当前位置： SCI文献检索 > GLIA期刊下所有文献 > Blood monocytes and spleen macrophages differentiate into microglia-like cells on monolayers of astrocytes: membrane currents.

Blood monocytes and spleen macrophages differentiate into microglia-like cells on monolayers of astrocytes: membrane currents.

Abstract：

:Microglia, the resident macrophages of the central nervous system (CNS), can be distinguished from most other cells of the myelomonocytic lineage by a distinct pattern of membrane currents. In the accompanying paper we have shown that the characteristic morphological feature of microglia, ramification, develops both in microglia and other myelomonocytic cells when they are cocultured with astrocytes. We therefore propose that the electrophysiological properties of microglia also develop under the influence of astrocytes, and, moreover, that these properties can also be induced in other cells of the myelomonocytic lineage. Microglia cultured on poly-d-lysine or on a monolayer of fibroblasts possess an inwardly rectifying K(+)-current only, which is of composite nature. In single-channel recordings two types of K(+)-channels are found: i) a noninactivating channel with a conductance of 43pS, and ii) an inactivating channel with 32pS. Microglia cultured on a monolayer of astrocytes additionally develop an outward K(+)-current and a Na(+)-current. The electric parameters of activation and inactivation of the microglial Na(+)-current are identical to those of the neuronal Na(+)-current. Monocytes from peripheral blood and macrophages from spleen exhibit no inward currents. However, when these cells are cocultured with astrocytes they develop microglia-like membrane currents, including the inward and outward K(+)-rectifyer and the Na(+)-current. By contrast, on fibroblasts they retain their macrophage current profile. The expression of the microglia-like membrane currents in the mononuclear phagocytes is induced by a diffusible factor released from the astrocytes into the culture medium, since monocytes and microglia develop the mature microglial current profile, when cultured in astrocyte conditioned medium.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Glia

journal_title

Glia

authors

Schmidtmayer J,Jacobsen C,Miksch G,Sievers J

doi

10.1002/glia.440120403

subject

Has Abstract

pub_date

1994-12-01 00:00:00

pages

259-67

issue

eissn

0894-1491

issn

1098-1136

journal_volume

pub_type

杂志文章

在线工具

Blood monocytes and spleen macrophages differentiate into microglia-like cells on monolayers of astrocytes: membrane currents.