Immunohistochemical expression of laminin-5 in cervical intraepithelial neoplasia.


OBJECTIVES:Laminin-5 is an attachment protein for epithelial cells. Several studies of a variety of cancers have reported increased expression of laminin-5 in carcinoma in situ and invasive cancer. This study was designed to investigate the correlation between the grade of cervical intraepithelial neoplasia and the immunohistochemical expression of laminin-5 in the cytoplasm and in the basement membrane underlining dysplastic squamous cells. METHODS:We used immunohistochemical methods to stain paraffin-embedded sections of cervical cone biopsies with a monoclonal antibody specifically targeting the 2-chain of human laminin-5 protein. The study sample included 175 slides: 7 normal cervical epithelium, 36 lesions of mild dysplasia, 50 lesions of moderate dysplasia, 81 lesions of severe dysplasia, and 1 invasive squamous cell carcinoma. RESULTS:We found a statistically significant correlation between the grade of cervical intraepithelial neoplasia and laminin-5 immunoreactivity in the cytoplasm (P < 0.01) and in the basement membrane (P = 0.03) by use of the Wilcoxon rank-sum test. CONCLUSIONS:According to previously published reports we confirmed with a higher number of cases a correlation of laminin-5 expression in the cytoplasm and/or basement membrane and grade of dysplastic lesion in the cervical epithelium. This study warrants further investigations with special interest to follow-up to investigate whether laminin-5 is a marker to predict the risk of progression of cervical intraepithelial neoplasia lesions.


Gynecol Oncol


Gynecologic oncology


Kohlberger P,Beneder Ch,Horvat R,Leodolter S,Breitenecker G




Has Abstract


2003-06-01 00:00:00














  • 53BP1 expression in sporadic and inherited ovarian carcinoma: Relationship to genetic status and clinical outcomes.

    abstract:OBJECTIVES:53BP1, a critical mediator of the DNA damage response, functions by regulating the balance between homologous recombination (HR) and the more error-prone non-homologous endjoining (NHEJ). Deletion of 53BP1 in brca1 (but not brca2) null cells partially restores HR and reverses sensitivity to poly-ADP-ribose p...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Pennington KP,Wickramanayake A,Norquist BM,Pennil CC,Garcia RL,Agnew KJ,Taniguchi T,Welcsh P,Swisher EM

    更新日期:2013-03-01 00:00:00

  • Analysis of telomerase activity in ovarian cystadenomas, low-malignant-potential tumors, and invasive carcinomas.

    abstract:OBJECTIVE:Inappropriate telomerase expression has been reported to be associated with the development and/or progression of malignancies. Therefore, the purpose of the study was to determine and evaluate the levels of telomerase activity in normal ovary, cystadenomas, low-malignant-potential tumors, and carcinomas of t...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Datar RH,Naritoku WY,Li P,Tsao-Wei D,Groshen S,Taylor CR,Imam SA

    更新日期:1999-09-01 00:00:00

  • The validity and significance of substage IIIC by node involvement in epithelial ovarian cancer: impact of nodal metastasis on patient survival.

    abstract:OBJECTIVE:The aim of this study was to examine whether there is a possible difference in patient survival (1) between node-positive and node-negative groups for Stage I, II, IIIA+B, and IIIC disease and (2) between node-positive Stage I through IIIB disease and Stage IIIC disease. METHODS:Medical records of 125 patien...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Kanazawa K,Suzuki T,Tokashiki M

    更新日期:1999-05-01 00:00:00

  • Quality of life and treatment response among women with platinum-resistant versus platinum-sensitive ovarian cancer treated for progression: a prospective analysis.

    abstract:OBJECTIVE:Most women with ovarian cancer relapse and undergo further chemotherapy however evidence regarding the benefits of this for women with platinum-resistant disease is limited. Our objective was to determine whether there was a quality of life improvement or treatment response among women treated for platinum-re...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Beesley VL,Green AC,Wyld DK,O'Rourke P,Wockner LF,deFazio A,Butow PN,Price MA,Horwood KR,Clavarino AM,Australian Ovarian Cancer Study Group,Australian Ovarian Cancer Study-Quality Of Life Study Investigators,Webb PM

    更新日期:2014-01-01 00:00:00

  • Granulosa cell tumor of ovary: a clinicopathologic and flow cytometric DNA analysis.

    abstract::Flow cytometric DNA ploidy and S-phase fraction (SPF) analysis was performed on 18 granulosa cell tumors of the ovary. Clinical and pathologic data from patients followed for an average of 10 years were compared to flow cytometry ploidy to determine its prognostic usefulness. Eleven (62%) tumors were diploid and seven...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Roush GR,el-Naggar AK,Abdul-Karim FW

    更新日期:1995-03-01 00:00:00

  • The Wertheim hysterectomy: Development, modifications, and impact in the present day.

    abstract::Ernst Wertheim was a pioneer in the history of the surgical treatment of cervical cancer. His English-language manuscript "The extended abdominal operation for carcinoma uteri (based on 500 operative cases)," which was published in 1912, detailed his standardization of the radical hysterectomy and formed the basis of ...

    journal_title:Gynecologic oncology

    pub_type: 历史文章,杂志文章


    authors: Swailes AL,Gockley A,Phaëton R,Kesterson JP

    更新日期:2017-04-01 00:00:00

  • Adenocarcinoma of Skene's duct associated with a systemic coagulopathy.

    abstract::Adenocarcinoma of the urethra is a rare neoplasm which is believed to arise from the periurethral ducts. Four such cases have been diagnosed at our institution during the past 25 years and their clinical courses are summarized in this report. In one patient, a disseminated coagulopathy with a predominantly fibrinolyti...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Taylor RN,Lacey CG,Shuman MA

    更新日期:1985-10-01 00:00:00

  • Phase 2 trial of carboplatin plus tamoxifen in platinum-resistant ovarian cancer and primary carcinoma of the peritoneum.

    abstract:OBJECTIVES:A previously reported phase 2 trial suggested substantial clinical activity associated with the combination of a platinum agent and tamoxifen in the treatment of platinum-resistant ovarian cancer. We wished to confirm or refute this observation in a patient population with well-characterized platinum-resista...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章


    authors: Markman M,Webster K,Zanotti K,Peterson G,Kulp B,Belinson J

    更新日期:2004-08-01 00:00:00

  • Aberrant expression and mutations of TGF-beta receptor type II gene in endometrial cancer.

    abstract:OBJECTIVE:Transforming growth factor beta (TGF-beta) is a multifunctional cytokine that strongly inhibits epithelial cell growth. Disabling of TGF-beta signaling is thought to be involved in development of a variety of tumors in which abnormal expression or function of TGF-beta receptor plays critical roles. In the pre...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Sakaguchi J,Kyo S,Kanaya T,Maida Y,Hashimoto M,Nakamura M,Yamada K,Inoue M

    更新日期:2005-09-01 00:00:00

  • Ovarian cancer tumor infiltrating T-regulatory (T(reg)) cells are associated with a metastatic phenotype.

    abstract:OBJECTIVE:The objective of this study was to examine the clinicopathologic correlates of T-regulatory (T(reg)) cell infiltration in serous ovarian cancers and to define gene signatures associated with high T(reg)s. METHODS:Tumor infiltrating T(reg) and cytotoxic T-cells (CTLs) were quantitated in 232 primary serous ov...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Barnett JC,Bean SM,Whitaker RS,Kondoh E,Baba T,Fujii S,Marks JR,Dressman HK,Murphy SK,Berchuck A

    更新日期:2010-03-01 00:00:00

  • High prevalence of genital human papillomavirus type 52 and 58 infection in women attending gynecologic practitioners in South Taiwan.

    abstract:BACKGROUND:We attempted to determine the prevalence of genital human papillomavirus (HPV) infection in women attending gynecologic practitioners in South Taiwan. METHODS:The population included 4383 women aged 16-78 seeking HPV testing at primary gynecologic practitioners regardless of their cervical cytology results....

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Lin H,Ma YY,Moh JS,Ou YC,Shen SY,ChangChien CC

    更新日期:2006-04-01 00:00:00

  • Healthcare resource use and costs associated with cervical, vaginal and vulvar cancers in a large U.S. health plan.

    abstract:OBJECTIVES:To estimate healthcare resource utilization and costs of cervical, vulvar and vaginal cancers in a large U.S. health plan. METHODS:We estimated incremental ambulatory visits, hospitalizations, prescription fills and healthcare costs for cancer cases relative to population controls. Data for cervical (n=2788...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Insinga RP,Ye X,Singhal PK,Carides GW

    更新日期:2008-11-01 00:00:00

  • Low-grade serous carcinoma: new concepts and emerging therapies.

    abstract::For the past several years, all women with epithelial ovarian cancer have been treated identically, whether in a clinical trial or off protocol. Over the past decade, we have come to appreciate the magnitude of the heterogeneity of ovarian cancer. The development of the binary grading system for serous carcinoma was a...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Romero I,Sun CC,Wong KK,Bast RC Jr,Gershenson DM

    更新日期:2013-09-01 00:00:00

  • Vaginal reconstruction at the time of pelvic exenteration: a surgical and psychosexual analysis of techniques.

    abstract:OBJECTIVES:Vaginal reconstruction following pelvic exenteration is an important aspect of the physical and psychological rehabilitation of women after radical surgery for pelvic malignancies. The choice of techniques is vast, and proper patient and surgical selection is important for obtaining satisfactory functional a...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Mirhashemi R,Averette HE,Lambrou N,Penalver MA,Mendez L,Ghurani G,Salom E

    更新日期:2002-10-01 00:00:00

  • Use of CA 125 to monitor patients with ovarian epithelial carcinomas.

    abstract::The CA 125 radioimmunoassay has been increasingly used to monitor the course of patients with ovarian epithelial carcinomas. The purpose of this report is to describe our experience in the use of this assay and to better define its clinical utility. Fifty-one patients had serum CA 125 follow-up during primary chemothe...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Podczaski E,Whitney C,Manetta A,Larson JE,Kirk J,Stevens CW,Lyter J,Mortel R

    更新日期:1989-05-01 00:00:00

  • Effect of recombinant human tumor necrosis factor on A2774 human ovarian cancer cell line: potentiation of mitoxantrone cytotoxicity.

    abstract::Recombinant human tumor necrosis factor (rHuTNF) is a macrophage-secretory protein with antitumor activity. In vivo and in vitro cytotoxicity studies have been carried out to test the effectiveness of rHuTNF alone or in combination with chemotherapeutic agents. We have evaluated the direct cytotoxic effect of rHuTNF o...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Vigani A,Chiara S,Miglietta L,Repetto L,Conte PF,Cimoli G,Morelli L,Billi G,Parodi S,Russo P

    更新日期:1991-04-01 00:00:00

  • MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer.

    abstract:OBJECTIVES:Most ovarian cancer patients are diagnosed at an advanced stage (67%) and prospects for significant improvement in survival reside in early diagnosis. While expression patterns of a recently identified biomarker family, microRNA, appear to be characteristic of tumor type and developmental origin, microRNA pr...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Taylor DD,Gercel-Taylor C

    更新日期:2008-07-01 00:00:00

  • Co-expression patterns of chimeric antigen receptor (CAR)-T cell target antigens in primary and recurrent ovarian cancer.

    abstract:OBJECTIVE:Chimeric antigen receptor (CAR)-T cell strategies ideally target a surface antigen that is exclusively and uniformly expressed by tumors; however, no such antigen is known for high-grade serous ovarian carcinoma (HGSC). A potential solution involves combinatorial antigen targeting with AND or OR logic-gating....

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Banville AC,Wouters MCA,Oberg AL,Goergen KM,Maurer MJ,Milne K,Ashkani J,Field E,Ghesquiere C,Jones SJM,Block MS,Nelson BH

    更新日期:2021-02-01 00:00:00

  • Risk-specific follow-up for endometrial carcinoma patients.

    abstract:OBJECTIVE:To propose a risk-specific follow-up protocol for endometrial carcinoma patients. METHODS:A retrospective cohort of endometrial carcinoma patients was used to identify risk factors for recurrence. Based on a profile of risk factors, women were classified at either low or high risk for recurrence (median foll...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Shumsky AG,Brasher PM,Stuart GC,Nation JG

    更新日期:1997-06-01 00:00:00

  • Primary non-gestational choriocarcinoma of the uterine cervix: a case report.

    abstract:BACKGROUND:Primary non-gestational choriocarcinoma of the female genital tract has been described in the ovaries and is very unusual in other genital sites. CASE:Primary non-gestational uterine cervical choriocarcinoma was diagnosed in a patient, 32, single, without previous sexual contact nor antecedent pregnancy, ad...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Maestá I,Michelin OC,Traiman P,Hokama P,Rudge MV

    更新日期:2005-07-01 00:00:00

  • Distribution of involucrin in normal and pathological human uterine cervix.

    abstract::A study was undertaken to determine the potential value of involucrin immunostaining, a protein synthesized by mature squamous epithelial cells, in distinguishing benign from neoplastic lesions in cervical pathology. A total of 146 cervical biopsies were analyzed using an indirect immunoperoxidase method and polyclona...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Serra V,Ramirez AA,Lara C,Marzo C,Castells A,Bonilla-Musoles F

    更新日期:1990-01-01 00:00:00

  • The inherent cellular radiosensitivity of epithelial ovarian carcinoma.

    abstract::Ovarian carcinomas of similar histology have variable responses to radiation therapy. It has been suggested that inherent cellular resistance to radiation may in part underlie radiotherapy failure. To determine in vitro radiobiological parameters of papillary serous adenocarcinoma of the ovary, we investigated the cel...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Rotmensch J,Schwartz JL,Atcher RW,Grdina DJ,Toohill M,Weichselbaum RW

    更新日期:1989-12-01 00:00:00

  • GRP78 induced by estrogen plays a role in the chemosensitivity of endometrial cancer.

    abstract:OBJECTIVE:Molecular chaperone 78 kDa glucose-regulated protein (GRP78) is a residential protein in the endoplasmic reticulum (ER) that is induced by an unfolded-protein response triggered under many kinds of stress against a cell. GRP78 is also known to act as an anti-apoptotic factor by protecting ER-stress-induced ce...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Luvsandagva B,Nakamura K,Kitahara Y,Aoki H,Murata T,Ikeda S,Minegishi T

    更新日期:2012-07-01 00:00:00

  • A phase II trial of high-dose mitoxantrone, carboplatin, and cyclophosphamide with autologous bone marrow rescue for recurrent epithelial ovarian carcinoma: analysis of risk factors for clinical outcome.

    abstract::Despite high initial response rates to platinum-based chemotherapy, most patients with advanced ovarian carcinoma die of drug-resistant disease. Drug resistance can be overcome in the hematologic malignancies and lymphomas with high-dose therapy and bone marrow transplantation (BMT) when used early, suggesting that th...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章


    authors: Stiff P,Bayer R,Camarda M,Tan S,Dolan J,Potkul R,Loutfi S,Kinch L,Sosman J,Peace D

    更新日期:1995-06-01 00:00:00

  • A clinicopathological analysis of 40 cases of ovarian Sertoli-Leydig cell tumors.

    abstract:OBJECTIVE:To evaluate the clinicopathological features of ovarian Sertoli-Leydig cell tumors (SLCTs) and to explore the reasonable therapy. METHODS:A total of 40 cases of SLCTs were retrospectively reviewed. RESULTS:The incidence of SLCTs was 0.41%, with a median age of 28 years. All tumors were confined to one ovary...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Gui T,Cao D,Shen K,Yang J,Zhang Y,Yu Q,Wan X,Xiang Y,Xiao Y,Guo L

    更新日期:2012-11-01 00:00:00

  • The efficacy of a sonographic morphology index in identifying ovarian cancer: a multi-institutional investigation.

    abstract::Transvaginal sonography (TVS) has been shown to be the most effective means to screen for ovarian cancer. TVS is associated with a high sensitivity and specificity. However, the positive predictive value associated with TVS in the diagnosis of malignancy is low. A morphologic scoring index for use with TVS has been us...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: DePriest PD,Varner E,Powell J,Fried A,Puls L,Higgins R,Shenson D,Kryscio R,Hunter JE,Andrews SJ

    更新日期:1994-11-01 00:00:00

  • Evaluation of EVI1 and EVI1s (Delta324) as potential therapeutic targets in ovarian cancer.

    abstract:PURPOSE:The MDS1 and EVI1 complex locus (MECOM) at 3q26 gives rise to several alternatively spliced transcripts implicated in leukemic oncogenesis. Overexpression of EVI1 in ovarian cancer has led to a proposed oncogenic role. Our objective was to evaluate the therapeutic potential of EVI1 and EVI1s (also known as Delt...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Jazaeri AA,Ferriss JS,Bryant JL,Dalton MS,Dutta A

    更新日期:2010-08-01 00:00:00

  • CHAP II therapy for epithelial ovarian cancers following primary treatment with platinum-based combination chemotherapy.

    abstract::Nineteen patients with stage III or IV epithelial ovarian cancer treated on a prospective randomized protocol with either adriamycin and cis-diamminedichloroplatinum or tamoxifen, adriamycin, and cis-diamminedichloroplatinum received a modified "CHAP II" regimen at the time of clinical progression of disease or the fi...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Schwartz PE,Keating G,Kohorn EI,Chambers JT

    更新日期:1986-11-01 00:00:00

  • Feasibility, safety and clinical outcomes of cardiophrenic lymph node resection in advanced ovarian cancer.

    abstract:OBJECTIVES:Surgical resection of enlarged cardiophrenic lymph nodes (CPLNs) in primary treatment of advanced ovarian cancer has not been widely studied. We report on a cohort of patients undergoing CPLN resection during primary cytoreductive surgery (CRS), examining its feasibility, safety, and potential impact on clin...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Cowan RA,Tseng J,Murthy V,Srivastava R,Long Roche KC,Zivanovic O,Gardner GJ,Chi DS,Park BJ,Sonoda Y

    更新日期:2017-11-01 00:00:00

  • Updates and emerging therapies for rare epithelial ovarian cancers: one size no longer fits all.

    abstract::Epithelial ovarian carcinoma consists of not one, but several, entities. A number of subtypes exist, including high-grade and low-grade serous carcinomas, clear cell, endometrioid carcinoma and mucinous carcinoma. Historically, women with epithelial ovarian cancer have been treated similarly and "lumped" in the same c...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Groen RS,Gershenson DM,Fader AN

    更新日期:2015-02-01 00:00:00