当前位置： SCI文献检索 > JOURNAL OF PERIODONTOLOGY期刊下所有文献 > Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: radiographic observations.

Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: radiographic observations.

Abstract：

BACKGROUND:Platelet-rich plasma (PRP) harbors growth factors identified in bone. It has been suggested that these factors enhance osteogenesis. The objective of this study was to conduct a radiographic evaluation on local bone formation following surgical implantation of a PRP preparation using a critical-size rat calvaria defect model. METHODS:Thirty 22-week-old male Sprague-Dawley rats were used. The PRP preparation was obtained from 10 ml of whole blood drawn from one age-matched donor rat. The preparation was processed by gradient density centrifugation and stored at -80 degrees C until use. Using aseptic techniques, the PRP preparation soak-loaded onto an absorbable collagen sponge (ACS) carrier or ACS alone was surgically implanted into contralateral critical-size 6 mm rat calvaria osteotomies in 18 animals. Twelve animals received ACS alone versus sham surgery in contralateral defects. Animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs were obtained using a standardized protocol. Three masked examiners independently evaluated the radiographic images of the defect sites. Examiner reproducibility was examined by repeat evaluation of all defect sites (r=0.6; P <0.0001). RESULTS:The animals were maintained without adverse events. Defect sites in two animals receiving ACS versus sham surgery (4-week healing interval) were not evaluated due to specimen damage. Seventy-five percent of the sites (PRP/ACS or ACS) exhibited partial closure at 4 weeks; one site (ACS) exhibited full closure without significant differences between protocols (P=0.1797). Fifty percent of the sites receiving PRP/ACS exhibited full closure and 20% partial closure at 8 weeks versus 20% and 80%, respectively, for the ACS control (P=0.7532). There were no noteworthy differences between sites receiving ACS versus sham surgery at 4 or 8 weeks. CONCLUSION:The results suggest that the PRP preparation does not have a significant effect on osteogenesis.

journal_name

J Periodontol

journal_title

Journal of periodontology

authors

Pryor ME,Yang J,Polimeni G,Koo KT,Hartman MJ,Gross H,Agelan A,Manns JM,Wikesjö UM

doi

10.1902/jop.2005.76.8.1287

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

1287-92

issue

eissn

0022-3492

issn

1943-3670

journal_volume

pub_type

杂志文章

在线工具

Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: radiographic observations.