Abstract：
:Recent developments in stem cell and gene therapy will require methods to monitor stem cell survival and integration repeatedly and non-invasively with a high temporal and spatial resolution in vivo. The aim of this study was to visualise embryonic and neuronal stem cells with standard contrast agents using a conventional clinical 1.5-Tesla scanner. We therefore modified standard transfection protocols including lipofection (Lipofectin and Lipofectamine) and calcium phosphate transfection for the efficient uptake of paramagnetic particles [gadolinium-diethylene triamine penta-acetic acid (Gd-DTPA)] in stem cells. Using this approach we obtained intracellular labelling efficiencies of up to 83%. Neither the proliferation capacity nor the differentiation efficiency was affected. Identical differentiation of labelled and unlabelled embryonic and neuronal cells was observed. The established labelling techniques used in this study displayed high labelling efficiencies in embryonic and neuronal stem cells without any alterations of cellular biology; therefore this approach might be a suitable method for targeting stem cells.
journal_name
Eur J Nucl Med Mol Imagingauthors
Rudelius M,Daldrup-Link HE,Heinzmann U,Piontek G,Settles M,Link TM,Schlegel Jdoi
10.1007/s00259-002-1110-0subject
Has Abstractpub_date
2003-07-01 00:00:00pages
1038-44issue
7eissn
1619-7070issn
1619-7089journal_volume
30pub_type
杂志文章abstract：PURPOSE:The aim of this short communication is to outline our experience in policies and processes of a nuclear medicine service during the COVID-19 outbreak in Singapore. METHODS:We describe the key considerations of policies and processes that have been implemented in our nuclear medicine service since the first cas...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-020-04790-2
更新日期：2020-07-01 00:00:00
abstract：PURPOSE:Hypometabolism of the posterior cingulate cortex (PCC) in early Alzheimer's disease (AD) is thought to arise in part due to AD-specific neuronal damage to the hippocampal formation. Here, we explored the association between microstructural alterations within the hippocampus and whole-brain glucose metabolism in...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-011-1882-1
更新日期：2011-12-01 00:00:00
abstract：PURPOSE:The aim of this study was to investigate metabolic and textural parameters from pretreatment [(18)F]FDG PET/CT scans for the prediction of neoadjuvant radiation chemotherapy response and 3-year disease-free survival (DFS) in patients with locally advanced rectal cancer (LARC). METHODS:We performed a retrospect...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-015-3180-9
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:This meta-analysis summarized the accuracy of stress myocardial perfusion scintigraphy (MPS) and stress echocardiography for the diagnosis of coronary artery disease (CAD) in patients with arterial hypertension. METHODS:We searched for studies in which stress MPS or stress echocardiography were performed to de...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,meta分析
doi：10.1007/s00259-011-1891-0
更新日期：2011-11-01 00:00:00
abstract：:Assessing joint disorders has been a relatively recent and evolving application of 18F-2-fluoro-2-deoxy-D-glucose (FDG) positron emission tomography (PET) imaging. FDG is taken up by inflammatory cells, particularly when they are active as part of an ongoing inflammatory process. Hence FDG PET has been employed to ass...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-011-1863-4
更新日期：2011-10-01 00:00:00
abstract：PURPOSE:Chemoradiotherapy is the reference curative-intent treatment for nonresectable locally advanced non-small-cell lung carcinoma (NSCLC), with unsatisfactory survival, partially due to radiation resistance in hypoxic tissues. The objective was to update survival and toxicity at 3 years following radiotherapy boost...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,多中心研究
doi：10.1007/s00259-019-04285-9
更新日期：2019-07-01 00:00:00
abstract：BACKGROUND:The clinical problem in suspected aortoiliac graft infection (AGI) is to obtain proof of infection. Although 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography scanning (PET) has been suggested to play a pivotal role, an evidence-based interpretation is lacking. The objective of this retrospective...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-016-3599-7
更新日期：2017-05-01 00:00:00
abstract：PURPOSE:The aim of this study was to determine whether the follow-up of pain processing recovery in hyperalgesic fibromyalgia (FM) could be objectively evaluated with brain perfusion ethyl cysteinate dimer single photon computerized tomography (ECD-SPECT) after administration of ketamine. MATERIALS AND METHODS:We enro...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0589-9
更新日期：2007-12-01 00:00:00
abstract：PURPOSE:We investigated the performance of the Inveon small-animal PET/SPECT/CT system and compared the imaging capabilities of the SPECT and PET components. METHODS:For SPECT, the energy resolution, tomographic spatial resolution and system sensitivity were evaluated with a (99m)Tc solution using a single pinhole col...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-010-1683-y
更新日期：2011-04-01 00:00:00
abstract：PURPOSE:Physiological glucose uptake by the myocardium may hamper visualization of coronary atherosclerotic plaques in (18)F-FDG PET studies. Intracellular myocardial calcium relates to glucose influx. We assessed whether administration of a calcium channel blocker such as verapamil could decrease myocardial (18)F-FDG ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-011-1873-2
更新日期：2011-11-01 00:00:00
abstract：PURPOSE:Despite modern CT systems and expert evaluators, the diagnostic performance of coronary CT angiography is limited by overestimation of vessel stenosis which reduces the positive predictive value (PPV) of the test. The aim of this study was to evaluate the performance of combined cardiac PET/64-detector CT angio...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-009-1213-y
更新日期：2009-12-01 00:00:00
abstract：INTRODUCTION:Fluorescence guidance is an upcoming methodology to improve surgical accuracy. Challenging herein is the identification of the minimum dose at which the tracer can be detected with a clinical-grade fluorescence camera. Using a hybrid tracer such as indocyanine green (ICG)-(99m)Tc-nanocolloid, it has become...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-016-3372-y
更新日期：2016-09-01 00:00:00
abstract：:We assessed the accuracy of brain perfusion single-photon emission computed tomography (SPECT) in discriminating between patients with probable Alzheimer's disease (AD) at the very early stage and age-matched controls before and after partial volume correction (PVC). Three-dimensional MRI was used for PVC. We randomly...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00259-004-1491-3
更新日期：2004-07-01 00:00:00
abstract：PURPOSE:Genes encoding for intracellular enzymes or transmembrane proteins are suitable as reporters, but may differ in terms of applicability for cardiac imaging. The aim of this study was to compare the human sodium iodide symporter gene (hNIS) with the herpes simplex virus type 1 thymidine kinase gene (HSV1-tk) as t...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-005-1854-4
更新日期：2005-09-01 00:00:00
abstract：:Paragangliomas or glomus tumours of the head and neck region are rare somatostatin receptor-expressing neuroendocrine tumours. Precise preoperative diagnosis is of special importance in order to adequately weigh the potential benefit of the operation against the inherent risks of the procedure. In this study, the clin...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-002-0939-6
更新日期：2002-12-01 00:00:00
abstract：PURPOSE:Dual-isotope simultaneous acquisition single-photon emission computed tomography (DISA SPECT) with 18F-fluorodeoxyglucose (FDG) and (99m)Tc-sestamibi appears attractive for the detection of viable myocardium because it permits simultaneous assessment of glucose utilisation and perfusion. Another potential benef...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-004-1668-9
更新日期：2005-02-01 00:00:00
abstract：PURPOSE:This study investigated the diagnostic performance and prognostic value of fluorodeoxyglucose (FDG) positron emission tomography (PET)/CT in suspected malignant pleural mesothelioma (MPM) recurrence, in the context of patterns and intensity of FDG uptake, histologic type, and treatment algorithm. METHODS:Fifty...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-010-1704-x
更新日期：2011-05-01 00:00:00
abstract：PURPOSE:We compared left ventricular (LV) ejection fraction obtained by gated SPECT with that obtained by equilibrium radionuclide angiocardiography in a large cohort of patients. METHODS:Within 1 week, 514 subjects with suspected or known coronary artery disease underwent same-day stress-rest (99m)Tc-sestamibi gated ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-009-1308-5
更新日期：2010-02-01 00:00:00
abstract：PURPOSE:[(18)F]Fluoro-3'-deoxy-3'-L-fluorothymidine ([(18)F]FLT) is a tissue proliferation marker which has been widely validated as a tumour-specific imaging tracer for PET. [(18)F]FLT uptake in breast cancer is generally quantified at the region level or through first-order statistical descriptors (mean or maximum va...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-014-2757-z
更新日期：2014-09-01 00:00:00
abstract：:The iodinated cocaine derivative [(123)I]PE2I is a new selective ligand for in vivo studies of the dopamine transporter (DAT) with single-photon emission tomography (SPET). The aim of the present study was to describe a method for accurate quantification of binding data following a bolus injection of [(123)I]PE2I. Six...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-001-0742-9
更新日期：2002-05-01 00:00:00
abstract：PURPOSE:To investigate the value of 68Ga-HBED-CC PSMA (68Ga-PSMA) PET/CT for response assessment in metastatic castration-sensitive and castration-resistant prostate cancer (mCSPC and mCRPC) during docetaxel chemotherapy. METHODS:68Ga-PSMA PET/CT was performed in seven mCSPC patients before and after six cycles of upf...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3887-x
更新日期：2018-04-01 00:00:00
abstract：PURPOSE:In single-photon emission computed tomography (SPECT) of the dopaminergic system, measurements of striatal uptake are useful for diagnosis and patient follow-up but are strongly biased by the partial volume effect (PVE). We studied whether PVE correction might improve patient classification based on binding pot...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-005-0003-4
更新日期：2006-09-01 00:00:00
abstract：:Functional imaging of small animals, such as mice and rats, using ultra-high resolution positron emission tomography (PET) and single-photon emission tomography (SPET), is becoming a valuable tool for studying animal models of human disease. While several studies have shown the utility of PET imaging in small animals,...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-002-0776-7
更新日期：2002-05-01 00:00:00
abstract：PURPOSE:To investigate whether (11)C-N-methylspiperone ((11)C-NMSP) microPET could be used for imaging neural stem cells (NSCs) transplantation in a rat model of traumatic brain injury. METHODS:NSCs were induced to express dopamine receptor type 2 (DRD(2)), then confirmed by RT-PCR, Western blotting and immunocytochem...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-008-0835-9
更新日期：2008-09-01 00:00:00
abstract：:Techniques from the field of artificial intelligence, and more specifically machine (deep) learning methods, have been core components of most recent developments in the field of medical imaging. They are already being exploited or are being considered to tackle most tasks, including image reconstruction, processing (...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-019-04373-w
更新日期：2019-12-01 00:00:00
abstract：UNLABELLED:The aim of this study was to evaluate the feasibility of using [(18)F] 3'-deoxy-3'-fluorothymidine (FLT) positron emission tomography (PET) for the diagnosis and grading of brain tumors. METHODS:The patient population comprised 26 patients (15 males, 11 females) with brain tumors (n=18) or nontumorous lesio...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-004-1742-3
更新日期：2005-06-01 00:00:00
abstract：PURPOSE:The purpose of this study was to compare dynamic 18F-FGln PET/CT images of healthy subjects and cancer patients and explore the best imaging phase for different cancers. METHODS:Thirteen healthy volunteers and 31 cancer patients separately underwent 18F-FGln and 18F-FDG PET/CT scans within 1 week. The distribu...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-019-04543-w
更新日期：2020-09-01 00:00:00
abstract：:As measurement of glomerular filtration rate (GFR) is now generally the responsibility of departments of nuclear medicine, it is important for nuclear medicine physicians and scientists to understand the pharmacokinetics of the indicators and radiotracers that are used, generally known as filtration markers. The singl...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-003-1341-8
更新日期：2004-01-01 00:00:00
abstract：PURPOSE:Positron emission tomography (PET)/MRI combines the functional ability of PET and the high soft tissue contrast of MRI. The aim of this study was to assess contrast-enhanced (ce)PET/MRI compared to cePET/CT in patients with suspected recurrence of head and neck cancer (HNC). METHODS:Eighty-seven patients under...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-014-2707-9
更新日期：2014-06-01 00:00:00
abstract：:The goal of this study was to perform a systematic, semi-quantitative analysis of limbic perfusion in patients with Alzheimer's disease (AD) using coregistered single-photon emission tomography (SPET) images aligned to magnetic resonance (MR) images. Limbic perfusion in 40 patients with mild to moderate AD was compare...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-002-0816-3
更新日期：2002-07-01 00:00:00
