Identification and expression of an atypical isoform of metallothionein in the African clawed frog Xenopus laevis.

Abstract:

:Exploiting the annotation of the western clawed frog Silurana tropicalis genome, we identified a new metallothionein (MT) gene, exhibiting all the features to be considered an active gene, but with an atypical coding region, showing only 17 cysteine residues instead of the canonical 20 cysteines of vertebrate metallothioneins and two anomalous cysteine triplets. However, the presence of a gene in the genome does not ensure its effective expression. By using conventional and Real-Time PCR analyses, we demonstrated that this atypical MT is constitutively expressed throughout the life cycle of the African clawed frog Xenopus laevis; moreover, this gene is highly expressed in the adult liver, the major site of MT expression and synthesis in vertebrates. To our knowledge, the X. laevis MT described in this paper is the first sequence of a vertebrate MT showing only 17 cysteine residues, arranged in two Cys-Cys-Cys motifs. Phylogenetic analyses also demonstrated that the atypical X. laevis MT merges in the anuran clade, but is the most derived sequence among tetrapods MTs. Finally, Tajima's Relative Rate Test suggested a different evolutionary rate between the canonical X. laevis MT and this novel isoform.

journal_name

C R Biol

journal_title

Comptes rendus biologies

authors

Scudiero R,Tussellino M,Carotenuto R

doi

10.1016/j.crvi.2015.03.011

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

314-20

issue

5

eissn

1631-0691

issn

1768-3238

pii

S1631-0691(15)00089-X

journal_volume

338

pub_type

杂志文章
  • Agronomic approach: cropping systems and plant diseases.

    abstract::This paper deals with the effects of agronomic practices on parasite life cycles, and the design of integrated crop protection strategies. Cropping systems have a large effect on the size of the primary inoculum and its localisation, on the development and spread of epidemics, and on the coordination of the life cycle...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章,评审

    doi:10.1016/s1631-0691(03)00006-4

    authors: Meynard JM,Doré T,Lucas P

    更新日期:2003-01-01 00:00:00

  • [Contents of macromineral and trace elements in spirulina (Arthrospira platensis) from France, Chad, Togo, Niger, Mali, Burkina-Faso and Central African Republic].

    abstract::Data on mineral elements in spirulinas being limited, we analyzed macrominerals and trace elements of samples from France and Africa. Spirulinas cultivated in France have a composition in macromineral elements similar to those of the literature. The entire contents of trace elements are low. Unlike marine cyanobacteri...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2013.11.004

    authors: Vicat JP,Doumnang Mbaigane JC,Bellion Y

    更新日期:2014-01-01 00:00:00

  • Marine turtles used to assist Austronesian sailors reaching new islands.

    abstract::Austronesians colonized the islands of Rapa Nui, Hawaii, the Marquesas and Madagascar. All of these islands have been found to harbor Austronesian artifacts and also, all of them are known nesting sites for marine turtles. Turtles are well known for their transoceanic migrations, sometimes totalling thousands of miles...

    journal_title:Comptes rendus biologies

    pub_type: 历史文章,杂志文章

    doi:10.1016/j.crvi.2015.12.001

    authors: Wilmé L,Waeber PO,Ganzhorn JU

    更新日期:2016-02-01 00:00:00

  • Phenotypic variability of natural populations of an invasive drosophilid, Zaprionus indianus, on different continents: comparison of wild-living and laboratory-grown flies.

    abstract::Phenotypic variability in nature is the most important feature for Darwinian adaptation, yet it has been rarely investigated in invasive species. Zaprionus indianus is an Afrotropical drosophilid species that have recently invaded the Palearctic and the Neotropical regions. Here, we compared the variability of three s...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2009.06.003

    authors: Yassin A,David JR,Bitner-Mathé BC

    更新日期:2009-10-01 00:00:00

  • Discrimination through silk recognition: the case of the two-spotted spider mite Tetranychus urticae.

    abstract::Choosing a suitable habitat is a main step in the settlement process, particularly for species having weak movement abilities. Reliable cues are thus needed for habitat selection. In silk-spinning arthropods, silk can be used as a social cue to select an appropriate location. Silk can also provide information on the p...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2012.07.001

    authors: Clotuche G,Deneubourg JL,Mailleux AC,Detrain C,Hance T

    更新日期:2012-08-01 00:00:00

  • The rise of genomics.

    abstract::A brief history of the development of genomics is provided. Complete sequencing of genomes of uni- and multicellular organisms is based on important progress in sequencing and bioinformatics. Evolution of these methods is ongoing and has triggered an explosion in data production and analysis. Initial analyses focused ...

    journal_title:Comptes rendus biologies

    pub_type: 历史文章,杂志文章,评审

    doi:10.1016/j.crvi.2016.05.002

    authors: Weissenbach J

    更新日期:2016-07-01 00:00:00

  • Further support for the clades obtained by multiple molecular phylogenies in the acanthomorph bush.

    abstract::Several recent molecular studies have begun to clarify the phylogeny of Acanthomorpha (Teleostei), a wide clade of teleost fishes. However, different molecular datasets do not agree on a single history of the taxa, probably because of marker-specific biases. The 'total-evidence' approach maximizes character congruence...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2005.04.002

    authors: Dettai A,Lecointre G

    更新日期:2005-07-01 00:00:00

  • Genetic and epigenetic variation of human populations: An adaptive tale.

    abstract::The evolutionary history of modern humans means much more than their demographic past. It includes the way in which humans have had to genetically adapt to the different environments they have encountered-nutritional, climatic or pathogenic-as well as the different epigenetic responses elicited by such environmental c...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章,评审

    doi:10.1016/j.crvi.2016.04.005

    authors: Quintana-Murci L

    更新日期:2016-07-01 00:00:00

  • [Storage proteins from seeds of Pinus pinea L].

    abstract::The Mediterranean stone pine Pinus pinea L. (gymnosperm, Pinaceae) is much appreciated for its seed production, widely used in food preparation in the Mediterranean Basin. Seeds contain 25% proteins on a dry-weight basis. Pinus pinea accumulate globulins as major storage proteins in seeds (75% of total storage protein...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2007.03.011

    authors: Nasri N,Triki S

    更新日期:2007-05-01 00:00:00

  • A glimpse of lignicolous marine fungi occurring in coastal water bodies of Tamil Nadu (India).

    abstract::In the present investigation, a total of 51 marine fungi were obtained from wood samples collected from four locations of Tamil Nadu (Tuthukudi, Chennai, Kanyakumari and Pichavaram), India. Out of these 51, 28 were ascomycetes, one was basidiomycete and 22 were mitosporic fungi. Maximum fungal diversity was encountere...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2008.03.008

    authors: Nambiar GR,Raveendran K,Changxing Z,Jaleel CA

    更新日期:2008-06-01 00:00:00

  • ISSR marker-assisted genetic diversity analysis of Dioscorea hispida and selection of the best variety for sustainable production.

    abstract::Plant breeding is a way of selection of a particular individual for the production of the progeny by separating or combining desired characteristics. The objective of this study was to justify different characteristics of Dioscorea hispida (Ubi gadong) varieties using molecular techniques to select the best variety fo...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2017.08.003

    authors: Nudin NFH,Ali AM,Ngah N,Mazlan NZ,Mat N,Ghani MNA,Alias N,Zakaria AJ,Jahan MS

    更新日期:2017-08-01 00:00:00

  • The melanocortins and melanin-concentrating hormone in the central regulation of feeding behavior and energy homeostasis.

    abstract::A number of different neuropeptides exert powerful concerted controls on feeding behavior and energy balance, most of them being produced in hypothalamic neuronal networks under stimulation by anabolic and catabolic peripheral hormones such as ghrelin and leptin, respectively. These peptide-expressing neurons intercon...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章,评审

    doi:10.1016/j.crvi.2006.03.021

    authors: Nahon JL

    更新日期:2006-08-01 00:00:00

  • Production of a high level of laccase by submerged fermentation at 120-L scale of Cerrena unicolor C-139 grown on wheat bran.

    abstract::Submerged fermentation in a stirred bioreactor of the white rot fungus Cerrena unicolor C-139 was done at a 120-L scale in the presence of wheat bran as a cheap lignocellulosic substrate for fungus growth and laccase production. Enzyme monitoring showed that laccase production started after 2 days of cultivation, atta...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2014.12.001

    authors: Songulashvili G,Spindler D,Jimenéz-Tobón GA,Jaspers C,Kerns G,Penninckx MJ

    更新日期:2015-02-01 00:00:00

  • Chromium stress in paddy: (i) nutrient status of paddy under chromium stress; (ii) phytoremediation of chromium by aquatic and terrestrial weeds.

    abstract::A field experiment was conducted with paddy (Oryza sativa L.) irrigated with different concentrations (Control, 2.5, 5, 10, 25, 50, 75, 100 and 200 mg/l) of chromium. The changes in growth, yield, nutrient content and chromium accumulation in the paddy are reported. The growth of shoot, root, total leaf area, fresh we...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2010.03.002

    authors: Sundaramoorthy P,Chidambaram A,Ganesh KS,Unnikannan P,Baskaran L

    更新日期:2010-08-01 00:00:00

  • Learning capacities and welfare in an Antillean manatee, Trichechus manatus manatus.

    abstract::Studies on the cognitive abilities of manatees are limited despite their importance for the environmental enrichment and welfare of individuals in captivity and the understanding of manatee behaviour in the wild. Our study analyses how the presence of new stimuli and their association with food may have changed the be...

    journal_title:Comptes rendus biologies

    pub_type: 新闻

    doi:10.5802/crbiol.6

    authors: Hénaut Y,Lara-Sánchez LE,Morales-Vela B,Machkour-M'Rabet S

    更新日期:2020-06-05 00:00:00

  • Evaluation of the genetic trend of milk yield in the multiple ovulation and embryo transfer populations of dairy cows, using stochastic simulation.

    abstract::Stochastic modeling of dairy cattle populations using multiple ovulation and embryo transfer (MOET) was used to compare 15-year genetic responses with an artificial insemination (AI) program. MOET and AI techniques were simulated in four populations, two with 100 breeding females each and two with 400 breeding females...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2010.07.001

    authors: Hossein-Zadeh NG

    更新日期:2010-10-01 00:00:00

  • [Salinity effect on germination, growth, and grain production of some autochthonous pear millet ecotypes (Pennisetum glaucum (L.) R. Br.)].

    abstract::This study compared the behaviour of six autochthonous pear millet ecotypes collected through the Tunisian territory under salt stress from germination to maturity. It showed that salt has little effect on germination rate and coleoptile emergence. However, this effect is more significant for radicular growth and betw...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2008.01.006

    authors: Radhouane L

    更新日期:2008-04-01 00:00:00

  • Studies of fertility and fecundity: comparative approaches from demography and epidemiology.

    abstract::The approaches used by demographers and by epidemiologists for studying the fecundity of couples (i.e. their ability to conceive) have converged, whereas they were historically divergent. Demography traditionally focused on fertility - the circumstances in which livebirths occur - with the aim of predicting population...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章,评审

    doi:10.1016/j.crvi.2007.02.013

    authors: Leridon H

    更新日期:2007-04-01 00:00:00

  • Phylogenetic relationships and biodiversity in Hylids (Anura: Hylidae) from French Guiana.

    abstract::We evaluated two biodiversity criteria, higher taxonomic diversity and phylogenetic diversity in French Guiana. For this, we used a recent assessment of the knowledge accumulated since 30 years of study on the amphibian species currently known in French Guiana. We focused on two well-represented genera, Hyla and Scina...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2005.07.005

    authors: Salducci MD,Marty C,Fouquet A,Gilles A

    更新日期:2005-10-01 00:00:00

  • [A new species of wild mice on the Island of Cyprus].

    abstract::A mitochondrial and nuclear gene analysis allowed us to precise the taxonomical position of the two sympatric species of mice known to be present on Cyprus. One of them is the commensal house mouse M. m. domesticus, and the other revealed to be a new taxon that is a sister species of M. spicilegus and M. macedonicus. ...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2004.03.001

    authors: Bonhomme F,Orth A,Cucchi T,Hadjisterkotis E,Vigne JD,Auffray JC

    更新日期:2004-05-01 00:00:00

  • Spatial distribution of pH and organic matter in urban soils and its implications on site-specific land uses in Xuzhou, China.

    abstract::The spatial variation of soil pH and soil organic matter (SOM) in the urban area of Xuzhou, China, was investigated in this study. Conventional statistics, geostatistics, and a geographical information system (GIS) were used to produce spatial distribution maps and to provide information about land use types. A total ...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2014.02.008

    authors: Mao Y,Sang S,Liu S,Jia J

    更新日期:2014-05-01 00:00:00

  • Sexual selection: Another Darwinian process.

    abstract::Why was sexual selection so important to Darwin? And why was it de-emphasized by almost all of Darwin's followers until the second half of the 20th century? These two questions shed light on the complexity of the scientific tradition named "Darwinism". Darwin's interest in sexual selection was almost as old as his dis...

    journal_title:Comptes rendus biologies

    pub_type: 传,历史文章,杂志文章

    doi:10.1016/j.crvi.2009.12.001

    authors: Gayon J

    更新日期:2010-02-01 00:00:00

  • Characterization of P5CS gene in Calotropis procera plant from the de novo assembled transcriptome contigs of the high-throughput sequencing dataset.

    abstract::The wild plant known as Calotropis procera is important in medicine, industry and ornamental fields. Due to spread in areas that suffer from environmental stress, it has a large number of tolerance genes to environmental stress such as drought and salinity. Proline is one of the most compatible solutes that accumulate...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2014.09.002

    authors: Ramadan AM,Hassanein SE

    更新日期:2014-12-01 00:00:00

  • Separating objects and 'neural' computation.

    abstract::This article demonstrates how that the effectiveness of nervous system in doing the computations essential to an organism can be based on using algorithms that are readily implemented by nervous system 'device biophysics'. Collective effects and collective algorithms that exploit their dynamics provide powerful potent...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章,评审

    doi:10.1016/s1631-0691(03)00017-9

    authors: Hopfield JJ,Brody CD

    更新日期:2003-02-01 00:00:00

  • Enhancement of the in vitro transcription by T7 RNA polymerase of short DNA templates containing oxidative thymine lesions.

    abstract::5-(Hydroxymethyl)uracil, 5-formyluracil or 5-carboxyuracil can be formed in DNA upon exposure to ionizing radiations. We report the effect of the latter oxidative lesions on the in vitro transcription by T7 RNA polymerase using a single-stranded short model template. Kinetic experiments showed that the transcription y...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2005.07.003

    authors: Guerniou V,Gasparutto D,Douki T,Cadet J,Sauvaigo S

    更新日期:2005-09-01 00:00:00

  • [Reproductive behavior of the green birdmouth wrasse Gomphosus caeruleus on a Reunion Island reef: Mode of reproduction, environmental factors and reproductive strategy alternation].

    abstract::The green birdmouth wrasse Gomphosus caeruleus is present all year round on the coral reefs of Reunion Island (Indian Ocean). A group of individuals was followed on one of these reefs with the objective of studying the reproduction mode of the species, the influence of environmental factors, and social behaviors on th...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2017.11.004

    authors: Desvignes T,Bourjon P,Chanet B

    更新日期:2018-01-01 00:00:00

  • [Microphytobenthos assemblage mapping by spatial visible-infrared remote sensing in a shellfish ecosystem].

    abstract::The aim of this work is to assess the use of (SPOT) multispectral visible infrared remote sensing to study microphytobentos assemblages in a shellfish ecosystem (Bay of Bourgneuf, France). SPOT satellite images (acquired at low tide in spring or autumn between 1986 and 1998) were calibrated using in situ radiometric d...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/s1631-0691(03)00125-2

    authors: Méléder V,Launeau P,Barillé L,Rincé Y

    更新日期:2003-04-01 00:00:00

  • Investigation of the ultrastructure of Dendrocoelum constrictum (Platyhelminthes, Tricladida) spermatogenesis and mature spermatozoa.

    abstract::To add to our understanding of dendrocoelid spermatozoa and to describe additional phylogenetic characters, the ultrastructure of the testis was investigated in the subterranean freshwater planarian Dendrocoelum constrictum. This is the first study investigating spermatogenesis and spermatozoon ultrastructure in a sub...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2014.07.004

    authors: Harrath AH,Gammoudi M,Mansour L,Ahmed M,Sirotkin AV,Al Omar SY,Ibrahim KE,Alwasel SH

    更新日期:2014-09-01 00:00:00

  • Toxicity assessment of the puffer fish Lagocephalus lagocephalus from the Tunisian coast.

    abstract::This study was undertaken to assess the risk of poisoning due to consumption of the puffer fish Lagocephalus lagocephalus collected along the Tunisian coast. Wistar rats were daily intraperitoneally injected, for 10 days, with acidic extracts of liver or flesh (muscles + skin) of L. lagocephalus. Control rats received...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2008.05.005

    authors: Saoudi M,Abdelmouleh A,Kammoun W,Ellouze F,Jamoussi K,El Feki A

    更新日期:2008-08-01 00:00:00

  • Identification of new molecules extracted from Quercus suber L. cork.

    abstract::Various methods of suberin extraction have been used in order to identify monomers of this complex polymer. Pre-extraction of waxes has allowed us to identify for the first time 3-friedelanol as a terpen from cork. Moreover, the wax chemical composition found here varied from previous results since cerin was not ident...

    journal_title:Comptes rendus biologies

    pub_type: 杂志文章

    doi:10.1016/j.crvi.2008.08.011

    authors: Coquet C,Ferré E,Peyronel D,Dal Farra C,Farnet AM

    更新日期:2008-11-01 00:00:00