Novel immunohistochemical localization of 28,000 molecular-weight (Mr) calcium binding protein (calbindin-D28k) in enterochromaffin cells of the human appendix and neuroendocrine tumors (carcinoids and small-cell carcinomas) of the midgut and foregut.

Abstract:

:Calbindin-D28k is a highly conserved 28,000 (dalton) molecular-weight (Mr) calcium binding protein with broad tissue distribution, yet cell-type-specific expression predominantly in subpopulations of central and peripheral nervous system neurons, distal tubular cells of the kidney, and enteric neuroendocrine cells. A polyclonal antiserum against rat renal calbindin-D28k and a monoclonal antibody to calbindin-D28k purified from chicken intestine (clone CL-300) were used for immunohistochemical evaluation of formalin-fixed, paraffin-embedded tissues from multiple areas of the human small and large intestines and 93 primary neoplasms of the gastrointestinal tract (foregut, midgut, and hindgut derivatives) and the lung (foregut derivative). Calbindin-D28k immunostaining was obtained in a minority of enterochromaffin (neuroendocrine) cells, predominantly of the appendix and small intestine, as well as in autonomic neurons of the neural plexuses. Focal cytoplasmic Golgi-type staining was obtained with monoclonal antibody CL-300 in the appendiceal surface epithelium and dendritic macrophages confined to the appendiceal lymphoid follicles. Epithelial progenitor cells in enteric crypts and absorptive, goblet, and Paneth cells were calbindin-D28k negative, while no immunoreactivity was demonstrated in the mucosae of the colon and rectum. Calbindin-D28k staining was consistently detected in subpopulations of neuroendocrine phenotypes in midgut (appendiceal/ileal) and foregut (bronchial) carcinoids and small-cell carcinomas, but was absent in adenocarcinomas, squamous cell carcinomas, leiomyomas/leiomyosarcomas, schwannomas, and lymphomas. Our observations suggest that calbindin-D28k is a novel adjuvant neuroendocrine marker that is potentially useful in diagnostic tumor immunohistochemistry.

journal_name

Arch Pathol Lab Med

authors

Katsetos CD,Jami MM,Krishna L,Jackson R,Patchefsky AS,Cooper HS

subject

Has Abstract

pub_date

1994-06-01 00:00:00

pages

633-9

issue

6

eissn

0003-9985

issn

1543-2165

journal_volume

118

pub_type

杂志文章
  • Black cartilage after therapy with levodopa and methyldopa.

    abstract::A study was undertaken of 14 autopsy cases with pigmented rib cartilage. Twelve of these patients had been treated with levodopa because of Parkinson's disease for at least 6 years, and two had been treated with methyldopa because of essential hypertension for 19 years. Thirty-two percent of the autopsy cases of Parki...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Rausing A,Rosén U

    更新日期:1994-05-01 00:00:00

  • Hyperchromatic crowded groups in cervical cytology--differing appearances and interpretations in conventional and ThinPrep preparations: a study from the College of American Pathologists Interlaboratory Comparison Program in Cervicovaginal Cytology.

    abstract:CONTEXT:The practice of gynecologic cytology requires that high-grade squamous intraepithelial lesion (HSIL) be precisely recognized. In this regard, hyperchromatic crowded groups are known to be difficult to classify in conventional gynecologic cytology, but whether this is true in ThinPrep specimens is uncertain. OB...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/1543-2165(2006)130[332:HCGICC]2.0.CO;2

    authors: Renshaw AA,Mody DR,Wang E,Haja J,Colgan TJ,Cytopathology Resource Committee, College of American Pathologists.

    更新日期:2006-03-01 00:00:00

  • Estrogen receptor expression in papillary urothelial carcinoma of the bladder and ovarian transitional cell carcinoma.

    abstract:CONTEXT:Relatively little is known about estrogen receptor (ER) expression in papillary urothelial carcinoma (PUC) of the bladder. Greater understanding of this feature of PUCs could aid with the treatment and identification of the origin of metastases, particularly with relation to the morphologically similar entity o...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/1543-2165(2005)129<194:EREIPU>2.0.CO;2

    authors: Croft PR,Lathrop SL,Feddersen RM,Joste NE

    更新日期:2005-02-01 00:00:00

  • Preventing platelet-derived microparticle formation--and possible side effects-with prestorage leukofiltration of whole blood.

    abstract:CONTEXT:Platelet-derived microparticles (PDMPs) probably function in hemostasis, thrombosis, inflammation, and transfusion-related immunomodulation. OBJECTIVE:To compare PDMP levels of leukocyte-filtered and unfiltered whole blood during storage. DESIGN:Ten whole blood donations were collected and processed. Half of ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/1543-2165-134.5.771

    authors: Sugawara A,Nollet KE,Yajima K,Saito S,Ohto H

    更新日期:2010-05-01 00:00:00

  • Protocol for the examination of specimens from patients with carcinoma of the ampulla of Vater: a basis for checklists. Cancer Committee, College of American Pathologists.

    abstract::A protocol for the pathologic examination and reporting of specimens from patients with carcinoma of the ampulla of Vater has been developed by the Cancer Committee of the College of American Pathologists. The protocol incorporates all basic pathology data of diagnostic and prognostic significance appropriate for the ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Compton CC

    更新日期:1997-07-01 00:00:00

  • Concurrent spindle cell peripheral pulmonary carcinoid tumor and Merkel cell tumor of the skin.

    abstract::To our knowledge this is the first reported case of concurrent Merkel cell (trabecular carcinoma) tumor and peripheral pulmonary carcinoid tumor. Both tumors are considered to neuroendocrine in nature. The significance of concurrent neuroendocrine tumors is described with respect to multiple endocrine neoplasia syndro...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Nelson EL,Houghton DC

    更新日期:1990-04-01 00:00:00

  • Overlap index. An alternative to sensitivity and specificity in comparing the Utility of a laboratory test.

    abstract::The utility of a laboratory test for differential diagnosis depends on the degree that test results for different disease states overlap. I developed a new overlap index that ranges from zero, for an ideal test with no overlap, to one if the median test result is the same for the two disease states. The index does not...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Hartz AJ

    更新日期:1984-01-01 00:00:00

  • The probability of obtaining compatible blood from related directed donors.

    abstract::Patients with alloantibodies may request directed blood donations from their relatives despite the lack of any scientific data suggesting that blood from these donors is safer. We have derived formulas that are applicable to any ethnic group for calculating the probability that a given class of relatives will have com...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Kanter MH,Hodge SE

    更新日期:1990-10-01 00:00:00

  • Causes and relevance of unsatisfactory and satisfactory but limited smears of liquid-based compared with conventional cervical cytology.

    abstract:CONTEXT:Recent randomized controlled trials have shown a significant decrease in unsatisfactory rates for liquid-based cytology (LBC) compared with conventional Papanicolaou test (CP). The underlying causes and relevance of unsatisfactory results for LBC and CP have never been compared within the setting of a randomize...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.5858/arpa.2011-0113-OA

    authors: Siebers AG,Klinkhamer PJ,Vedder JE,Arbyn M,Bulten J

    更新日期:2012-01-01 00:00:00

  • Diabetic neuroarthropathy (Charcot's joint).

    abstract::The pathologic aspects of an uncommon complication of diabetes mellitus, destructive arthropathy (Charcot's joint), were studied. The peripheral nerve demyelination resulting in clinically demonstrable deficits in perception of pain and vibration and in reflexes in the lower extremities reinforces the neurogenic role ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Raju UB,Fine G,Partamian JO

    更新日期:1982-07-01 00:00:00

  • Angiokeratoma of the clitoris.

    abstract::A 25-year-old woman, multigravida, presented with a dark ulcerated tumor of the clitoris. Histologic examination demonstrated an angiokeratoma, a benign telangiectatic vascular tumor. To our knowledge, this is the first report of angiokeratoma of the clitoris and only the fifth describing vascular tumor of this organ....

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: McNeely TB

    更新日期:1992-08-01 00:00:00

  • Pseudomyogenic Hemangioendothelioma (Epithelioid Sarcoma-Like Hemangioendothelioma).

    abstract::Pseudomyogenic hemangioendothelioma (PMH) is a rare vascular tumor that presents more frequently in young adults and has a male predominance. It is usually located in the superficial or deep soft tissues of the extremities, but concurrent bone involvement can be present. In approximately two-thirds of patients this di...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.5858/arpa.2018-0395-RS

    authors: Caballero GA,Roitman PD

    更新日期:2020-04-01 00:00:00

  • Quality control of test systems waived by the Clinical Laboratory Improvement Amendments of 1988. Perceptions and practices.

    abstract:CONTEXT:Recent advances in laboratory testing technology have resulted in a rapidly increasing number of test systems targeted for physician office, point-of-care, and home health care settings. With enhanced error detection mechanisms and unitized reagents, these new systems simplify the testing process and the assess...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/0003-9985(2000)124<1122:QCOTSW>2.0.CO;2

    authors: LaBeau KM,Simon M,Steindel SJ

    更新日期:2000-08-01 00:00:00

  • Origins of human atherosclerotic plaques. The role of altered gene expression.

    abstract::The dramatic rise and equally dramatic fall in the mortality from coronary heart disease in the 20th century is only partly explained. This article reviews the development of our ideas concerning possible pathways other than lipids that might play a role in the development of human atherosclerosis alone or in combinat...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,评审

    doi:

    authors: Benditt EP

    更新日期:1988-10-01 00:00:00

  • Polypoid adenosquamous carcinoma of the esophagus with prominent spindle cells. Report of a case with immunohistochemical and ultrastructural studies.

    abstract::We report a case of spindle-cell adenosquamous carcinoma of the esophagus. This represents the third case in the worldwide literature in which an esophageal polypoid tumor contains adenosquamous carcinoma rather than the usual squamous cell carcinoma in addition to a prominent spindle-cell element. The spindle-cell co...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,评审

    doi:

    authors: Orsatti G,Corvalan AH,Sakurai H,Choi HS

    更新日期:1993-05-01 00:00:00

  • A unique method for mutation analysis of tumor suppressor genes in colorectal carcinomas using a crypt isolation technique.

    abstract:BACKGROUND:Contamination of nontumor tissue makes genetic analysis difficult. For this reason, it is important to obtain pure tumor tissue to ensure accurate genetic analysis. OBJECTIVE:To accurately assess the incidence of mutation of tumor suppressor genes (p53: exon 5-8; APC: mutated cluster region; NF-2 gene: all ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/0003-9985(2000)124<0382:AUMFMA>2.0.CO;2

    authors: Sugai T,Habano W,Nakamura Si,Uesugi N,Sasou S,Itoh C

    更新日期:2000-03-01 00:00:00

  • An in-depth look at Krukenberg tumor: an overview.

    abstract::Krukenberg tumor is an uncommon metastatic tumor of the ovary. This article provides an overview of the major pathologic manifestations of Krukenberg tumor, patient characteristics, clinical and laboratory features of the disease, prognostic factors, and current knowledge about its pathogenesis. Pathologists have to b...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1043/1543-2165(2006)130[1725:AILAKT]2.0.CO;2

    authors: Al-Agha OM,Nicastri AD

    更新日期:2006-11-01 00:00:00

  • Malignant Mesothelioma and Its Non-Asbestos Causes.

    abstract:CONTEXT:- Although many mesotheliomas are related to asbestos exposure, not all are, and there is increasing information on other causes of mesothelioma. OBJECTIVE:- To provide a review of non-asbestos causes for malignant mesothelioma. DATA SOURCES:- Review of relevant published literature via PubMed and other searc...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.5858/arpa.2017-0365-RA

    authors: Attanoos RL,Churg A,Galateau-Salle F,Gibbs AR,Roggli VL

    更新日期:2018-06-01 00:00:00

  • Invasive secretory (juvenile) carcinoma arising in ectopic breast tissue of the axilla.

    abstract::Mammary carcinoma arising in ectopic breast tissue is an uncommon occurrence. Most reported cases have involved ductal carcinoma, but other types, such as medullary, papillary, and lobular carcinomas, have been described. For pathologists, the diagnosis of mammary carcinoma arising in ectopic breast tissue can be diff...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/0003-9985(2001)125<1372:ISJCAI>2.0.CO;2

    authors: Shin SJ,Sheikh FS,Allenby PA,Rosen PP

    更新日期:2001-10-01 00:00:00

  • PAS reaction stains phagocytosed atypical mycobacteria in paraffin sections.

    abstract::In 4% formaldehyde-fixed, paraffin-embedded tissues, PAS negativity for mycobacteria in the literature has been axiomatic. However, recent observations of disseminated human mycobacteriosis have shown that intracellular (phagocytosed) organisms stained strongly positive with the routine PAS technique. This staining wa...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Pappolla MA,Mehta VT

    更新日期:1984-05-01 00:00:00

  • Accuracy of fine-needle aspiration of thyroid.

    abstract:CONTEXT:Fine-needle aspiration has become an accepted and cost-effective procedure for rapid diagnosis of thyroid lesions. The routine use of fine-needle aspiration has reduced the rate of unnecessary surgery for thyroid nodules. OBJECTIVES:To determine the accuracy of fine-needle aspiration biopsy diagnosis and to di...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/0003-9985(2001)125<0484:AOFNAO>2.0.CO;2

    authors: Amrikachi M,Ramzy I,Rubenfeld S,Wheeler TM

    更新日期:2001-04-01 00:00:00

  • Medullary carcinoma of the breast. A multicenter study of its diagnostic consistency.

    abstract::Nine pathologists from different institutions reviewed in a double-blind study 16 breast tumors previously indexed as typical medullary carcinoma, atypical medullary carcinoma, or infiltrative ductal carcinoma. A set of 16 slides was circulated two times among the nine pathologists. The diagnoses of typical and atypic...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,多中心研究

    doi:

    authors: Rigaud C,Theobald S,Noël P,Badreddine J,Barlier C,Delobelle A,Gentile A,Jacquemier J,Maisongrosse V,Peffault de Latour M

    更新日期:1993-10-01 00:00:00

  • College of American Pathologists Cancer Protocols: Optimizing Format for Accuracy and Efficiency.

    abstract:CONTEXT:-The data in College of American Pathologists cancer protocols have to be presented effectively to health care providers. There is no consensus on the format of those protocols, resulting in various designs among pathologists. Cancer protocols are independently created by site-specific experts, so there is inco...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.5858/arpa.2015-0237-OA

    authors: Strickland-Marmol LB,Muro-Cacho CA,Barnett SD,Banas MR,Foulis PR

    更新日期:2016-06-01 00:00:00

  • Mre11 expression in atypical adenomatous hyperplasia and adenocarcinoma of the lung.

    abstract:CONTEXT:The Mre11-Rad50-NBS1 complex plays an important role in telomere maintenance. Recently, it has been proposed that alterations in Mre11 function may be contributing factors in the development of some tumors. Moreover, mutations of Mre11 have been demonstrated to cause reduced Mre11 immunostaining. OBJECTIVE:To ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/1543-2165(2006)130[1330:MEIAAH]2.0.CO;2

    authors: Nakanishi K,Kumaki F,Hiroi S,Mukai M,Ikeda E,Kawai T

    更新日期:2006-09-01 00:00:00

  • Sputum examination in the diagnosis of Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome.

    abstract::To examine the role of sputum examination in the diagnosis of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome (AIDS), we conducted a prospective study of 30 patients with AIDS or suspected AIDS. Sputum was obtained just prior to bronchoscopy by spontaneous cough (20 patients) or ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: del Rio C,Guarner J,Honig EG,Slade BA

    更新日期:1988-12-01 00:00:00

  • Embolism of brain tissue to the lung in a neonate. Report of a case and review of the literature.

    abstract::Embolic cerebellar tissue was observed microscopically in the pulmonary and coronary arteries of a full-term male infant who died 1 hour after forceps delivery. Although embolization of brain tissue has been well documented after severe head trauma in adults and children, to our knowledge only 17 cases associated with...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,评审

    doi:

    authors: Hauck AJ,Bambara JF,Edwards WD

    更新日期:1990-02-01 00:00:00

  • Age and laboratory costs for hospitalized medical patients.

    abstract:OBJECTIVE:To examine the hypothesis that older hospitalized patients have higher laboratory costs than younger patients in the same severity-adjusted diagnosis-related group (DRG). DESIGN:We obtained hospital case mix data sets (1995-1997) from the Massachusetts Division of Health Care Finance and Policy. We selected ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:10.1043/0003-9985(2003)127<169:AALCFH>2.0.CO;2

    authors: Brimhall BB,Dean T,Hunt EL,Siegrist RB,Reiquam W

    更新日期:2003-02-01 00:00:00

  • Ultrastructure of the parotid duct. Cytochemical studies of the striated duct and papillary cystadenoma lymphomatosum of the human parotid gland.

    abstract::To our knowledge, there are currently no reports in the literature that discuss the fine structure of the striated ducts of the human parotid gland. Papillary cystadenoma lymphomatosum (Warthin's tumor) is a benigh neoplasm found almost exclusively in the parotid gland that represents about 16% of all neoplasms of the...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Cutler LS,Chaudhry A,Innes DJ Jr

    更新日期:1977-08-01 00:00:00

  • Reversibility of myocyte injury in moderate and severe acute rejection in cyclosporine-treated cardiac transplant patients.

    abstract::Myocyte injury in acute cardiac transplant rejection was studied ultrastructurally in 45 patients. Mild myocyte injury consisted of increased glycogen granules and mitochondria, and condensation and streaming of myofilaments. More severe cell injury was characterized by clumping of Z-band material at the periphery of ...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章

    doi:

    authors: Myles JL,Ratliff NB,McMahon JT,Golding LR,Hobbs RE,Rincon G,Sterba RW,Stewart R

    更新日期:1987-10-01 00:00:00

  • Quality and liability issues with the Papanicolaou smear: lessons from the science of error prevention.

    abstract::The pathologists and cytologists who study Papanicolaou smears perform a highly successful cancer screening test in a low-prevalence population. This leads to a mathematically inevitable false-negative error rate even in the most competent professional hands. The US judicial system supports a public expectation of per...

    journal_title:Archives of pathology & laboratory medicine

    pub_type: 杂志文章,评审

    doi:

    authors: Derman H

    更新日期:1997-03-01 00:00:00