Molecular basis for multiple resistance to acetolactate synthase-inhibiting herbicides and atrazine in Amaranthus blitoides (prostrate pigweed).

Abstract:

:Amaranthus blitoides S. Watson (prostrate pigweed) populations resistant to acetolactate synthase (ALS; EC 4.1.3.18)-inhibiting herbicides and triazines (SuR/TR) were found in Israel. The Ganot population was 6- to 790-fold more resistant to ALS inhibitors than the wild type due to an altered target site. Molecular analyses showed that the Ganot population was a mixture of two biotypes: (i) SuRA/TR in which domain A of the als gene differed in one nucleotide, resulting in substitution of Pro by Ser 188; (ii) SuRB/TR in which a mutation in domain B led to a substitution of Trp by Leu 569. The mutation in domain A resulted in resistance to all ALS inhibitors except imidazolinones, whereas the mutation in domain B led to resistance to all ALS inhibitors tested. SuRA/TR and SuRB/TR are multiple-resistant with an additional single mutation in the plastidic psbA gene that changes Ser 264 to Gly in the D1 protein, leading to triazine resistance. It is evident that plants within a population exposed to a similar selection pressure may show different patterns of cross-resistance due to three different point mutations. This unique phenomenon renders planning of rational weed management difficult or even impossible.

journal_name

Planta

journal_title

Planta

authors

Sibony M,Rubin B

doi

10.1007/s00425-002-0955-6

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

1022-7

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

216

pub_type

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