A plant mitochondrial sequence transcribed in transgenic tobacco chloroplasts is not edited.

abstract：:Retroviruses have evolved complex transcriptional enhancers and promoters that allow their replication in a wide range of tissue and cell types. Embryonic stem (ES) cells, however, characteristically suppress transcription of proviruses formed after infection by exogenous retroviruses and also of most members of the v...

journal_title：Molecular and cellular biology

authors： Schlesinger S,Goff SP

更新日期：2015-03-01 00:00:00

abstract：:The gene coding for the most abundant antifreeze protein (AFP) in the winter flounder was placed downstream of the Drosophila melanogaster hsp70 promoter and introduced into the D. melanogaster germ line by P-element-mediated transformation. In each of six transgenic strains tested, heat shock treatment induced the ex...

journal_title：Molecular and cellular biology

authors： Rancourt DE,Walker VK,Davies PL

更新日期：1987-06-01 00:00:00

abstract：:Special AT-rich sequence-binding protein 1 (SATB1) is a tissue-restricted genome organizer that provides a key link between DNA loop organization, chromatin modification/remodeling, and transcription factor association at matrix attachment regions (MARs). The SUMO E3 ligase PIAS1 enhances SUMO conjugation to SATB1 lys...

journal_title：Molecular and cellular biology

authors： Tan JA,Song J,Chen Y,Durrin LK

更新日期：2010-06-01 00:00:00

abstract：:The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at th...

journal_title：Molecular and cellular biology

authors： Hsu JM,Huang J,Meluh PB,Laurent BC

更新日期：2003-05-01 00:00:00

abstract：:Considering the link between plasma high-density lipoprotein (HDL) cholesterol levels and a protective effect against coronary artery disease as well as the suggested beneficial effects of retinoids on the production of the major HDL apolipoprotein (apo), apo A-I, the goal of this study was to analyze the influence of...

journal_title：Molecular and cellular biology

authors： Vu-Dac N,Schoonjans K,Kosykh V,Dallongeville J,Heyman RA,Staels B,Auwerx J

更新日期：1996-07-01 00:00:00

abstract：:Multiple isoforms of the protein tyrosine phosphatase CD45 are expressed on the surface of human T cells. Interestingly, the expression of these isoforms has been shown to vary significantly upon T-cell activation. In this report, we describe a novel cell line-based model system in which we can mimic the activation-in...

journal_title：Molecular and cellular biology

authors： Lynch KW,Weiss A

更新日期：2000-01-01 00:00:00

abstract：:Elevation of cellular cyclic AMP (cAMP) levels inhibits cell cycle reentry in a variety of cell types. While cAMP can prevent the activation of Raf-1 and extracellular signal-regulated kinases 1 and 2 (ERK1/2) by growth factors, we now show that activation of ERK1/2 by DeltaRaf-1:ER is insensitive to cAMP. Despite thi...

journal_title：Molecular and cellular biology

authors： Balmanno K,Millar T,McMahon M,Cook SJ

更新日期：2003-12-01 00:00:00

abstract：:Previous studies have shown that exposure of cells to high levels of replicational stress leads to permanent proliferation arrest that does not require p53. We have examined cellular responses to therapeutically relevant low levels of replicational stress that allow limited proliferation. Chronic exposure to low conce...

journal_title：Molecular and cellular biology

authors： Marusyk A,Wheeler LJ,Mathews CK,DeGregori J

更新日期：2007-08-01 00:00:00

abstract：:In mammals, the gene coding for apolipoprotein AI (apoAI), a protein of the plasma lipid transport system, is expressed only in the liver and the intestine. A series of plasmids containing various lengths of sequences flanking the 5' end of the human apoAI gene were constructed and assayed for transient expression aft...

journal_title：Molecular and cellular biology

authors： Sastry KN,Seedorf U,Karathanasis SK

更新日期：1988-02-01 00:00:00

abstract：:By using a retrovirus-derived vector system, we generated derivatives of the human colon cancer cell line HT29 that stably overexpress a full-length cDNA encoding the beta 1 isoform of rat protein kinase C (PKC). Two of these cell lines, PKC6 and PKC7, displayed an 11- to 15-fold increase in PKC activity when compared...

journal_title：Molecular and cellular biology

authors： Choi PM,Tchou-Wong KM,Weinstein IB

更新日期：1990-09-01 00:00:00

abstract：:The laminin alpha4 chain, a component of laminin-8 and -9, is expressed in basement membranes, such as those beneath endothelia, the perineurium of peripheral nerves, and around developing muscle fibers. Laminin alpha4-null mice presented with hemorrhages during the embryonic and neonatal period and had extensive blee...

journal_title：Molecular and cellular biology

authors： Thyboll J,Kortesmaa J,Cao R,Soininen R,Wang L,Iivanainen A,Sorokin L,Risling M,Cao Y,Tryggvason K

更新日期：2002-02-01 00:00:00

abstract：:Binding sites for six distinct nuclear factors on the 75-base-pair repeat of the Moloney murine leukemia virus enhancer have been identified by an electrophoretic mobility shift assay combined with methylation interference. Three of these factors, found in WEHI 231 nuclear extracts, which we have named LVa, LVb, and L...

journal_title：Molecular and cellular biology

authors： Speck NA,Baltimore D

更新日期：1987-03-01 00:00:00

abstract：:Ribosomal protein L5 binds specifically to 5S rRNA to form a complex that is a precursor to 60S subunit assembly in vivo. Analyses in yeast cells, mammalian cells, and Xenopus embryos have shown that the accumulation of L5 is not coordinated with the expression of other ribosomal proteins. In this study, the primary s...

journal_title：Molecular and cellular biology

authors： Wormington WM

更新日期：1989-12-01 00:00:00

abstract：:Protein acetylation has emerged as a means of controlling levels of mRNA synthesis in eukaryotic cells. Here we report that acetyl coenzyme A (acetyl-CoA) stimulates RNA polymerase II transcription in vitro in the absence of histones. The effect of acetyl-CoA on basal and activated transcription was studied in a human...

journal_title：Molecular and cellular biology

authors： Galasinski SK,Lively TN,Grebe De Barron A,Goodrich JA

更新日期：2000-03-01 00:00:00

abstract：:The levels of intramolecular plasmid recombination, following transfection of a plasmid substrate for homologous recombination into normal and immortally transformed cells, have been examined by two independent assays. In the first assay, recovered plasmid was tested for DNA rearrangements which regenerate a functiona...

journal_title：Molecular and cellular biology

authors： Finn GK,Kurz BW,Cheng RZ,Shmookler Reis RJ

更新日期：1989-09-01 00:00:00

abstract：:In order to identify catalytically important amino acid changes within the second-largest subunit of yeast RNA polymerase III, we mutagenized selected regions of its gene (RET1) and devised in vivo assays for both increased and decreased transcription termination by this enzyme. Using as the reporter gene a mutant SUP...

journal_title：Molecular and cellular biology

authors： Shaaban SA,Krupp BM,Hall BD

更新日期：1995-03-01 00:00:00

abstract：:The short form (S1b) of the prolactin receptor (PRLR) silences prolactin-induced activation of gene transcription by the PRLR long form (LF). The functional and structural contributions of two intramolecular disulfide (S-S) bonds within the extracellular subdomain 1 (D1) of S1b to its inhibitory function on the LF wer...

journal_title：Molecular and cellular biology

authors： Xie YL,Hassan SA,Qazi AM,Tsai-Morris CH,Dufau ML

更新日期：2009-05-01 00:00:00

abstract：:We have previously shown that activation of extracellular signal-regulated kinase (Erk) by epidermal growth factor (EGF) treatment was significantly decreased in mouse fibroblast cells expressing a mutant Shp-2 molecule lacking 65 amino acids in the SH2-N domain, Shp-2(Delta46-110). To address the molecular mechanism ...

journal_title：Molecular and cellular biology

authors： Shi ZQ,Yu DH,Park M,Marshall M,Feng GS

更新日期：2000-03-01 00:00:00

abstract：:Mutations in mitochondrial DNA (mtDNA) cause a variety of relatively rare human diseases and may contribute to the pathogenesis of other, more common degenerative diseases. This stimulates interest in the capacity of mitochondria to repair damage to mtDNA. Several recent studies have shown that some types of damage to...

journal_title：Molecular and cellular biology

authors： Pinz KG,Bogenhagen DF

更新日期：1998-03-01 00:00:00

abstract：:At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lin...

journal_title：Molecular and cellular biology

authors： Laker C,Kluge N,Stocking C,Just U,Franz MJ,Ostertag W,DeLamarter JF,Dexter M,Spooncer E

更新日期：1989-12-01 00:00:00

abstract：:Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two heat-sensitive and two cold-sensitive isolates were characterized. The growth of the four mutants is inhibited at their ...

journal_title：Molecular and cellular biology

authors： Moritz M,Pulaski BA,Woolford JL Jr

更新日期：1991-11-01 00:00:00

abstract：:In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesize...

journal_title：Molecular and cellular biology

authors： Morange M,Diu A,Bensaude O,Babinet C

更新日期：1984-04-01 00:00:00

abstract：:Two members of the heat shock transcription factor (HSF) family, HSF1 and HSF2, both function as transcriptional activators of heat shock gene expression. However, the inducible DNA-binding activities of these two factors are regulated by distinct pathways. HSF1 is activated by heat shock and other forms of stress, wh...

journal_title：Molecular and cellular biology

authors： Sistonen L,Sarge KD,Morimoto RI

更新日期：1994-03-01 00:00:00

abstract：:To investigate the role of insulin receptor substrate 1 (IRS-1) and IRS-2, the two ubiquitously expressed IRS proteins, in adipocyte differentiation, we established embryonic fibroblast cells with four different genotypes, i.e., wild-type, IRS-1 deficient (IRS-1(-/-)), IRS-2 deficient (IRS-2(-/-)), and IRS-1 IRS-2 dou...

journal_title：Molecular and cellular biology

authors： Miki H,Yamauchi T,Suzuki R,Komeda K,Tsuchida A,Kubota N,Terauchi Y,Kamon J,Kaburagi Y,Matsui J,Akanuma Y,Nagai R,Kimura S,Tobe K,Kadowaki T

更新日期：2001-04-01 00:00:00

abstract：:We have previously reported that antibodies to phosphotyrosine recognize the phosphorylated forms of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) receptors (Zippel et al., Biochim. Biophys. Acta 881:54-61, 1986, and Sturani et al., Biochem. Biophys. Res. Commun. 137:343-350, 1986). In this r...

journal_title：Molecular and cellular biology

authors： Sturani E,Zippel R,Toschi L,Morello L,Comoglio PM,Alberghina L

更新日期：1988-03-01 00:00:00

abstract：:Downregulation of specific transcripts is one of the mechanisms utilized by eukaryotic checkpoint systems to prevent cell cycle progression. Here we identified and explored such a mechanism in the yeast Saccharomyces cerevisiae. It involves the Mec1-Rad53 kinase cascade, which attenuates G(2)/M-specific gene transcrip...

journal_title：Molecular and cellular biology

authors： Yelamanchi SK,Veis J,Anrather D,Klug H,Ammerer G

更新日期：2014-02-01 00:00:00

abstract：:Histone N-terminal tails are central to the processes that modulate nucleosome structure and function. We have studied the contribution of core histone tails to the structure of a single nucleosome and to a histone (H3-H4)(2) tetrameric particle assembled on a topologically constrained DNA minicircle. The effect of hi...

journal_title：Molecular and cellular biology

authors： Morales V,Richard-Foy H

更新日期：2000-10-01 00:00:00

abstract：:Thyroid hormone (T(3)) activates nuclear receptor transcription factors, encoded by the TRalpha (NR1A1) and TRbeta (NR1A2) genes, to regulate target gene expression. Several TR isoforms exist, and studies of null mice have identified some unique functions for individual TR variants, although considerable redundancy oc...

journal_title：Molecular and cellular biology

authors： Williams GR

更新日期：2000-11-01 00:00:00

abstract：:Histone H1 is an abundant and essential component of chromatin whose precise role in regulating gene expression is poorly understood. Here, we report that a major target of H1-mediated regulation in embryonic stem (ES) cells is the X-linked Rhox homeobox gene cluster. To address the underlying mechanism, we examined t...

journal_title：Molecular and cellular biology

authors： Maclean JA,Bettegowda A,Kim BJ,Lou CH,Yang SM,Bhardwaj A,Shanker S,Hu Z,Fan Y,Eckardt S,McLaughlin KJ,Skoultchi AI,Wilkinson MF

更新日期：2011-03-01 00:00:00

abstract：:Hepatocyte nuclear factor 4 (HNF-4), a highly conserved member of the steroid hormone receptor superfamily critical for development and liver-specific gene expression, is very similar to another superfamily member, retinoid X receptor alpha (RXR alpha), in overall amino acid sequence and DNA binding specificity. Since...

journal_title：Molecular and cellular biology

authors： Jiang G,Nepomuceno L,Hopkins K,Sladek FM

更新日期：1995-09-01 00:00:00