Abstract:
:Bloodstream infections (BSIs) are associated with a significantly increased risk of fatality. No report has been found about the molecular epidemiology of Enterobacteriaceae causing BSI in neonates in Poland. The aim of this work was to determine the antibiotic resistance profiles, virulence gene prevalence, the epidemiological and genetic relationships among the isolates from Enterobacteriaceae causing BSI in neonates with birth weight < 1501 g. Antimicrobial susceptibility testing was performed. PCR was performed to identify the presence of common beta-lactamase genes, virulence genes. PFGE and MLST were performed. The surveillance group contained 1,695 newborns. The incidence rate for BSIs was 5.9%, the fatality rate 15%. The most common species were Escherichia coli (n = 24) and Klebsiella pneumoniae (n = 16). CTX-M-15 was found in 6 E. coli, 8 K. pneumoniae, 1 Enterobacter cloacae strains. Among E. coli fimH (83.3%), ibeA (37.5%), neuC (20.8%) were the most frequent. PFGE demonstrated unique pulsotypes among E. coli. E. coli ST131 clone was found in 7 E. coli strains. PFGE of 16 K. pneumoniae strains showed 8 pulsotypes. Five isolates from one NICU belonged to one clone. MLST typing revealed 7 different ST with ST336 as the most prevalent. This study provides information about resistance, virulence and typing of Enterobacteriaceae strains causing BSI among neonates. E. coli and Klebsiella spp. isolated in this study have completely different epidemiology from each other.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Chmielarczyk A,Pobiega M,Wójkowska-Mach J,Romaniszyn D,Heczko PB,Bulanda Msubject
Has Abstractpub_date
2015-01-01 00:00:00pages
217-25issue
3eissn
1733-1331issn
2544-4646journal_volume
64pub_type
杂志文章abstract::Biologic prosthetics are increasingly used for the repair of abdominal wall hernia defects but can become infected as a result of peri- or early post-operative bacterial contamination. Data evaluating biofilm formation on biologic prosthetics is lacking. The aim of this study was to investigate the influence of differ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouse gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of rum...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::In this study we asked a question whether H. pylori LPS with or without LewisXY (Le) determinants as well as LBP (lipopolysaccharide binding protein) and sCD 14 molecules recognizing bacterial LPS may be involved in atherogenesis. Sera from 57 patients with coronary heart disease (CHD), 27 H. pylori infected dyspeptic...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Clinical diagnosis of hepatitis E viral (HEV) infection mainly relies on serological assays, and the current status of misdiagnoses regarding HEV infection is uncertain. In this study, patients with acute HEV infection were tested for anti-HEV IgM and IgG, a HEV antigen (Ag), and viral loads (HEV RNA). Serology was pe...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2020-025
更新日期:2020-09-01 00:00:00
abstract::In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification ofa genus-specific sequence in the Streptomyces' 16S rDNA gene. DNA band of the expected size (438 bp) was seen with al...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The aim of the study was to determine the influence of the presence or the absence of antibiotic input on the emergence and maintenance of resistance in commensal bacteria from food producing animals. The research material constituted E. coli isolates from two animal species: swine at different age from one convention...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::A total 44 isolates of S. enterica serovar Enteritidis (S. Enteritidis) belonged to three different phage types (PTs) 9a, 13a, 25 were analyzed by the technique of pulsed-field gel electrophoresis (PFGE). Thirty and three strains were from two outbreaks (central and southern regions of the Slovak Republic PTs 13a, 25)...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Unravelling of the interplay between the immune system and non-diphtheria corynebacteria would contribute to understanding their increasing role as medically important microorganisms. We aimed at the analysis of pro- (TNF, IL-1β, IL-6, IL-8, and IL-12p70) and anti-inflammatory (IL-10) cytokines produced by Jurkat T ce...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2019-045
更新日期:2019-12-01 00:00:00
abstract::Due to the rising use of antibiotics and as a consequence of their concentration in the environment an increasing number of antibiotic resistant bacteria is observed. The phenomenon has a hazardous impact on human and animal life. Sulfamethoxazole is one of the sulfonamides commonly detected in surface waters and soil...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::The aim of this study was to determine if there are quantitative differences in Candida fungi between pediatric patients with Crohn's disease (before and after exclusive enteral nutrition (EEN), and the biologic therapy with anti-tumor necrosis factor alpha - (IFX)), and healthy controls. DNA was isolated from fecal s...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.21307/pjm-2019-014
更新日期:2019-01-01 00:00:00
abstract::Two hundred and fifty bacterial strains were isolated from pinyon rhizosphere and screened for biosurfactants production. Among them, six bacterial strains were selected for their potential to produce biosurfactants using two low cost wastes, crude glycerol and lactoserum, as raw material. Both wastes were useful for ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::The aim of this study was to evaluate the effect of ciprofloxacin (CIP), N-acetylcysteine (NAC) alone and in combination on biofilm production and pre-formed mature biofilms on ureteral stent surfaces. Two strains each of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebseilla pneumoniae, Pseud...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The aim of the study was to examine antibacterial activity of the honey of stingless honeybees (Meliponinae). An agar well diffusion assay demonstrated that many honey samples of stingless honeybees inhibited the growth of test strains of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas a...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The aim of this research was to develop a suitable method of succinate dehydrogenase activity assay in situ for different industrial yeast strains. For this purpose different compounds: EDTA, Triton X-100, sodium deoxycholate, digitonin, nystatin and beta-mercaptoethanol were used. The permeabilization process was con...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing P. aeruginosa in a healthcare setting in Alexandria, Egypt. T...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.5604/01.3001.0010.4855
更新日期:2017-09-27 00:00:00
abstract::Bovine mastitis caused by Prototheca spp. can be a disease of high significance because of economic losses and the potential risk to public health. The aim of our study was to evaluate enzymatic activity of Prototheca zopfii. For this study, we used 15 P. zopfii strains previously isolated from cows with clinical and ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2012-09-28 00:00:00
abstract::In vitro studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic Escherichia coli (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory concentrations (MIC) ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2020-003
更新日期:2020-01-01 00:00:00
abstract::The activity of photosynthesizing sulfur bacteria in a continuous culture was studied. The bacteria were isolated from the natural environment with the use of the Winogradski column. Isolated bacteria were cultured in synthetic medium and in the effluent from the column containig HS-. Sulphides, the main product of re...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The aim of this study was to compare the prevalence of EBV genotype and del-LMP-1 in saliva from Polish, Taiwanese and Arabic healthy students. The study group consisted of 56 healthy students; 24 of them Polish, 25 Taiwanese, and 7 Arabic. Typing was carried out using PCR with EBNA-2 primers. A detection of LMP-1 var...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::In this work evaluation of usefulness of monoclonal antibodies (MAbs) CCH2 and AAC10 directed against early--pUL44(DB52) and late--ppUL83(pp65) CMV antigens, utilized in Department of Virology, NIH for routine diagnosis of CMV infection by shell vial and pp65 antigenemia assay, for determination of CMV antigens by flo...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa,...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::The objective of this study was to determine the effect of acid adaptation conditions on heat resistance of E. coli O157: H7 932. E. coli O157: H7 was adapted to acid by exposing the cells to pH 4.5 (2h), pH 5.0 (1 h), and pH 5.5 (1 h) in tryptic soy broth. D and z values of the acid adapted and control cultures at 54...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The antibacterial activities of five antibiotics, three brands of Ofloxacins (Obenasin, Floxavid and Drovid) and two brands of Ciprofloxacins (Uroxin and Siprosan), and five commonly used disinfectants (Lysol, Dettol, Purit, Roberts and Wex-cide) against Staphylococcus aureus, Escherichia coli, Proteus spp., Pseudomon...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The aim of the study was evaluation of qualitative and quantitative changes in bacterial ecosystem in 109 children with inflammatory bowel diseases. Stools obtained from patients were analysed for selected bacteria and concentration of faecal inflammatory markers (calprotectin, lactoferrin, M2-PK). The number of selec...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::The purpose of the study was to evaluate the usefulness of sonication for the diagnosis of prosthetic joint infections (PJIs) by its comparison with periprosthetic tissues (PTs) and synovial fluid (SV-F) cultures. The study groups included 54 patients undergoing exchange of total hip prostheses for so called "aseptic"...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Of 175 Clostridium difficile strains isolated from patient hospitalized in one academic hospital in Warsaw between 2005-2006, one isolate belonged to PCR-ribotype 027/toxinotype III. This isolate had tcdA, tcdB, binary toxin genes (cdtA and cdtB), a 18-bp deletion and a 1 bp deletion at 117 position in the tcdC gene. ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The influence of free base of quinolinilporphyrin and its Zn complex on infectivity of lactophages E3, E5 and E17 has been studied. The results of our investigations show that inhibition of lactophage activity by Zn complex of quinolinilporphyrin at concentration 10 microM and 20 microM was in the range 53-62% and 65-...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::In this research, Salmonella species were isolated from the animal, insect and human enteric sources in Faisalabad, Punjab, Pakistan. These species were characterized by different microbiological and molecular techniques including polymerase chain reaction (PCR) by amplification of the 16S rRNA gene. Furthermore, sequ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.21307/pjm-2019-017
更新日期:2019-01-01 00:00:00
abstract::The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology tec...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Despite the enormous progress in understanding the process of bacterial pathogenesis and interactions of pathogens with eucaryotic cells the infectious diseases still remain the main cause of human premature deaths. It is now recognized that Helicobacter pylori infects about half of the world's population. Based on re...
journal_title:Polish journal of microbiology
pub_type: 杂志文章,评审
doi:
更新日期:2008-01-01 00:00:00