Abstract：
:The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology techniques. The aim of this study was to determine the effectiveness and usefulness of a real-time PCR method, when compared to the recommended bacteria culture method, in diagnostics of pathogens in water samples. The research concerned the detection and identification of main sanitary indicators of water such as: Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens. The analyses were conducted in water samples contaminated with the reference material (the aforementioned bacteria) and real environmental samples, which were examined for the presence of nucleic acid of: Salmonella spp., E. coli, S. aureus and C. perfringens using a real-time PCR method.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Rozwadowska B,Albertyńska M,Hudzik G,Okła H,Jasik KP,Słodki Jsubject
Has Abstractpub_date
2013-01-01 00:00:00pages
439-43issue
4eissn
1733-1331issn
2544-4646journal_volume
62pub_type
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