Rapid protein identification using monolithic enzymatic microreactor and LC-ESI-MS/MS.

abstract：:Immune proteomics is an increasingly powerful tool for the investigation of the adaptive immune response to natural encounters between micro-organisms and their hosts. The versatile species Staphylococcus aureus serves to illustrate how these techniques can be employed to appreciate the complexity and diversity of the...

journal_title：Proteomics

authors： Bröker BM,van Belkum A

更新日期：2011-08-01 00:00:00

abstract：:The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...

journal_title：Proteomics

authors： Lubelwana Hafver T,Wanichawan P,Manfra O,de Souza GA,Lunde M,Martinsen M,Louch WE,Sejersted OM,Carlson CR

更新日期：2017-09-01 00:00:00

abstract：:Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...

journal_title：Proteomics

authors： Castagna A,Antonioli P,Astner H,Hamdan M,Righetti SC,Perego P,Zunino F,Righetti PG

更新日期：2004-10-01 00:00:00

abstract：:The transfection of glycoprotein glycosyltransferase genes into cells leads to modification of both the structure and function of the glycoproteins and as a result, changes in glycome patterns. N-glycan branching enzymes hold some promise as a model system for the identification of glycome patterns. Both N-acetylgluco...

journal_title：Proteomics

authors： Taniguchi N,Ekuni A,Ko JH,Miyoshi E,Ikeda Y,Ihara Y,Nishikawa A,Honke K,Takahashi M

更新日期：2001-02-01 00:00:00

abstract：:The last years have seen the emergence of many large-scale proteomics initiatives that have identified thousands of new protein interactions and macromolecular assemblies. However, unfortunately, only a few among the discovered complexes meet the high-quality standards required to be promptly used in structural studie...

journal_title：Proteomics

authors： Pache RA,Aloy P

更新日期：2008-05-01 00:00:00

abstract：:CREC proteins constitute a family of EF-hand calcium binding proteins localized to the secretory pathway. Calumenin is the only member known to be secreted. Recently, it was shown that thrombin-activated thrombocytes liberate calumenin, which also is found in atherosclerotic lesions but not in normal vasculature. To s...

journal_title：Proteomics

authors： Ostergaard M,Hansen GA,Vorum H,Honoré B

更新日期：2006-06-01 00:00:00

abstract：:SARS-CoV-2 infection results in a mild-to-moderate disease course in most patients, allowing outpatient self-care and quarantine. However, in approx. 10% of cases a two- or three-phasic critical disease course with starting from day 7 to 10 is observed. To facilitate and plan outpatient care, biomarkers prognosing suc...

journal_title：Proteomics

authors： Wendt R,Kalbitz S,Lübbert C,Kellner N,Macholz M,Schroth S,Ermisch J,Latosisnka A,Arnold B,Mischak H,Beige J,Metzger J

更新日期：2020-09-10 00:00:00

abstract：:The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...

journal_title：Proteomics

authors： Schaumburg J,Diekmann O,Hagendorff P,Bergmann S,Rohde M,Hammerschmidt S,Jänsch L,Wehland J,Kärst U

更新日期：2004-10-01 00:00:00

abstract：:CD28 delivers a co-stimulatory signal for T cell antigen receptor induced activation of T cells through a mechanism which remains mostly elusive to date. In order to try and gain insight into CD28 function, we therefore applied state-of-the-art mass spectrometric protein identification technology to the analysis of CD...

journal_title：Proteomics

authors： Heller M,Watts JD,Aebersold R

更新日期：2001-01-01 00:00:00

abstract：:Urinary microvesicles constitute a rich source of membrane-bound and intracellular proteins that may provide important clues of pathophysiological mechanisms in renal disease. In the current study, we analyzed and compared the proteome of urinary microvesicles from patients with idiopathic membranous nephropathy (iMN)...

journal_title：Proteomics

authors： Rood IM,Merchant ML,Wilkey DW,Zhang T,Zabrouskov V,van der Vlag J,Dijkman HB,Willemsen BK,Wetzels JF,Klein JB,Deegens JK

更新日期：2015-11-01 00:00:00

abstract：:Over the past decade, considerable progress has been made with respect to the analytical methods for analysis of glycans from biological sources. Regardless of the specific methods that are used, glycan analysis includes isolation, identification, and quantitation. Derivatization is indispensable to increase their ide...

journal_title：Proteomics

authors： Yang S,Rubin A,Eshghi ST,Zhang H

更新日期：2016-01-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. Miniaturization of IMAC column is essential for the analysis of a small amount of sample. Nanoscale IMAC column was prepared by chemical modification of...

journal_title：Proteomics

authors： Feng S,Pan C,Jiang X,Xu S,Zhou H,Ye M,Zou H

更新日期：2007-02-01 00:00:00

abstract：:Sirtuins are a family of NAD(+)-dependent protein deacetylases that regulate cellular functions through deacetylation of a wide range of protein targets. Overexpression of Sir2, the first gene discovered in this family, is able to extend the life span in various organisms. The anti-aging effects of human homologues of...

journal_title：Proteomics

authors： Law IK,Liu L,Xu A,Lam KS,Vanhoutte PM,Che CM,Leung PT,Wang Y

更新日期：2009-05-01 00:00:00

abstract：:Simple protein separation by 1DE is a widely used method to reduce sample complexity and to prepare proteins for mass spectrometric identification via in-gel digestion. While several automated solutions are available for in-gel digestion particularly of small cylindric gel plugs derived from 2D gels, the processing of...

journal_title：Proteomics

authors： Schmidt C,Hesse D,Raabe M,Urlaub H,Jahn O

更新日期：2013-05-01 00:00:00

abstract：:The current treatment for glioblastoma includes temozolomide (TMZ) chemotherapy, yet the mechanism of action of TMZ is not thoroughly understood. Here, we investigated the TMZ-induced changes in the proteome of the glioma-derived cell line (U251) by 2D DIGE. We found 95 protein spots to be significantly altered in the...

journal_title：Proteomics

authors： Kumar DM,Patil V,Ramachandran B,Nila MV,Dharmalingam K,Somasundaram K

更新日期：2013-07-01 00:00:00

abstract：:Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...

journal_title：Proteomics

authors： Westers L,Westers H,Zanen G,Antelmann H,Hecker M,Noone D,Devine KM,van Dijl JM,Quax WJ

更新日期：2008-07-01 00:00:00

abstract：:A proteomics approach has been used to study changes in protein abundance during leaf senescence in white clover. Changes in cell ultrastructure were also examined using transmission electron microscopy. The most obvious ultrastructural changes during senescence occurred in chloroplasts, with progressive loss of thyla...

journal_title：Proteomics

authors： Wilson KA,McManus MT,Gordon ME,Jordan TW

更新日期：2002-09-01 00:00:00

abstract：:MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...

journal_title：Proteomics

authors： Niggeweg R,Köcher T,Gentzel M,Buscaino A,Taipale M,Akhtar A,Wilm M

更新日期：2006-01-01 00:00:00

abstract：:Separation and identification of hydrophobic membrane proteins is a major challenge in proteomics. Identification of such sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins by peptide mass fingerprinting (PMF) via matrix-assisted laser desorption/ionization-time of flight (MALDI-TO...

journal_title：Proteomics

authors： Zischka H,Gloeckner CJ,Klein C,Willmann S,Swiatek-de Lange M,Ueffing M

更新日期：2004-12-01 00:00:00

abstract：:Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous seru...

journal_title：Proteomics

authors： Bijttebier J,Tilleman K,Dhaenens M,Deforce D,Van Soom A,Maes D

更新日期：2009-10-01 00:00:00

abstract：:Entamoeba histolytica is known for its extraordinary capacity to destroy human tissues, leading to invasive diseases such as ulcerative colitis or extra-intestinal abscesses. In order to identify the virulence factors of this parasite phenotypes and proteomes of two recently identified genetically related cell lines (...

journal_title：Proteomics

authors： Biller L,Schmidt H,Krause E,Gelhaus C,Matthiesen J,Handal G,Lotter H,Janssen O,Tannich E,Bruchhaus I

更新日期：2009-09-01 00:00:00

abstract：:Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

更新日期：2009-07-01 00:00:00

abstract：:Proteome analysis of human hepatocellular carcinoma tissues was conducted using two-dimensional difference gel electrophoresis coupled with mass spectrometry. Paired samples from the normal and tumor region of resected human liver were labeled with Cy3 and Cy5, respectively while the pooled standard sample was labeled...

journal_title：Proteomics

authors： Liang CR,Leow CK,Neo JC,Tan GS,Lo SL,Lim JW,Seow TK,Lai PB,Chung MC

更新日期：2005-05-01 00:00:00

abstract：:Plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. Some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergisti...

journal_title：Proteomics

authors： da Silva AJ,Gómez-Mendoza DP,Junqueira M,Domont GB,Ximenes Ferreira Filho E,de Sousa MV,Ricart CA

更新日期：2012-08-01 00:00:00

abstract：:Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...

journal_title：Proteomics

authors： Erce MA,Pang CN,Hart-Smith G,Wilkins MR

更新日期：2012-02-01 00:00:00

abstract：:Clostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate-active enzymes were identified, and their modular structures and activities ...

journal_title：Proteomics

authors： Blouzard JC,Coutinho PM,Fierobe HP,Henrissat B,Lignon S,Tardif C,Pagès S,de Philip P

更新日期：2010-02-01 00:00:00

abstract：:Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...

journal_title：Proteomics

authors： Chardonnet S,Decottignies P,Amar L,Le Caer JP,Davis S,Laroche S,Le Maréchal P

更新日期：2007-01-01 00:00:00

abstract：:Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated ...

journal_title：Proteomics

authors： Pietsch J,Sickmann A,Weber G,Bauer J,Egli M,Wildgruber R,Infanger M,Grimm D

更新日期：2012-08-01 00:00:00

abstract：:CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...

journal_title：Proteomics

authors： Kim MS,Zhong J,Kandasamy K,Delanghe B,Pandey A

更新日期：2011-06-01 00:00:00

abstract：:Previously, we reported that nucleophosmin (NPM) was increased in glioblastoma multiforme (GBM). NPM is a phosphoprotein related to apoptosis, ribosome biogenesis, mitosis, and DNA repair, but details about its function remain unclear. We treated U87MG and A172 cells with small interference RNA (siRNA) and obtained a ...

journal_title：Proteomics

authors： Gimenez M,Marie SK,Oba-Shinjo SM,Uno M,da Silva R,Laure HJ,Izumi C,Otake A,Chammas R,Rosa JC

更新日期：2012-08-01 00:00:00