Transcriptome profiling of sulfur-responsive genes in Arabidopsis reveals global effects of sulfur nutrition on multiple metabolic pathways.


:Sulfate is a macronutrient required for cell growth and development. Arabidopsis has two high-affinity sulfate transporters (SULTR1;1 and SULTR1;2) that represent the sulfate uptake activities at the root surface. Sulfur limitation (-S) response relevant to the function of SULTR1;2 was elucidated in this study. We have isolated a novel T-DNA insertion allele defective in the SULTR1;2 sulfate transporter. This mutant, sel1-10, is allelic with the sel1 mutants identified previously in a screen for increased tolerance to selenate, a toxic analog of sulfate (Shibagaki et al., 2002). The abundance of SULTR1;1 mRNA was significantly increased in the sel1-10 mutant; however, this compensatory up-regulation of SULTR1;1 was not sufficient to restore the growth. The sulfate content of the mutant was 10% to 20% of the wild type, suggesting that induction of SULTR1;1 is not fully complementing the function of SULTR1;2 and that SULTR1;2 serves as the major facilitator for the acquisition of sulfate in Arabidopsis roots. Transcriptome analysis of approximately 8,000 Arabidopsis genes in the sel1-10 mutant suggested that dysfunction of the SULTR1;2 transporter can mimic general -S symptoms. Hierarchal clustering of sulfur responsive genes in the wild type and mutant indicated that sulfate uptake, reductive sulfur assimilation, and turnover of secondary sulfur metabolites are activated under -S. The profiles of -S-responsive genes further suggested induction of genes that may alleviate oxidative damage and generation of reactive oxygen species caused by shortage of glutathione.


Plant Physiol


Plant physiology


Maruyama-Nakashita A,Inoue E,Watanabe-Takahashi A,Yamaya T,Takahashi H




Has Abstract


2003-06-01 00:00:00














  • Effects of inorganic phosphate on the light dependent thylakoid energization of intact spinach chloroplasts.

    abstract::The light dependent energization of the thylakoid membrane was analyzed in isolated intact spinach (Spinacia oleracea L.) chloroplasts incubated with different concentrations of inorganic phosphate (Pi). Two independent methods were used: (a) the accumulation of [(14)C]5,5-dimethyl-2,4-oxazolidinedione and [(14)C] met...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Heineke D,Stitt M,Heldt HW

    更新日期:1989-09-01 00:00:00

  • Identification and Quantification of Indole-3-methanol in Etiolated Seedlings of Scots Pine (Pinus sylvestris L.).

    abstract::Combined gas chromatography-mass spectrometry has been used to identify indole-3-methanol in a purified buffer extract from etiolated seedlings of Pinus sylvestris L. Quantitative estimates obtained by high performance liquid chromatography with fluorescence detection, corrected for losses occurring during purificatio...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Sundberg B,Sandberg G,Jensen E

    更新日期:1985-04-01 00:00:00

  • Saturation Kinetics of the Velocity of Stomatal Closing in Response to CO(2).

    abstract::Stomatal closing movements in response to changes from CO(2)-free to CO(2)-containing air were recorded in leaf sections of Zea mays using air flow porometers. The response to CO(2) was fast; the shortest lag between the application of 300 microliters CO(2) per liter of air and the beginning of a stomatal response was...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Raschke K

    更新日期:1972-02-01 00:00:00

  • Beta-Amylases from Alfalfa (Medicago sativa L.) Roots.

    abstract::Amylase was found in high activity (193 international units per milligram protein) in the tap root of alfalfa (Medicago sativa L. cv. Sonora). The activity was separated by gel filtration chromatography into two fractions with molecular weights of 65,700 (heavy amylase) and 41,700 (light amylase). Activity staining of...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Doehlert DC,Duke SH,Anderson L

    更新日期:1982-05-01 00:00:00

  • Induction of curvature in maize roots by calcium or by thigmostimulation: role of the postmitotic isodiametric growth zone.

    abstract::We examined the response of primary roots of maize (Zea mays L. cv Merit) to unilateral application of calcium with particular attention to the site of application, the dependence on growth rate, and possible contributions of thigmotropic stimulation during application. Unilateral application of agar to the root cap ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ishikawa H,Evans ML

    更新日期:1992-01-01 00:00:00

  • ADPglucose Pyrophosphorylase Is Encoded by Different mRNA Transcripts in Leaf and Endosperm of Cereals.

    abstract::Western blots of soluble protein from wheat, rice, and corn showed that ADPglucose pyrophosphorylase subunits have a size of 50 kilodaltons from endosperm tissue and 43 and 46 kilodaltons from leaf. Antisera to ADPglucose pyrophosphorylase precipitated in vitro translation products of 73 and 76 kilodaltons when leaf p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Krishnan HB,Reeves CD,Okita TW

    更新日期:1986-06-01 00:00:00

  • Cytokinin-to-Auxin Ratios and Morphology of Shoots and Tissues Transformed by a Chimeric Isopentenyl Transferase Gene.

    abstract::Tissues transformed with the isopentenyl transferase (ipt) gene cloned from the T-DNA region of the Ti plasmid or with the ipt gene placed under the control of the cauliflower mosaic virus 35S promoter (35S-ipt) were analyzed for auxin and cytokinin. Free and total indole-3-acetic acid (IAA) levels in 35S-ipt-transfor...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Smigocki AC,Owens LD

    更新日期:1989-11-01 00:00:00

  • RNA interference suppression of genes in glycosyl transferase families 43 and 47 in wheat starchy endosperm causes large decreases in arabinoxylan content.

    abstract::The cell walls of wheat (Triticum aestivum) starchy endosperm are dominated by arabinoxylan (AX), accounting for 65% to 70% of the polysaccharide content. Genes within two glycosyl transferase (GT) families, GT43 (IRREGULAR XYLEM9 [IRX9] and IRX14) and GT47 (IRX10), have previously been shown to be involved in the syn...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lovegrove A,Wilkinson MD,Freeman J,Pellny TK,Tosi P,Saulnier L,Shewry PR,Mitchell RA

    更新日期:2013-09-01 00:00:00

  • Rapid regulation of the methylerythritol 4-phosphate pathway during isoprene synthesis.

    abstract::More volatile organic carbon is lost from plants as isoprene than any other molecule. This flux of carbon to the atmosphere affects atmospheric chemistry and can serve as a substrate for ozone production in polluted air. Isoprene synthesis may help leaves cope with heatflecks and active oxygen species. Isoprene syntha...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wolfertz M,Sharkey TD,Boland W,Kühnemann F

    更新日期:2004-08-01 00:00:00

  • Evidence for the Contribution of the Mehler-Peroxidase Reaction in Dissipating Excess Electrons in Drought-Stressed Wheat.

    abstract::Gross O2 evolution and uptake by attached, drought-stressed leaves of wheat (Triticum aestivum) were measured using a 16O2/ 18O2 isotope technique and mass spectrometry. The activity of photosystem II, determined from the rate of 16O2 evolution, is only slightly affected under drought conditions. During drought stress...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Biehler K,Fock H

    更新日期:1996-09-01 00:00:00

  • Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.

    abstract::Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata al...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Grison MS,Kirk P,Brault ML,Wu XN,Schulze WX,Benitez-Alfonso Y,Immel F,Bayer EM

    更新日期:2019-09-01 00:00:00

  • Purification and Characteristics of Sorbitol-6-phosphate Dehydrogenase from Loquat Leaves.

    abstract::To study the role of sorbitol-6-phosphate dehydrogenase in sorbitol synthesis in leaves of Rosaceous plants, properties of the enzyme and its presence in several plants in the family was investigated. The activity of the enzyme, which catalyzes an NADP-dependent oxidation of the substrate to glucose-6-phosphate, was d...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hirai M

    更新日期:1981-02-01 00:00:00

  • Functional genomic analysis supports conservation of function among cellulose synthase-like a gene family members and suggests diverse roles of mannans in plants.

    abstract::Mannan polysaccharides are widespread among plants, where they serve as structural elements in cell walls, as carbohydrate reserves, and potentially perform other important functions. Previous work has demonstrated that members of the cellulose synthase-like A (CslA) family of glycosyltransferases from Arabidopsis (Ar...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Liepman AH,Nairn CJ,Willats WG,Sørensen I,Roberts AW,Keegstra K

    更新日期:2007-04-01 00:00:00

  • The reb1-1 mutation of Arabidopsis. Effect on the structure and localization of galactose-containing cell wall polysaccharides.

    abstract::The Arabidopsis (Arabidopsis thaliana) root epidermal bulger1-1 (reb1-1) mutant (allelic to root hair defective1 [rhd1]) is characterized by a reduced root elongation rate and by bulging of trichoblast cells. The REB1/RHD1 gene belongs to a family of UDP-D-Glucose 4-epimerases involved in the synthesis of D-Galactose ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Nguema-Ona E,Andème-Onzighi C,Aboughe-Angone S,Bardor M,Ishii T,Lerouge P,Driouich A

    更新日期:2006-04-01 00:00:00

  • Early steps in proanthocyanidin biosynthesis in the model legume Medicago truncatula.

    abstract::Oligomeric proanthocyanidins (PAs) composed primarily of epicatechin units accumulate in the seed coats of the model legume Medicago truncatula, reaching maximal levels at around 20 d after pollination. Genes encoding the single Medicago anthocyanidin synthase (ANS; EC and leucoanthocyanidin reductase (LAR...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pang Y,Peel GJ,Wright E,Wang Z,Dixon RA

    更新日期:2007-11-01 00:00:00

  • Identification of a 1-aminocyclopropane-1-carboxylic acid synthase gene linked to the female (F) locus that enhances female sex expression in cucumber.

    abstract::Sex determination in cucumber (Cucumis sativus L.) is controlled largely by three genes: F, m, and a. The F and m loci interact to produce monoecious (M_f_) or gynoecious (M_f_) sex phenotypes. Ethylene and factors that induce ethylene biosynthesis, such as 1-aminocyclopropane-1-carboxylate (ACC) and auxin, also enhan...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Trebitsh T,Staub JE,O'Neill SD

    更新日期:1997-03-01 00:00:00

  • arc6, A Fertile Arabidopsis Mutant with Only Two Mesophyll Cell Chloroplasts.

    abstract::A novel mutant of Arabidopsis thaliana, arc6 (accumulation and replication of chloroplasts), has been isolated from a transfer DNA-mutagenized population of Arabidopsis seedlings. arc6 has the most extreme arc mutant phenotype we have yet described, with only one to three chloroplasts per leaf mesophyll cell compared ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pyke KA,Rutherford SM,Robertson EJ,Leech RM

    更新日期:1994-11-01 00:00:00

  • Comparative freezing patterns in stems of cherry and azalea.

    abstract::Ice formation in stems, as determined by means of an electrophoretic mobility technique, occurs much more rapidly in azalea than in sour cherry. The difference is more marked in the bark than in the wood. Disrupting the structure of the tissues completely eliminates differences in freezing patterns, although gross ana...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dennis FG,Lumis GP,Olien CR

    更新日期:1972-11-01 00:00:00

  • Direct Inhibition of Plant Mitochondrial Respiration by Elevated CO2.

    abstract::Doubling the concentration of atmospheric CO2 often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO2 by 360 [mu]L L-1 above ambient on O2 uptake in isolated mitochondria from soybean (Glycine max L. cv Ransom) cot...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Gonzalez-Meler MA,Ribas-Carbo M,Siedow JN,Drake BG

    更新日期:1996-11-01 00:00:00

  • Flow cytometry of spinach chloroplasts : determination of intactness and lectin-binding properties of the envelope and the thylakoid membranes.

    abstract::Intact spinach (Spinacia oleracea) chloroplasts, thylakoid membranes, and inside-out or right-side-out thylakoid vesicles have been characterized by flow cytometry with respect to forward angle light scatter, right angle light scatter, and chlorophyll fluorescence. Analysis of intact chloroplasts with respect to forwa...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schröder WP,Petit PX

    更新日期:1992-11-01 00:00:00

  • Ectopic expression of an amino acid transporter (VfAAP1) in seeds of Vicia narbonensis and pea increases storage proteins.

    abstract::Storage protein synthesis is dependent on available nitrogen in the seed, which may be controlled by amino acid import via specific transporters. To analyze their rate-limiting role for seed protein synthesis, a Vicia faba amino acid permease, VfAAP1, has been ectopically expressed in pea (Pisum sativum) and Vicia nar...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Rolletschek H,Hosein F,Miranda M,Heim U,Götz KP,Schlereth A,Borisjuk L,Saalbach I,Wobus U,Weber H

    更新日期:2005-04-01 00:00:00

  • Adaptation to CO(2) Level and Changes in the Phosphorylation of Thylakoid Proteins during the Cell Cycle of Chlamydomonas reinhardtii.

    abstract::The photosynthetic performance of synchronously grown Chlamydomonas reinhardtii alternated rhythmically during the cell cycle. The activity of the "CO(2) concentrating mechanism" including the ability to accumulate CO(2) internally and the activity of carbonic anhydrase peaked after 6 to 9 hours of light and reached m...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Marcus Y,Schuster G,Michaels A,Kaplan A

    更新日期:1986-02-01 00:00:00

  • Relation between the Light-Harvesting Chlorophyll a-Protein Complex LHCPa and Photosystem I in the Alga Chlamydobotrys stellata.

    abstract::The light-harvesting chlorophyll protein system of the alga Chlamydobotrys stellata consists of an as yet uncharacterized algal chlorophyll a-protein, called LHCPa, and a common photosystem II-related chlorophyll a/b-protein, called LHCPb (Brandt, Kaiser-Jarry, Wiessner 1982 Biochim Biophys Acta 679: 404-409). For fur...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Brandt P,Zufall E,Wiessner W

    更新日期:1983-01-01 00:00:00

  • Chlorophyll a fluorescence and carbon assimilation in developing leaves of light-grown cucumber.

    abstract::The development of photochemical activity and carbon assimilation in light-grown cucumber (Cucumis sativus L. cv Natsusairaku) leaves was studied to determine the pattern of acquisition and its relationship to leaf growth and expansion. Measurements of chlorophyll a fluorescence showed that leaves acquire photochemica...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Croxdale JG,Omasa K

    更新日期:1990-07-01 00:00:00

  • Electrical Potentials during Gravitropism in Bean Epicotyls.

    abstract::An apparatus for measuring simultaneously the surface electrical potentials along epicotyl was developed to study the largely bending situation by gravity. Potentials increased on the upper side and decreased on the lower side after the horizontal placement. The time course of electrical changes consisted of two compo...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Imagawa K,Toko K,Ezaki S,Hayashi K,Yamafuji K

    更新日期:1991-09-01 00:00:00

  • On the Role of Stored mRNA in Protein Synthesis in Embryonic Axes of Germinating Vigna unguiculata Seeds.

    abstract::Polyadenylated (poly A(+)) RNAs were prepared from both dry and incubated embryonic axes of Vigna unguiculata seeds and were translated by a wheat germ translation system. Analysis with gel electrophoresis and fluorography showed that translation products of poly A(+) RNA from dry embryonic axes were nearly the same a...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Suzuki Y,Minamikawa T

    更新日期:1985-10-01 00:00:00

  • A Photorespiratory Mutant of Chlamydomonas reinhardtii.

    abstract::A mutant strain of Chlamydomonas reinhardtii, designated 18-7F, has been isolated and characterized. 18-7F requires a high CO(2) concentration for photoautrophic growth in spite of the apparent induction of a functional CO(2) concentrating mechanism in air-adapted cells. In 2% O(2) the photosynthetic characteristics o...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Suzuki K,Marek LF,Spalding MH

    更新日期:1990-05-01 00:00:00

  • The NAC Transcription Factors OsNAC20 and OsNAC26 Regulate Starch and Storage Protein Synthesis.

    abstract::Starch and storage proteins determine the weight and quality of cereal grains. Synthesis of these two grain components has been comprehensively investigated, but the transcription factors responsible for their regulation remain largely unknown. In this study, we investigated the roles of NAM, ATAF, and CUC (NAC) trans...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wang J,Chen Z,Zhang Q,Meng S,Wei C

    更新日期:2020-12-01 00:00:00

  • Effects of ethylene, kinetin, and calcium on growth and wall composition of pea epicotyls.

    abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Mondal MH,Nance JF

    更新日期:1975-03-01 00:00:00

  • In VivoP NMR Spectroscopic Studies of Soybean Bradyrhizobium Symbiosis: I. Optimization of Parameters.

    abstract::(31)P NMR spectroscopy was used to study in vivo the symbiotic state established between soybean (Glycine max [L.] Merr. cv Williams) and Bradyrhizobium japonicum (USDA 110 and 138). Different experimental conditions were used to maintain perfused, respiring detached or attached nodules in an NMR magnet. The pH of the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Rolin DB,Boswell RT,Sloger C,Tu SI,Pfeffer PE

    更新日期:1989-04-01 00:00:00