Abstract：
:Sera from Brucella-infected bovines were analyzed by immunoblotting by using sonicated cell extracts of B. melitensis or B. abortus and a competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies against outer membrane proteins (OMPs) with molecular masses of 10, 16.5, 19, 25 to 27, 36 to 38, and 89 kDa. Antibody responses against OMPs were compared with antibody responses against smooth lipopolysaccharide. Immunoblot analysis indicated that the antibody response in infected animals was largely different from one animal to another. The antigens of concern were OMPs with molecular masses of 10, 16.5, 19, 25 to 27, 36 to 38, and 89 kDa and other proteins with molecular masses of between 40 and 80 kDa. According to the specificity of the competitive ELISA, OMPs useful for the detection of infected animals are the OMPs of 10, 16.5, 19, 25 to 27, and 36 to 38 kDa. A competitive ELISA with the anti-89 kDa monoclonal antibody was not specific. Results of the competitive ELISA confirmed the individual variability of the humoral immune response against OMPs. It therefore seems that a combination of several protein antigens is necessary for the development of an immunoassay with a sensitivity comparable to that of the smooth lipopolysaccharide ELISA.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Cloeckaert A,Kerkhofs P,Limet JNdoi
10.1128/JCM.30.12.3168-3174.1992subject
Has Abstractpub_date
1992-12-01 00:00:00pages
3168-74issue
12eissn
0095-1137issn
1098-660Xjournal_volume
30pub_type
杂志文章abstract：:Ninety-eight isolates of Cryptococcus neoformans were collected from 30 patients at the University of Iowa Hospitals and Clinics from December 1987 through December 1994. The susceptibility of each isolate was determined against fluconazole, itraconazole, amphotericin B, and flucytosine. Of the 98 isolates, 53 were re...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.36.12.3653-3656.1998
更新日期：1998-12-01 00:00:00
abstract：:The development and evaluation of a new selective medium (colistin-oxolinic acid-blood agar) for streptococci is described. Streptococci of medical and veterinary importance grew well on the medium. Gram-negative organisms, staphylococci. Bacillus spp., and coryneforms are all inhibited. It was concluded that the medi...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.19.1.4-7.1984
更新日期：1984-01-01 00:00:00
abstract：:A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the cultu...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00333-13
更新日期：2013-05-01 00:00:00
abstract：:Gemella morbillorum and Gemella haemolysans are opportunistic pathogens which cause endocarditis and other severe infections. We report on three patients with endocarditis, one with endocarditis caused by G. haemolysans and two with endocarditis caused by G. morbillorum. The paucity of reports concerning these bacteri...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章,评审
doi：10.1128/JCM.36.4.866-871.1998
更新日期：1998-04-01 00:00:00
abstract：:Direct enzyme-linked immunosorbent assay methods offer several advantages in assessing past or recent exposure to cytomegalovirus (CMV) infection, but there persist many pitfalls in the use of these methods for determining specific immunoglobulin M (IgM). The efficiency of absorption of sera by IgG-coated latex beads,...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.23.3.576-581.1986
更新日期：1986-03-01 00:00:00
abstract：:An in-house enzyme-linked immunosorbent assay (ELISA) for measurement of Helicobacter pylori-specific immunoglobulin G (IgG) and IgA in saliva was evaluated by comparison with histopathologic (Giemsa staining) and biochemical (urease quick test) examination of gastric biopsy specimens obtained from 112 children referr...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.35.12.3358-3360.1997
更新日期：1997-12-01 00:00:00
abstract：:Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory powe...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.35.1.325-327.1997
更新日期：1997-01-01 00:00:00
abstract：:We used the slide agglutination test to determine the serologic relationships of 22 Legionella spp. representing 33 serogroups. Antisera prepared against 14 of the Legionella spp. contained cross-reactive antibodies (1+ or greater) at their working dilutions. Numerous cross-reactions were observed for the blue-white f...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.21.5.779-782.1985
更新日期：1985-05-01 00:00:00
abstract：:Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.26.9.1867-1868.1988
更新日期：1988-09-01 00:00:00
abstract：:The diagnosis of congenital syphilis in apparently healthy infants continues to be problematic. Immunoglobulin M antibodies specific for a subset of Treponema pallidum antigens have been detected by Western blotting (immunoblotting). In the present study we investigated the sensitivity and specificity of this method. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.32.3.629-633.1994
更新日期：1994-03-01 00:00:00
abstract：:The epidemiological study of several multidrug-resistant Enterobacteriaceae isolated from five patients demonstrated in vivo dissemination of a 100-kb plasmid encoding the extended-spectrum beta-lactamase TEM-24 from a clonal strain of Enterobacter aerogenes to different strains of Klebsiella pneumoniae, Escherichia c...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.39.5.1985-1988.2001
更新日期：2001-05-01 00:00:00
abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.28.11.2456-2461.1990
更新日期：1990-11-01 00:00:00
abstract：:A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.30.10.2620-2624.1992
更新日期：1992-10-01 00:00:00
abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.42.2.847-849.2004
更新日期：2004-02-01 00:00:00
abstract：:We used an enzyme-linked immunosorbent assay (ELISA) of immunoglobulin G avidity to determine the dengue immune status of 105 pairs of serum samples from patients infected with dengue virus. This study shows that a simple avidity test, for which only one acute-phase serum sample is required, is potentially more useful...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.43.11.5784-5786.2005
更新日期：2005-11-01 00:00:00
abstract：:We used simulated blood cultures inoculated with clinical isolates of Mycoplasma hominis to determine whether liquid media of the BacT/ALERT (Organon Teknika, Durham, N.C.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. Viability of clinical...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.39.12.4328-4331.2001
更新日期：2001-12-01 00:00:00
abstract：:The human immunodeficiency virus (HIV) was isolated from the blood of 192 of 410 seropositive individuals. Original isolations were made in peripheral blood mononuclear cell (PBMC) cultures, and only one-fifth of the HIV isolates could be adapted to replicate in continuous T-cell lines. Of the 192 HIV isolates, 42 had...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.8.1411-1415.1987
更新日期：1987-08-01 00:00:00
abstract：:Respiratory tract specimens from patients with cystic fibrosis (CF) require unique processing by clinical microbiology laboratories to ensure detection of all potential pathogens. The present study sought to determine the compliance of microbiology laboratories in the United States with recently published recommendati...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.44.4.1547-1549.2006
更新日期：2006-04-01 00:00:00
abstract：:A rapid and simple method for extracting specific cell-free antigens of Coccidioides immitis and Histoplasma capsulatum from agar slant cultures was developed. The extracts were analyzed in immunodiffusion tests for the presence of C. immitis or H. capsulatum specific antigens. These extracts were compared with cultur...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:A 13-year-old patient developed severe shock due to administration of a Yersinia enterocolitica-contaminated red blood cell concentrate. Y. enterocolitica (serotype O:9, biotype II) was cultivated from the residual blood in the blood bag and from a stool sample of the blood donor. In the donor's plasma immunoglobulin ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.38.7.2788-2790.2000
更新日期：2000-07-01 00:00:00
abstract：:To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day in...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.06815-11
更新日期：2012-06-01 00:00:00
abstract：:The predominant Helicobacter pylori strains circulating among geographic locations differ in regard to genomic structure. The association of the cagA-positive, vacA s1 genotypes with peptic ulcer disease (PUD) and gastric cancer was reported in Western countries but not in East Asian countries. Strains from Western co...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.42.4.1648-1651.2004
更新日期：2004-04-01 00:00:00
abstract：:In comparative studies, Hemo-De (PMP Medical Industries, Inc., Irving, Tex.) was found to be a suitable replacement for ethyl acetate in the Formalin-ethyl acetate concentration technique. With essentially equivalent recovery rates for both procedures, the Formalin-Hemo-De concentration technique is considered to be t...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.2.425-426.1987
更新日期：1987-02-01 00:00:00
abstract：:The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints since 2010 for bacteria that grow aerobically. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the Enterobacteriaceae and Pseudomonas aeruginosa, daptomycin breakpoints for Enterococcus...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章,评审
doi：10.1128/JCM.00203-19
更新日期：2019-05-24 00:00:00
abstract：:Ureaplasma urealyticum is a commensal organism of the lower genital tract of females, but in a subpopulation of individuals, it can invade the upper genital tract. It is a significant cause of chorioamnionitis and neonatal morbidity and mortality. There are 14 recognized serovars of U. urealyticum; these can be divide...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.32.6.1464-1469.1994
更新日期：1994-06-01 00:00:00
abstract：:A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:Cryptococcus gattii emerged in North America in 1999 as a human and veterinary pathogen on Vancouver Island, British Columbia. The emergent subtype, VGIIa, and the closely related subtype VGIIb can now be found in the United States in Washington, Oregon, and California. We performed multilocus sequence typing and anti...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01505-09
更新日期：2010-02-01 00:00:00
abstract：:We describe two rapid, simple, and reliable procedures for routine purification of hepatitis B virus (HBV) DNA from serum. HBV DNA could be purified from 24 serum samples in 1.5 to 2 h and was recovered in the initial reaction vessel. Both procedures have in common that HBV DNA is complexed with silica particles in th...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.29.9.1804-1811.1991
更新日期：1991-09-01 00:00:00
abstract：:Rapid detection and accurate identification of methicillin-resistant staphylococci are critical for the effective management of infections caused by these organisms. We describe a multiplex PCR-based assay for the direct detection of methicillin-resistant staphylococci from blood culture bottles (BacT/Alert; Organon-T...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.40.8.2786-2790.2002
更新日期：2002-08-01 00:00:00
abstract：:Evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. Bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. A twin microcalorimete...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
