Abstract:
:We have generated hybridomas which secrete monoclonal antibodies to the AT1 subtype of the angiotensin II receptor (AT1 receptor). These were obtained after immunization of Balb C/c mice with synthetic peptides representing sequences from either the extracellular domain (residues 8-17) or the intracellular domain (residues 229-237) of the AT1 receptor. Hybridoma populations were first screened for the production of antibodies which bound to rat liver cells. Further selection, and cloning by limiting dilution, was carried out for antibodies which bound specifically to rat adrenal glomerulosa cells. Confirmation that the antibody designated 6313/G2 interacted with the angiotensin II receptor was obtained using COS-7 cells transfected with AT1A receptor cDNA. In particular, the initial characterization of 6313/G2 showed specific immunofluorescence of vascular endothelium.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Barker S,Marchant W,Ho MM,Puddefoot JR,Hinson JP,Clark AJ,Vinson GPdoi
10.1677/jme.0.0110241subject
Has Abstractpub_date
1993-10-01 00:00:00pages
241-5issue
2eissn
0952-5041issn
1479-6813journal_volume
11pub_type
杂志文章abstract::Activation of the hypothalamo-pituitary-adrenal (HPA) axis during stress is associated with increased expression of genes that code for regulatory hormones such as corticotrophin-releasing factor (CRF) and ACTH. The identity of the transcription factors that mediate these changes in gene expression is not known. In th...
journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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abstract::Estrogen plays an important role in many physiological events including carcinogenesis and the development of human breast cancer. However, the molecular mechanisms of estrogen signaling in cancers have not been clarified hitherto and accurate therapeutic prediction of breast cancer is earnestly desired. We first carr...
journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章,评审
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章,评审
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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更新日期:2004-04-01 00:00:00
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Journal of molecular endocrinology
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更新日期:2004-02-01 00:00:00
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章
doi:10.1677/jme.0.0120313
更新日期:1994-06-01 00:00:00
abstract::The angiotensin II (Ang II) type 2 receptor (AT(2)) is a member of the seven-transmembrane domain, G-protein coupled receptor family. This receptor is ubiquitously distributed in the fetus but, in most tIssues, its expression dramatically falls in the first few hours after birth. Based on this observation, the hypothe...
journal_title:Journal of molecular endocrinology
pub_type: 杂志文章,评审
doi:10.1677/jme.0.0310359
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章
doi:10.1677/jme.0.0080249
更新日期:1992-06-01 00:00:00
abstract::During the process of insulitis in the pathogenesis of type I (insulin-dependent) diabetes mellitus, proinflammatory cytokines induce expression of the death receptor Fas on the surface of pancreatic beta-cells and thereby contribute to the enhanced susceptibility of beta-cells for apoptosis. The aim of this study was...
journal_title:Journal of molecular endocrinology
pub_type: 杂志文章
doi:10.1677/jme.0.0300163
更新日期:2003-04-01 00:00:00
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章,评审
doi:10.1677/jme.1.01600
更新日期:2004-12-01 00:00:00
abstract::The crystal structures of the leucine-rich repeat domain (LRD) of the FSH receptor (FSHR) in complex with FSH and the TSH receptor (TSHR) LRD in complex with the thyroid-stimulating autoantibody (M22) provide opportunities to assess the molecular basis of the specificity of glycoprotein hormone-receptor binding. A com...
journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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更新日期:1990-04-01 00:00:00
abstract::Transportation of vitamin C [also called ascorbic acid (AA)], an important water-soluble antioxidant and cofactor in testis, requires glucose transporter family (GLUTs) and sodium/vitamin C cotransporter family (SVCT1 and SVCT2). There is so far scant information vis-à-vis the functional roles of SVCTs in testis, alth...
journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
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更新日期:1993-06-01 00:00:00
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journal_title:Journal of molecular endocrinology
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:Journal of molecular endocrinology
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:Journal of molecular endocrinology
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