Abstract:
:Expression of phosphatidylserine (PS) on the surface of both macrophages and their apoptotic targets is required for efficient phagocytosis. Monocytes, the precursors of macrophages, do not express PS on their surface and do not efficiently phagocytose apoptotic cells. We report here that PS appears on the surface of both human monocytic U937 cells and primary human monocytes as they differentiate in culture and acquire the ability to phagocytose apoptotic thymocytes. Phagocytosis was blocked by pretreating either the apoptotic target or the phagocyte with annexin V to mask PS and was CD14-dependent. Expression of PS, like other events characteristic of differentiating monocytes such as Mac-1 expression, was independent of the agent used to induce differentiation and was insensitive to the addition of caspase inhibitors. These results demonstrate that PS is expressed on monocytes as part of their differentiation program and is independent of apoptosis.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Callahan MK,Halleck MS,Krahling S,Henderson AJ,Williamson P,Schlegel RAdoi
10.1189/jlb.0902433subject
Has Abstractpub_date
2003-11-01 00:00:00pages
846-56issue
5eissn
0741-5400issn
1938-3673pii
jlb.0902433journal_volume
74pub_type
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