Abstract:
:To investigate the dynamic control of cardiac ATP synthesis, we simultaneously determined the time course of mitochondrial oxygen consumption with the time course of changes in high-energy phosphates following steps in cardiac energy demand. Isolated isovolumically contracting rabbit hearts were perfused with Tyrode's solution at 28 degrees C (n = 7) or at 37 degrees C (n = 7). Coronary arterial and venous oxygen tensions were monitored with fast-responding oxygen electrodes. A cyclic pacing protocol in which we applied 64 step changes between two different heart rates was used. This enabled nuclear magnetic resonance measurement of the phosphate metabolites with a time resolution of approximately 2 seconds. Oxygen consumption changed after heart-rate steps with time constants of 14 +/- 1 (mean +/- SEM) seconds at 28 degrees C and 11 +/- 1 seconds at 37 degrees C, which are already corrected for diffusion and vascular transport delays. Doubling of the heart rate resulted in a significant decrease in phosphocreatine (PCr) content (11% at 28 degrees C, 8% at 37 degrees C), which was matched by an increase in inorganic phosphate (P(i)) content, although oxygen supply was shown to be nonlimiting. The time constants for the change of both P(i) and PCr content, approximately 5 seconds at 28 degrees C and 2.5 seconds at 37 degrees C, are significantly smaller than the respective time constants for oxygen consumption.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Circ Resjournal_title
Circulation researchauthors
Eijgelshoven MH,van Beek JH,Mottet I,Nederhoff MG,van Echteld CJ,Westerhof Ndoi
10.1161/01.res.75.4.751subject
Has Abstractpub_date
1994-10-01 00:00:00pages
751-9issue
4eissn
0009-7330issn
1524-4571journal_volume
75pub_type
杂志文章abstract::As described in earlier reviews in this series on the molecular basis of hypertrophic cardiomyopathy (HCM), HCM is one of the archetypal monogenic cardiovascular disorders to be understood at the molecular level. Twenty years after the discovery of the first HCM disease gene, genetic studies still confirm that HCM is ...
journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2014-06-06 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.118.312821
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doi:10.1161/01.res.84.3.266
更新日期:1999-02-19 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.45.5.677
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pub_type: 杂志文章
doi:10.1161/01.res.41.2.172
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pub_type: 杂志文章
doi:10.1161/01.RES.0000150594.95988.45
更新日期:2004-12-10 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.308544
更新日期:2016-04-15 00:00:00
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更新日期:1987-07-01 00:00:00
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更新日期:2007-08-17 00:00:00
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更新日期:1996-01-01 00:00:00
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更新日期:1998-01-09 00:00:00
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更新日期:1988-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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更新日期:1998-11-02 00:00:00
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更新日期:1978-08-01 00:00:00