Abstract:
:A rapid and sensitive assay was developed to detect CAA-->AAA mutations at codon 61 of Ha-ras. The region surrounding codon 61 was amplified by the polymerase chain reaction (PCR) using one primer containing a mismatch at the second position of codon 60. Using this primer creates an Msel restriction enzyme site if codon 61 carries the C.G-->A.T transversion. An aliquot of the second PCR primer was 5'-end-labeled with 32P to increase the sensitivity of detection of the PCR product. After cleavage with Msel, DNA was electrophoresed on a nondenaturing polyacrylamide gel, and the products were visualized by autoradiography. The sensitivity of this assay was such that the mutation could be detected when present in only one of 200 alleles. DNA samples from spontaneous Crl:CD-1(ICR)BR mouse liver tumors were analyzed using this method. Nine of 38 samples contained the mutation, and in one of those nine, the mutation had not been previously detected by either direct sequencing of tumor DNA or by sequencing the DNA from NIH 3T3 cells transfected with the tumor DNA.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Shinder GA,Manam S,Nichols WWdoi
10.1002/mc.2940070409subject
Has Abstractpub_date
1993-01-01 00:00:00pages
263-7issue
4eissn
0899-1987issn
1098-2744journal_volume
7pub_type
杂志文章abstract::The aims of the present study are to investigate sub-cellular location, differential expression in different cancer stages and functional role of FAM134B in colon cancer development. FAM134B expression was studied and quantified at protein and mRNA levels in cell lines using immunocytochemistry, Western blot and real-...
journal_title:Molecular carcinogenesis
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journal_title:Molecular carcinogenesis
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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journal_title:Molecular carcinogenesis
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pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1002/mc.20508
更新日期:2009-04-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20457
更新日期:2009-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20310
更新日期:2007-10-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22217
更新日期:2015-11-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:1999-09-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2018-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.22060
更新日期:2014-12-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00