Tuber borchii fruit body: 2-dimensional profile and protein identification.

Abstract:

:The formation of the fruit body represents the final phase of the ectomycorrhizal fungus T. borchii life cycle. Very little is known concerning the molecular and biochemical processes involved in the fructification phase. 2-DE maps of unripe and ripe ascocarps revealed different protein expression levels and the comparison of the electropherograms led to the identification of specific proteins for each developmental phase. Associating micropreparative 2-DE to microchemical approaches, such as N-terminal sequencing and 2-D gel-electrophoresis mass-spectrometry, proteins playing pivotal roles in truffle physiology were identified.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Pierleoni R,Buffalini M,Vallorani L,Guidi C,Zeppa S,Sacconi C,Pucci P,Amoresano A,Casbarra A,Stocchi V

doi

10.1016/j.phytochem.2004.02.012

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

813-20

issue

7

eissn

0031-9422

issn

1873-3700

pii

S003194220400072X

journal_volume

65

pub_type

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