Genetic diversity among Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis strains using repetitive element polymorphism-PCR.

Abstract:

:Repetitive element polymorphism-PCR (REP-PCR) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. Using the MB1 primer, REP-PCR fingerprints from 110 Bacillus strains within the "B. cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. All Bacillus anthracis strains tested were found to be monomorphic by fluorophore-enhanced REP-PCR (FERP) fingerprinting using the MB1 primer. In contrast, other non- B. anthracis isolates displayed a high degree of polymorphism. Dendrogramic analysis revealed that the non- B. anthracis strains possessing the Ba813 chromosomal marker were divided into two clusters. One of the clusters shared identity with the B. cereus strains examined.

journal_name

Pol J Microbiol

authors

Brumlik MJ,Bielawska-Drózd A,Zakowska D,Liang X,Spalletta RA,Patra G,Delvecchio VG

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

215-25

issue

4

eissn

1733-1331

issn

2544-4646

journal_volume

53

pub_type

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