Histopathological and immunohistochemical detection of protective effects of University of Wisconsin solution supplemented with iloprost on donor lung damage.


OBJECTIVE:Histopathological evaluation and immunohistochemical markers of surfactant B and CD34 were used to detect alveolar type II cell and pulmonary endothelial cell damage in order to assess the efficacy on donor lung protection of University of Wisconsin (UW) solution supplementation with iloprost. METHODS:Twelve rats were divided into two groups: UW solution was used alone in group I, and UW iloprost solution in group II. Lung samples were taken at regular intervals for pathological examination to evaluate alveolar cell integrity with hematoxylin and eosin staining. Preservation, of alveolar type II cell and pulmonary endothelial cells was assessed using surfactant B and CD34 immunomarkers, respectively. RESULTS:In both groups, alveolar integrity, surfactant, and CD34 revealed time-dependent, progressive damage, although this deterioration was less apparent among the iloprost-supplemented group. Alveolar integrity was better preserved at 4, 6, 8, 12, and 48 hours among group II rate. Surfactant staining showed significantly more deterioration at 12 and 24 hours in group I. Similarly, CD34 demonstrated significantly more injury at 6, 12, 24, and 48 hours in group I. CONCLUSION:Although progressive lung tissue damage assessed by histopathological and immunohistochemical methods was observed in both groups, our findings suggest less deterioration in the iloprost-supplemented group.


Transplant Proc


Ustundag N,Bozkurt AK,Demirkaya A,Koksal C,Mayda AS




Has Abstract


2004-06-01 00:00:00














  • Thromboelastography as a Predictor of Outcomes Following Liver Transplantation.

    abstract:BACKGROUND:Thromboelastography (TEG) has been used perioperatively during liver transplantation (LT) to provide a real-time global hemostasis assessment for targeted blood product replacement. We aimed to analyze the relationship between post-LT TEG results and outcomes. METHODS:We retrospectively analyzed patients un...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Trautman CL,Palmer WC,Taner CB,Canabal JM,Getz T,Goldman A,Heckman MG,Diehl NN,Lee DD,Stancampiano FF

    更新日期:2017-11-01 00:00:00

  • Artificial islets from hybrid spheroids of three pancreatic cell lines.

    abstract::Pancreatic islets have been the focus of recent studies exploring the pathologic mechanisms of diabetes mellitus as well as more effective and radical treatments for this disease. Islet transplantation is a promising therapeutic strategy; however, isolation of pancreatic islets for this purpose has been challenging, b...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Jo YH,Jang IJ,Nemeno JG,Lee S,Kim BY,Nam BM,Yang W,Lee KM,Kim H,Takebe T,Kim YS,Lee JI

    更新日期:2014-05-01 00:00:00

  • Is retrieval of bone material from multiorgan donors effective enough to cover demand for biostatic bone tissue grafts in Poland?

    abstract::In Poland there is growing demand for biostatic allogeneic bone transplantation mostly for traumatologic operations and orthopedic reconstructions. The bone material is primarily harvested during postmortem examinations in forensic and pathology laboratories. Nevertheless, the collected amounts are not sufficient, so ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Bohatyrewicz A,Bohatyrewicz R,Dobiecki K,Kotrych D,Kamiński A,Zienkiewicz M,Zietek P,Dziedzic-Gocławska A

    更新日期:2006-01-01 00:00:00

  • Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome.

    abstract:BACKGROUND:Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Anazawa T,Balamurugan AN,Papas KK,Avgoustiniatos ES,Ferrer J,Matsumoto S,Sutherland DE,Hering BJ

    更新日期:2010-07-01 00:00:00

  • Organ donation: acceptance and refusal among users of the public health system from Brazil.

    abstract::The present study sought to identify the perception of a given group of users of the Brazilian Public Health System (Sistema Unico de Saúde) regarding organ donation and to implement an educational policy. Structured interviews were conducted with the aim of describing the profile of donor and nondonor subjects, the i...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sasso-Mendes KD,Curvo PA,Silveira RC,Galvão CM

    更新日期:2008-04-01 00:00:00

  • De Novo Malignancy Following Adult-to-Adult Living Donor Liver Transplantation Focusing on Posttransplantation Lymphoproliferative Disorder.

    abstract:OBJECTIVE:In patients with living donor liver transplantation (LDLT), late-onset complications sometimes develop because of long-term use of immunosuppressive drugs. One of the immunosuppressive drug-related complications is de novo malignancies resulting in reduced survival. PATIENTS AND METHODS:Among 153 patients un...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Mizuno S,Hayasaki A,Ito T,Fujii T,Iizawa Y,Kato H,Murata Y,Tanemura A,Kuriyama N,Azumi Y,Kishiwada M,Usui M,Sakurai H,Isaji S

    更新日期:2018-11-01 00:00:00

  • Clinical utility of real-time polymerase chain reaction to quantify cytomegalovirus replication in allogeneic stem cell transplant recipients with different prevention strategies.

    abstract::Cytomegalovirus (CMV) end-organ disease is a serious, frequent complication after allogenic stem cell transplantation (Allo-SCT). There are two prevention strategies: universal prophylaxis and preemptive therapy. Preemptive therapy is administered based on the results of sensitive techniques that detect the viral infe...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: De la Cruz-Vicente F,Perez-Romero P,Aguilar-Guisado M,Cisneros-Herreros JM,Urbano-Ispizua A,Espigado I

    更新日期:2010-10-01 00:00:00

  • Asian Indian donor marrow registry: All India Institute of Medical Sciences experience.

    abstract::A major limitation in hematopoietic stem cell transplantation (HSCT) is the availability of a genetically matched donor, particularly with respect to the human leukocyte antigens (HLA)-linked immune response genes located on chromosome 6 in humans. During the last 5 years, a total of 688 patients requiring HSCT underw...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Kanga U,Panigrahi A,Kumar S,Mehra NK

    更新日期:2007-04-01 00:00:00

  • Liver Transplantation With Livers From Octogenarians and a Nonagenarian.

    abstract:INTRODUCTION:A shortage of deceased donors has compelled the use of extended-criteria donor organs in liver transplantation. The purpose of this study was to evaluate the impact of using deceased donors older than 80 years. MATERIALS AND METHODS:We retrospectively evaluated 13 patients who received a liver graft from ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Dirican A,Soyer V,Koc S,Yagci MA,Sarici B,Onur A,Unal B,Yilmaz S

    更新日期:2015-06-01 00:00:00

  • Continuous Paravertebral Infusions as an Effective Adjunct for Postoperative Pain Management in Living Liver Donors: A Retrospective Observational Study.

    abstract::We analyzed the effectiveness of paravertebral-block for immediate postoperative pain control in living liver donors. Specifically, we sought to determine whether or not the addition of paravertebral catheters with continuous ropivacaine infusion would decrease postoperative opioid use and reduce the incidence of adve...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Mistry K,Hutchins J,Leiting J,Mangalick K,Pruett T,Chinnakotla S

    更新日期:2017-03-01 00:00:00

  • Donor-reactive cytokine production after HLA-identical living related kidney transplantation: a protein-array analysis.

    abstract::In the present pilot study, we investigated which proteins are produced after donor stimulation of peripheral blood mononuclear cells from recipients of HLA-identical living related kidney transplant. We used a protein-array analysis to determine cytokines, chemokines, and growth factors in supernatant from donor-stim...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Gerrits JH,van de Wetering J,Weimar W,van Besouw NM

    更新日期:2006-11-01 00:00:00

  • Experience with single-dose daclizumab in the prevention of acute rejection in heart transplantation.

    abstract:INTRODUCTION:Daclizumab is a monoclonal antibody that binds to the interleukin-2 receptor. It has been used as induction therapy in heart transplantation with two to five repeated administrations over several weeks. The objective of our study was to estimate the efficacy and safety of induction therapy with only one do...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Cuppoletti A,Perez-Villa F,Vallejos I,Roig E

    更新日期:2005-11-01 00:00:00

  • Urological complications in kidney transplantation: ureterocystostomy versus uretero-ureterostomy.

    abstract::In our initial experience of kidney transplantation, we performed an extravesical uretero-cystostomy (U-C), but in 1997 we shifted to a uretero-ureterostomy (U-U) with the aim of reducing early and late urological complications. A data base was constructed to compare the incidence, donor and recipient risk factors, tr...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Faenza A,Nardo B,Fuga G,Liviano-D'Arcangelo G,Grammatico F,Montalti R,Bertelli R,Beltempo P,Puviani L

    更新日期:2005-07-01 00:00:00

  • Treatment of acute graft-vs-host disease after simultaneous pancreas-kidney transplantation: a case report.

    abstract::Whereas neutropenia is common after solid-organ transplantation, graft-vs-host disease is unusual, especially after simultaneous pancreas-kidney transplantation. Most cases reported in the literature give few details of treatment approach, and all were fatal. A 45-year-old man with diabetes underwent simultaneous panc...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Osband AJ,Laskow DA,Mann RA

    更新日期:2010-11-01 00:00:00

  • Expression of HLA molecules on peripheral blood lymphocytes: a useful monitoring parameter in cardiac transplantation.

    abstract::During the rejection process of cardiac allografts, the expression of HLA antigens increases on various graft tissues, ie, the myocardium and the interstitial structures. However, in this type of transplant there is a paucity of knowledge about HLA expression on recipient cells, such as peripheral blood mononuclear ce...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Blanco-Garcia RM,López-Alvarez MR,Pascual-Figal DA,Polo-Garcia L,Guerra N,Campillo JA,Muro M,Garcia-Alonso AM,Marin-Moreno I,Valdes M,Alvarez-López MR,Minguela A

    更新日期:2007-09-01 00:00:00

  • Successful Treatment of Recurrent Focal Segmental Glomerulosclerosis After Transplantation in Children: A Single-Center Experience.

    abstract:OBJECTIVE:We aim to report our experience managing cases of recurrent focal segmental glomerulosclerosis (FSGS) in a group of pediatric renal transplant recipients. METHODS:This study was a retrospective chart review of pediatric patients who had their first kidney transplant at King Faisal Specialist Hospital & Resea...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Alhasan KA,Alherbish A,Osman A,Kari JA,Almojalli H

    更新日期:2019-03-01 00:00:00

  • Synergistic effect of cold and warm ischemia time on postoperative graft function and outcome in human liver transplantation.

    abstract::Prolonged cold ischemia time (CIT) during graft preservation and warm ischemia time (WIT) during rewarming time have been reported to cause postoperative graft dysfunction after orthotopic liver transplantation (OLT). However, the effects of both CIT and WIT in combination on patient and graft survivals are not yet de...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Totsuka E,Fung JJ,Hakamada K,Ohashi M,Takahashi K,Nakai M,Morohashi S,Morohashi H,Kimura N,Nishimura A,Ishizawa Y,Ono H,Narumi S,Sasaki M

    更新日期:2004-09-01 00:00:00

  • Holmium Intraductal Laser Lithotripsy of Biliary Stones in Liver Grafts.

    abstract:BACKGROUND:Biliary stones after liver transplantation (LT) rarely occur but a focus on those complications and their treatment is needed. PATIENTS AND METHODS:In total, 390 adult patients who underwent an LT from July 2004 to July 2014 entered the study. Biliary complications and notably biliary stones after LT were i...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Maggi U,Paone G,Lauro R,Fornoni G,Chierici A,Nencioni M,Caruso A,Rossi G

    更新日期:2016-03-01 00:00:00

  • Listing Malignant Melanoma Patients for Renal Transplantation.

    abstract:BACKGROUND:Melanoma is an immune responsive malignancy and the need for immunosuppression for successful transplantation may lead to recurrent disease. The recommended waiting time is unknown with various groups recommending anywhere from no wait to 5 years. METHODS:In this single-center, retrospective observational s...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Qaqish S,Datta N,Bunnapradist S,Lum EL

    更新日期:2020-12-01 00:00:00

  • Perioperative considerations in HIV-infected liver transplanted patients.

    abstract:UNLABELLED:The introduction of highly active antiretroviral therapy (HAART) has improved survival in HIV patients, allowing them to undergo liver transplantation (OLT) in cases of end-stage liver disease. HIV patients show a higher incidence of pulmonary hypertension. The aim of this study was to evaluate pulmonary and...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Costa MG,Pompei L,Chiarandini P,Lugano M,Baccarani U,Tavio M,Della Rocca G

    更新日期:2009-05-01 00:00:00

  • Recurrent Hepatitis B and D Virus Infection in a Liver Transplant Recipient.

    abstract::Hepatitis B virus (HBV) and hepatitis delta virus (HDV) co-infections progress rapidly and lead to cirrhosis. In Japan, the prevalence of HBV and HDV co-infected patients is low. Therefore, there are few reports of patients with HBV and HDV co-infection having undergone liver transplantation. Herein, we report a rare ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Miyaaki H,Tamada Y,Hayashi K,Taura N,Miuma S,Shibata H,Soyama A,Hidaka M,Takatsuki M,Eguchi S,Nakao K

    更新日期:2017-01-01 00:00:00

  • Gender-dependent histone deacetylases injury may contribute to differences in liver recovery rates of male and female mice.

    abstract:BACKGROUND:Hepatocytes undergo DNA synthesis shortly after liver damage triggered by partial hepatectomy (PH). This study revealed that the rate of liver damage recovery is gender-dependent. Furthermore, histone deacetylase (HDAC) epigenetic factors were discovered, at least in part, to govern the different liver recov...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Wang Y,Ye F,Ke Q,Wu Q,Yang R,Bu H

    更新日期:2013-03-01 00:00:00

  • Nutritional outcomes following pediatric intestinal transplantation.

    abstract:BACKGROUND:This study sought to describe the long-term nutritional outcomes of children after intestinal transplant (SBT). METHODS:Between 1991 and March 2005, 30 children received 33 SBT at a single center. Eligibility criteria included patient and graft survival >6 months. Weight, height, albumin, prealbumin, zinc (...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Venick RS,Farmer DG,Saikali D,Gordon S,Colangelo J,Reyen L,McDiarmid SV,Vargas JH,Ament ME,Busuttil RW

    更新日期:2006-07-01 00:00:00

  • Reproducible model for kidney autotransplantation in pigs.

    abstract:OBJECTIVE:Several porcine models have been employed to study mechanisms of warm ischemia, cold ischemia, and ischemia reperfusion injury, but the technical/surgical aspects of these models and their possible pitfalls have not been fully described in detail. The goal of the present study was to develop and optimize a po...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Jochmans I,Lerut E,Heedfeld V,Wylin T,Pirenne J,Monbaliu D

    更新日期:2009-10-01 00:00:00

  • Low-dose dopamine in kidney transplantation.

    abstract:BACKGROUND:The use of low-dose dopamine (LDD; 0.5-2.5 mug/kg/min) in kidney transplant recipients seeks to increase urine output, prevent arterial vasospasm, and reduce the incidence of acute tubular necrosis. The aim of this study was to evaluate the effect of LDD in the early postoperative period (12 hours) among kid...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Ciapetti M,di Valvasone S,di Filippo A,Cecchi A,Bonizzoli M,Peris A

    更新日期:2009-12-01 00:00:00

  • Predicting Short- and Long-Term Outcomes in Adult Heart Transplantation: Clinical Utility of MELD-XI Score.

    abstract:BACKGROUND:Model for End-Stage Liver Disease scoring system excluding international normalized ratio (MELD-XI) has been related with worse outcomes after heart transplantation (HT). However, according to standards in prognostic models research, before implementing a risk score for daily clinical decision-making, its pe...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Ortiz-Bautista C,García-Cosio MD,Lora-Pablos D,Ponz-de Antonio I,Rodríguez-Chaverri A,Morán-Fernández L,de Juan-Bagudá J,Pérez-de la Sota E,Cortina-Romero JM,Arribas-Ynsaurriaga F,Delgado-Jiménez JF

    更新日期:2018-12-01 00:00:00

  • The rate of organ and tissue donation after brain death: causes of donation failure in a Romanian university city.

    abstract:AIM:To evaluate the rate of organ donation after brain death in 2 tertiary care medical centers of a Romanian city, the reasons for donation exclusion (donors or organs), and identification of potential strategies for improvement. MATERIAL AND METHODS:The study retrospectively evaluated potential organ donors with bra...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Grigoraş I,Blaj M,Florin G,Chelarescu O,Craus C,Neagu R

    更新日期:2010-01-01 00:00:00

  • A logistic regression model for predicting health-related quality of life in kidney transplant recipients.

    abstract:BACKGROUND:To develop a logistic regression model capable of predicting health-related quality of life (HRQOL) among kidney transplant recipients and determine its accuracy. METHODS:Three groups of patients were selected: 70 healthy controls, 136 kidney transplant patients as a derivation set, and another 110 kidney t...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Khedmat H,Karami GR,Pourfarziani V,Assari S,Rezailashkajani M,Naghizadeh MM

    更新日期:2007-05-01 00:00:00

  • Efficacy of the cardiac rehabilitation program in patients with end-stage heart failure, heart transplant patients, and left ventricular assist device recipients.

    abstract:OBJECTIVE:The objective of this study was to assess the effects of cardiac rehabilitation on the functional capacity, pulmonary functions, quality of life, and psychological state of patients who had heart failure (HF), heart transplantation (HTx), or a left ventricular assist device (LVAD). METHODS:An 8-week exercise...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Karapolat H,Engin C,Eroglu M,Yagdi T,Zoghi M,Nalbantgil S,Durmaz B,Kirazlı Y,Ozbaran M

    更新日期:2013-11-01 00:00:00

  • Use of Prograf (FK 506) as rescue therapy for refractory rejection after liver transplantation. US Multicenter FK 506 Liver Study Group.

    abstract::This report describes the clinical characteristics and demographics of patients enrolled into this rescue trial for patients experiencing refractory rejection after liver transplantation. Actuarial graft and patient survival at 12 months postconversion was 50% and 72%, respectively. Actual treatment success at 3 month...

    journal_title:Transplantation proceedings

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Klintmalm GB,Goldstein R,Gonwa T,Wiesner RH,Krom RA,Shaw BW Jr,Stratta R,Ascher NL,Roberts JW,Lake J

    更新日期:1993-02-01 00:00:00