microharvester has been designed and developed. The test allows accurate and reproducible results giving a high level of discrimination between the different levels of response.
journal_name
Transplantationjournal_title
Transplantationauthors
Péna-Martinez J,Festenstein Hdoi
10.1097/00007890-197507000-00005subject
Has Abstractpub_date
1975-07-01 00:00:00pages
26-37issue
1eissn
0041-1337issn
1534-6080journal_volume
20pub_type
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