Abstract：
:Chromatid breaks have previously been shown to be induced in G2-phase cells after exposure to ionizing radiation (X and gamma rays) as a linear function of dose, consistent with a single-event mechanism. DNA double-strand breaks (DSBs) are thought to be the initiating lesion, and experiments with a genetically engineered cell line containing a single DSB site also indicate that a single DSB is sufficient to induce a chromatid break. Although the precise mechanism of conversion of an isolated DSB into a chromatid break is not yet understood, it is known that a proportion of chromatid breaks result from rearrangements between sister chromatids. Here we report further evidence for the single-event hypothesis for the formation of chromatid breaks. The evidence derives from experiments in which chromatid breaks have been induced by exposure of Chinese hamster cells to ultrasoft carbon K-shell X rays. Since the energy of carbon K-shell X rays is not sufficient for the secondary electrons to span more than one DNA double helix, we conclude that single traversals, and hence single (complex) DSBs, are responsible for the formation of chromatid breaks. We find that, as for 60Co gamma rays, around 10% of the carbon K-shell X-ray-induced chromatid breaks have associated color switches at breakpoints, indicating that they arise through sister chromatid rearrangements.
journal_name
Radiat Resjournal_title
Radiation researchauthors
Bryant PE,Jones C,Armstrong G,Frankenberg-Schwager M,Frankenberg Ddoi
10.1667/0033-7587(2003)159[0247:iocbbc]2.0.co;2subject
Has Abstractpub_date
2003-02-01 00:00:00pages
247-50issue
2eissn
0033-7587issn
1938-5404journal_volume
159pub_type
杂志文章abstract：:The susceptibility to inducible SOS repair of 5,6-dihydroxy-dihydrothymine (t') damage in single-stranded phi X174 DNA has been measured. Following exposure to osmium tetroxide, which introduces t' damage in DNA under the conditions used, biological survival of the DNA infected into spheroplasts of Escherichia coli wh...
journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
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journal_title：Radiation research
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doi：
更新日期：1990-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1968-11-01 00:00:00
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doi：10.1667/RR13584.1
更新日期：2014-06-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
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pub_type： 杂志文章,评审
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doi：10.1667/rr3360
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR14884.1
更新日期：2018-04-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
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更新日期：2016-08-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/rr3085
更新日期：2003-12-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/0033-7587(2002)157[0386:acsisf]2.0.co;2
更新日期：2002-04-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR1317.1
更新日期：2008-07-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR3529.1
更新日期：2006-06-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：10.1667/RR2888.1
更新日期：2012-11-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
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更新日期：2019-05-01 00:00:00
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journal_title：Radiation research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1667/0033-7587(2003)159[0550:eotsit]2.0.co;2
更新日期：2003-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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更新日期：2008-04-01 00:00:00
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