Abstract：
:Submerged fermentation in a stirred bioreactor of the white rot fungus Cerrena unicolor C-139 was done at a 120-L scale in the presence of wheat bran as a cheap lignocellulosic substrate for fungus growth and laccase production. Enzyme monitoring showed that laccase production started after 2 days of cultivation, attaining a maximum activity of 416.4 U·mL(-1) at day 12 of fermentation. After treatment of culture liquid by successive micro- and ultrafiltration (5kDa), a liquid concentrate containing 22203176 units of laccase was obtained. Obtaining large amount of laccase is essential for various industrial applications, including detoxification of industrial effluents, textile and petrochemical industries, polymer synthesis, bioremediation of contaminated area, stabilization of beverages, production of cosmetics, manufacture of anti-cancer drugs, and nanobiotechnology. The cultivation method and the fungal strain used here provided a substantial amount of enzyme produced at a price lower than 0.01 € cent/unit enzyme.
journal_name
C R Bioljournal_title
Comptes rendus biologiesauthors
Songulashvili G,Spindler D,Jimenéz-Tobón GA,Jaspers C,Kerns G,Penninckx MJdoi
10.1016/j.crvi.2014.12.001subject
Has Abstractpub_date
2015-02-01 00:00:00pages
121-5issue
2eissn
1631-0691issn
1768-3238pii
S1631-0691(14)00304-7journal_volume
338pub_type
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