Enhancement of the in vitro transcription by T7 RNA polymerase of short DNA templates containing oxidative thymine lesions.

Abstract:

:5-(Hydroxymethyl)uracil, 5-formyluracil or 5-carboxyuracil can be formed in DNA upon exposure to ionizing radiations. We report the effect of the latter oxidative lesions on the in vitro transcription by T7 RNA polymerase using a single-stranded short model template. Kinetic experiments showed that the transcription yield was increased with the modified template. Due to the unforeseen presence of a hairpin structure within the transcribed RNA, we postulated that we are in the presence of a T7 RNA polymerase pausing signal and that the lesions within this critical DNA structure could interfere with normal regulation of RNA transcription.

journal_name

C R Biol

journal_title

Comptes rendus biologies

authors

Guerniou V,Gasparutto D,Douki T,Cadet J,Sauvaigo S

doi

10.1016/j.crvi.2005.07.003

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

794-801

issue

9

eissn

1631-0691

issn

1768-3238

pii

S1631-0691(05)00130-7

journal_volume

328

pub_type

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