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A new direct method for determining superoxide dismutase activity by measuring hydrogen peroxide formation.

Abstract：

:A new, direct method for determining superoxide dismutase activity is presented in this study. This method is based on measurement of one of the products of the superoxide dismutase reaction, hydrogen peroxide. Hydrogen peroxide is quantitated using a coupled reaction where horseradish peroxidase catalyzes the formation of a fluorescent product, 6,6'-diOH-(1,1'-biphenyl)-3,3'-diacetic acid, from 4-OH-phenylacetic acid and hydrogen peroxide. Substrate for superoxide dismutase is provided by reduction of oxygen during the autoxidation of riboflavin in the presence of UV light. A linear correlation between the amount of superoxide dismutase (200 ng-6 micrograms) and of hydrogen peroxide was found with this method.

journal_name

Chem Biol Interact

journal_title

Chemico-biological interactions

authors

Segura-Aguilar J

doi

10.1016/0009-2797(93)90112-c

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

69-78

issue

1

eissn

0009-2797

issn

1872-7786

pii

0009-2797(93)90112-C

journal_volume

86

pub_type

杂志文章

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