Molecular analysis of the Neurospora clock: cloning and characterization of the frequency and period-4 genes.

Abstract:

:Genetic analysis of Neurospora crassa has identified many mutants that affect the biological clock. In this article we review the cloning of two of these genes, frq and prd-4. Both genes were isolated using a chromosome walk technique. Subcloning experiments and subsequent Northern analysis of frq implicate the importance of two transcripts that emanate from this locus. In preliminary data, no protein-coding region is evident in the smaller transcript; the larger transcript contains a 962-amino acid open reading frame. The open reading frame shows limited homology to per, a clock gene identified in Drosophila. Sequence analysis of all existing frq alleles suggests that the defect in each case lies within the open reading frame. Successful cloning of the prd-4 gene required walking a distance of greater than 40 kb. A physical map of this region has been constructed using restriction analysis. The dominance-recessive relationship of prd-4 and prd-4+ was established by examining the period lengths of strains harboring a wide range of prd-4/prd-4+ nuclear ratios.

journal_name

Chronobiol Int

authors

Aronson BD,Johnson KA,Liu Q,Dunlap JC

doi

10.3109/07420529209064532

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

231-9

issue

3

eissn

0742-0528

issn

1525-6073

journal_volume

9

pub_type

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