Abstract:
:The human insulin receptor exists in two isoforms (HIR-A alpha-subunit 719 amino acids and HIR-B alpha-subunit 731 amino acids) which are generated by alternative splicing of a small exon and display distinct patterns of tissue-specific expression. Using the polymerase chain reaction we have recently shown that skeletal muscle of non-diabetic individuals contains predominantly mRNA encoding HIR-A while in skeletal muscle derived from subjects with Type 2 (non-insulin-dependent) diabetes mellitus similar amounts of each mRNA are expressed. We used a polyclonal antibody which discriminates between HIR-A and HIR-B to assess the isoform expression at the protein level. The antibody showed clearly distinct displacement of insulin binding in skeletal muscle membranes of non-diabetic subjects compared to Type 2 diabetic subjects (displacement of specific 125I-insulin binding: 13 non-diabetic subjects 70.0% +/- 14.34, 12 Type 2 diabetic subjects 32.6% +/- 17.45). A control antibody which does not discriminate between both isoforms showed similar displacement of 125I-insulin in membranes of non-diabetic and Type 2 diabetic subjects. These data suggest that the altered expression of receptor isotype mRNA in the skeletal muscle of Type 2 diabetic subjects leads to an altered receptor isoform pattern in the plasma membrane. While skeletal muscle membranes of non-diabetic subjects contain predominantly HIR-A, membranes of Type 2 diabetic subjects show an increased level of HIR-B in addition to HIR-A.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Kellerer M,Sesti G,Seffer E,Obermaier-Kusser B,Pongratz DE,Mosthaf L,Häring HUdoi
10.1007/BF00404072subject
Has Abstractpub_date
1993-07-01 00:00:00pages
628-32issue
7eissn
0012-186Xissn
1432-0428journal_volume
36pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract:AIMS/HYPOTHESIS:The mechanisms by which transcription factor 7-like 2 (TCF7L2) regulates the pathways that are important in the pathogenesis of type 2 diabetes are unknown. We therefore examined the role of TCF7L2 in hepatic glucose production (HGP) in vitro and characterised the whole-genome chromatin occupancy of TCF...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-011-2289-z
更新日期:2011-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Intraportal human islet cell grafts do not consistently and sustainably induce insulin-independency in type 1 diabetic patients. The reasons for losses in donor cells are difficult to assess in patients. This study in streptozotocin-diabetic nude rats examines whether outcome is better in an extra-hepat...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-010-1721-0
更新日期:2010-08-01 00:00:00
abstract::In a population-based survey of 2,930 subjects, prevalence rates for obesity, Type 2 (non-insulin-dependent) diabetes mellitus, impaired glucose tolerance, hypertension, hypertriglyceridaemia, and hypercholesterolaemia were 54.3, 9.3, 11.1, 9.8, 10.3 and 9.2%, respectively. The prevalence, however, of each of these co...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00403180
更新日期:1991-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:Numerous adaptations of the maternal immune system are necessary during pregnancy to maintain immunological tolerance to the semi-allogeneic fetus. Several complications of pregnancy have been associated with dysregulation of these adaptive mechanisms. While gestational diabetes mellitus (GDM) has been ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-018-4615-1
更新日期:2018-07-01 00:00:00
abstract::The adrenergic regulation of adipose tissue lipolysis in response to insulin-induced hypoglycaemia (intravenous infusion of soluble insulin 0.10 IU.kg body weight-1.h-1 until the arterial plasma glucose fell below 2.8 mmol/l) was investigated directly in vivo in 11 insulin-dependent diabetic (IDDM) patients and 12 con...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050519
更新日期:1996-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:Glomerular matrix accumulation is a hallmark of diabetic nephropathy. Recent data have linked the serine/threonine kinase protein kinase B (Akt) to matrix modulation. Here, we studied its role in high glucose-induced collagen elaboration by mesangial cells. METHODS:Primary rat mesangial cells were trea...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-007-0721-1
更新日期:2007-09-01 00:00:00
abstract::The role of islet amyloidosis in the onset and progression of Type 2 diabetes remains obscure. Islet amyloid polypeptide is a 37 amino-acid, beta-cell peptide which is co-stored and co-released with insulin. Human islet amyloid polypeptide refolds to a beta-conformation and oligomerises to form insoluble fibrils; prol...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s00125-003-1304-4
更新日期:2004-02-01 00:00:00
abstract::Ciglitazone is orally active in preventing and reversing the hyperglycaemic syndrome in C57BL/6J-ob/ob mice and it is only mildly and transiently hypoglycaemic in lean littermates (C57BL/6J-+/?). Its effect on glucose disposal in vivo was estimated by injecting glucose-6-3H/14C and following the specific activity of r...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00284462
更新日期:1983-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Neuronal dorsal root ganglion (DRG) cells seem to be vulnerable in diabetes. The aim of this study was to determine whether the p75 neurotrophin receptor stimulates perikaryal shrinkage and neuronal death, and further accelerates neuronal DRG cell loss after axotomy in a mouse model of diabetes. METHOD...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-004-1504-6
更新日期:2004-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Sympathetic nerves influence islet hormone levels in the circulation. Insights into islet sympathetic innervation and its remodelling in diabetes may impact future therapeutics. However, standard immunohistochemistry and microtome-based microscopy cannot provide an integral view of the islet neurovascul...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2699-6
更新日期:2012-12-01 00:00:00
abstract::The metabolic and hormonal effects of stable hyperglycaemia (10-12 mmol/l) have been examined in five insulin-dependent diabetics and compared with the results of 8 h (1200 to 2000 h) normoglycaemic (5-6 mmol/l) clamping. Glucose levels were maintained using a glucose controlled insulin infusion system. Mean blood lac...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00262012
更新日期:1981-02-01 00:00:00
abstract::Stroke incidence, case fatality and mortality in diabetic patients were compared to non-diabetic subjects in a 35-74-year-old population in northern Sweden (target population 241,000). During an 8-year period, 1,544 stroke events in diabetic patients and 4,826 events in non-diabetic subjects were recorded. The crude i...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00402176
更新日期:1995-09-01 00:00:00
abstract::Oxidative stress has long been considered a key driving factor of many obesity-related health problems. However, recent work by Merry, Tran et al (Diabetologia DOI 10.1007/s00125-016-4084-3 ) challenges this idea with an interesting study using a hepatocyte-specific Gpx1-knockout (HGKO) mouse. GPX1 is an important det...
journal_title:Diabetologia
pub_type: 评论,社论
doi:10.1007/s00125-016-4117-y
更新日期:2016-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this study was to examine human enteroviruses (HEVs) and other intestinal viruses derived from children who participated in the Babydiet intervention study and to analyse the findings according to the appearance of islet autoantibodies, dietary intervention, maternal type 1 diabetes and clini...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/s00125-011-2305-3
更新日期:2011-12-01 00:00:00
abstract::Glucose-induced cyclic (3H) AMP accumulation, insulin secretory responses and the metabolism of glucose were studied in pancreatic islets from Acomys cahirinus. 27.7 mmol/l of glucose stimulated neither islet cyclic (3H) AMP accumulation nor insulin release during the first 5 min of incubation. Stimulation by glucose ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00423150
更新日期:1979-01-01 00:00:00
abstract::Immunoelectrophoresis of glomerular basement membrane antigens in the urine of 20 Type 1 (insulin-dependent) diabetic and 10 healthy children was performed. In 10 of the diabetic children, there was altered alpha-1-mobility, while the other diabetic and normal children showed alpha-2-mobility. After incubation with gl...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00254295
更新日期:1982-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:We assessed the effects of vildagliptin, a novel dipeptidyl peptidase IV inhibitor, on postprandial lipid and lipoprotein metabolism in patients with type 2 diabetes. SUBJECTS, MATERIALS AND METHODS:This was a single-centre, randomised, double-blind study in drug-naive patients with type 2 diabetes. Pa...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-006-0340-2
更新日期:2006-09-01 00:00:00
abstract::Overall D-glucose metabolism and 3-0-methylglucose transport were measured in the perfused heart preparation of lean and genetically obese (fa/fa) rats. Absolute values of basal and insulin-stimulated glucose metabolism were decreased in hearts of 15-week-old obese rats when compared to lean age-matched controls. Basa...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00284464
更新日期:1983-12-01 00:00:00
abstract::Islet allografts in insulin-dependent diabetic (IDDM) patients exhibit variable survival lengths and low rates of insulin-independence despite treatment with anti-T-cell antibodies and maintenance immunosuppression. Use of poorly characterized freshly isolated preparations makes it difficult to determine whether failu...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1007/s001250050929
更新日期:1998-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Semaglutide is a glucagon-like peptide-1 analogue in development for the treatment of type 2 diabetes. Its effects on first- and second-phase insulin secretion and other measures of beta cell function and glycaemic control were assessed. METHODS:In this single-centre, double-blind, placebo-controlled, ...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-017-4289-0
更新日期:2017-08-01 00:00:00
abstract::In a prospective study concerning the pathogenesis of impaired glucose tolerance and Type 2 (non-insulin-dependent) diabetes mellitus, 346 subjects with no clinical history of diabetes were given a standard 75 g oral glucose tolerance test. The expected positive associations between 120-min plasma glucose concentratio...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00403292
更新日期:1991-07-01 00:00:00
abstract::The role of B lymphocytes in the pathogenesis of type 1 diabetes in humans is not entirely evident. These cells are presumed to be important, but this assumption is largely based on animal models of autoimmune diabetes, where compelling evidence for the contribution of both B lymphocytes and insulin-specific autoantib...
journal_title:Diabetologia
pub_type: 评论,社论
doi:10.1007/s00125-017-4284-5
更新日期:2017-07-01 00:00:00
abstract::The effect of endogenous hyperinsulinaemia, produced by syngeneic transplantation of rat insulinoma at an extrapancreatic site, on pancreatic insulin and pancreatic polypeptide has been examined by radioimmunoassay and immunohistochemistry. Twenty days after subcutaneous transplantation, tumour-bearing rats exhibited ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00273864
更新日期:1985-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:We evaluated the secular trend of glycaemic control in individuals with type 2 diabetes in developing countries, where data are limited. METHODS:The International Diabetes Management Practices Study provides real-world evidence of patient profiles and diabetes care practices in developing countries in ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-019-05078-3
更新日期:2020-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Mutations in the HNF-1 alpha gene result in maturity-onset diabetes of the young (MODY); an early-onset, dominantly inherited form of diabetes caused by pancreatic beta-cell dysfunction. Splice site mutations represent approximately 10% of reported HNF-1 alpha mutations. No studies to date have investig...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-002-0919-1
更新日期:2002-10-01 00:00:00
abstract::Data are discussed which demonstrate that insulin plays an important role in sodium metabolism. The primary action of insulin on sodium balance is exerted on the kidney. Increases in plasma insulin concentration within the physiological range stimulate sodium reabsorption by the distal nephron segments and this effect...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/BF00252649
更新日期:1981-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:It is thought that enterovirus infections initiate or facilitate the pathogenetic processes leading to type 1 diabetes. Exposure of cultured human islets to cytolytic enterovirus strains kills beta cells after a protracted period, suggesting a role for secondary virus-induced factors such as cytokines. ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1839-7
更新日期:2005-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hIPSCs) offer unique opportunities for regenerative medicine and for the study of mammalian development. However, developing methods to differentiate hESCs/hIPSCs into specific cell types following a natural pathway of developm...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2687-x
更新日期:2012-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Maturity onset diabetes of the young type 3 (MODY3) is a monogenic form of diabetes mellitus caused by mutations in the gene encoding for hepatocyte nuclear factor 1 alpha, HNF1 alpha. In this study we have examined the in vivo and in vitro effects of a mutation (L107I) outside the DNA binding and dimer...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-002-0977-4
更新日期:2002-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Perinatal exposure to bisphenol A (BPA), a widely distributed environmental endocrine disruptor, is associated with insulin resistance and diabetes in offspring. The underlying molecular mechanisms could involve epigenetics, as adverse effects induced by environmental exposure in early life are suggeste...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-013-2944-7
更新日期:2013-09-01 00:00:00