Abstract:
:cDNA cloning revealed the presence of two related but distinct types of human interleukin-8 (IL-8) receptors, type I (type A) and type II (type B). By immunizing rabbits with glutathione-S-transferase fused with the NH2-terminal domain of each type of IL-8 receptor, we prepared polyclonal antibodies that specifically recognized the NH2-terminal domain of each type of IL-8 receptor. Immunofluorescence analysis of human peripheral blood leukocytes demonstrated that mature granulocytes except eosinophils express both types of IL-8 receptors. A majority of monocytes and CD16+ natural killer (NK) cells in peripheral blood were stained with both antibodies, whereas CD3+ T or CD20+ B lymphocytes in peripheral blood or CD34+ cells in cord blood were not stained with either antibody. These results suggest that both types of human IL-8 receptors were coordinately and selectively expressed in mature granulocytes, monocytes, and CD16+ NK cells.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Morohashi H,Miyawaki T,Nomura H,Kuno K,Murakami S,Matsushima K,Mukaida Ndoi
10.1002/jlb.57.1.180subject
Has Abstractpub_date
1995-01-01 00:00:00pages
180-7issue
1eissn
0741-5400issn
1938-3673journal_volume
57pub_type
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