The measurement of cerebral blood flow in the rat.

Abstract:

:Cerebral blood flow (CBF) was determined in the rat under 70% nitrous oxide anesthesia and pentobarbital anesthesia. The application of the Fick principle technique of Kety et al. was modified utilizing 133Xe infused intravenously steadily for 30 seconds, at which time the animal was decapitated and the head frozen in liquid nitrogen. A prior femoral artery to femoral vein shunt was led through a polyethylene catheter of 0.13 ml volume. This catheter passed as a coil in a NaI crystal well-counter with the arterial 133Xe concentration curve recorded by a ratemeter-recorder system. The results of the hemispheric blood flow (HBF) were: under 70% nitrous oxide anesthesia in normocapnia (Paco2 38 mm Hg), 86 +/- 15 ml/100 gm per minute; with hypocapnia (Paco2 20 mm Hg), 40 +/- 5 ml/100 gm per minute; with hypercapnia (Paco2 63 mm Hg), 187 +/- 10 ml/100 gm per minute; and with pentobarbital anesthesia (Paco2 38 mm Hg), 41 +/- 8 ml/100 gm per minute.

journal_name

Stroke

journal_title

Stroke

authors

Matsumoto A,Namon R,Utsunomiya Y,Kogure K,Scheinberg P,Reinmuth OM

doi

10.1161/01.str.6.6.630

subject

Has Abstract

pub_date

1975-11-01 00:00:00

pages

630-7

issue

6

eissn

0039-2499

issn

1524-4628

journal_volume

6

pub_type

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