Abstract：
:Barrett's oesophagus (BO) and oesophageal adenocarcinoma (OAC) are regarded as complications of gastro-oesophageal reflux disease, although all the factors that contribute to the development of these lesions are unknown. Acid suppressive drugs are widely used for symptomatic therapy of reflux disease but may induce hypersecretion of gastrin peptides. Amidated gastrin (G-17) has been shown to be a growth factor for OAC cells. We have examined the effects of glycine-extended gastrin (G-Gly), an alternative product of progastrin processing on apoptosis in the QhERT Barrett's oesophageal cell line and OE33 and BIC-1 OAC cells. G-Gly inhibited serum-starvation and camptothecin-induced apoptosis in all three cell lines, G-17 was only effective in OE33 cells. By contrast to the effects of G-17, the anti-apoptotic effect of G-Gly was independent of both the CCK(2) receptor and cyclo-oxygenase-2 activity. G-Gly stimulated JAK2 phosphorylation and kinase activity and JAK2-dependent STAT3 phosphorylation and transcriptional activity. G-Gly also increased mRNA and protein levels of the anti-apoptotic proteins survivin and BCL2L1 but did not affect the levels of BAD, BAX or BCL-2. Novel small molecule inhibitors of JAK2 and STAT3 as well as STAT3 siRNA blocked the anti-apoptotic effects of G-Gly and inhibited the induction of survivin and BCL2L1 in OE33 cells. We conclude that G-Gly inhibits apoptosis in BO and OAC via mechanisms distinct from those activated by G-17 and involving JAK2 and STAT3 activation. Release of gastrin peptides in response to acid suppressive therapy may adversely influence the dynamics of the epithelium in BO.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Beales IL,Ogunwobi OOdoi
10.1677/JME-08-0096subject
Has Abstractpub_date
2009-04-01 00:00:00pages
305-18issue
4eissn
0952-5041issn
1479-6813pii
JME-08-0096journal_volume
42pub_type
杂志文章abstract：:Several hormone agonists exert their physiological actions by triggering an inositol phospholipid-Ca2+ signalling cascade and cytosolic alkalinization. Although calcium ionophores have been used extensively to probe the role of Ca2+ in the regulation of steroidogenesis in granulosa cells, the precise relationship betw...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0090001
更新日期：1992-08-01 00:00:00
abstract：:Studies were performed to determine whether ARP-1, which is an orphan receptor of the steroid receptor superfamily, inhibits basal activity of the human placental lactogen (hPL) promoter and the increase in hPL promoter activity in response to the receptors for thyroid hormone (TR) and retinoic acid (RAR). Co-transfec...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0160221
更新日期：1996-06-01 00:00:00
abstract：:Whether insulin, at physiological concentrations, has direct effects on vascular smooth muscle cells (VSMCs) remains controversial. Our aim was to characterize the mechanism for insulin resistance in VSMCs. For comparison, the effects of IGF1 and IGF2 were also studied. Cultured human aortic smooth muscle cells (HASMC...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-09-0021
更新日期：2009-12-01 00:00:00
abstract：:Sucrose density gradient purified plasma membranes isolated from brown adipose tissue of cold-acclimated hamsters (4-10 weeks at 0-4 degreesC) were analysed for the content of the short (GsalphaS) and long (GsalphaL) variants of Gsalpha protein (the alpha subunit of the stimulatory G protein) and compared with the mem...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0220055
更新日期：1999-02-01 00:00:00
abstract：:Cardiovascular disease (CVD) remains the leading cause of death worldwide. Low levels of vitamin D are associated with high risk of myocardial infarction, even after controlling for factors associated with coronary artery disease. A growing body of evidence indicates that vitamin D plays an important role in CVD-relat...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-14-0168
更新日期：2014-12-01 00:00:00
abstract：:Urocortin (Ucn), a corticotropin-releasing factor (CRF)-related neuropeptide binding both CRF type 1 receptor (CRFR1) and CRFR2, has recently been found in prostate cancer. However, no report has yet been known to elucidate the roles of Ucn in prostate cancer via the two receptors. In this study, the expression of bot...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-11-0048
更新日期：2011-09-07 00:00:00
abstract：:This study developed a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method to measure estrogen receptor-alpha (ERalpha) mRNA in the rainbow trout (Oncorhynchus mykiss). Using RT-PCR, and primers based on the known ERalpha DNA sequence in this species, cDNA sequences representing most of the pr...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0250243
更新日期：2000-10-01 00:00:00
abstract：:The contribution of hypothalamic appetite-regulating peptides to further hyperphagia accompanying the course of lactation in rats was investigated by using PCR array and real-time PCR. Furthermore, changes in the mRNA expression for appetite-regulating peptides in the hypothalamic arcuate nucleus (ARC) were analyzed a...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-13-0015
更新日期：2014-01-30 00:00:00
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journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0300163
更新日期：2003-04-01 00:00:00
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journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1677/jme.0.0060121
更新日期：1991-04-01 00:00:00
abstract：:Decreased suppressors of cytokine signaling (SOCS) activity in human gestational tissues may play a part in the onset/progression of term labor. Since SOCS proteins negatively regulate cytokine-mediated inflammatory processes, we hypothesized that SOCS proteins are elevated in gestational tissues from spontaneous pret...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01767
更新日期：2005-08-01 00:00:00
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journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0130187
更新日期：1994-10-01 00:00:00
abstract：:DNA encoding the N-terminal 415 residues of the human thyrotrophin receptor (predicted to code for the large extracellular region) was introduced into Chinese hamster ovary (CHO) cells using the glutamine synthetase/cytomegalovirus amplifiable expression system, and into E. coli using the pGEX-3X expression vector. Su...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0090227
更新日期：1992-12-01 00:00:00
abstract：:Successful human embryo implantation requires a proper differentiation of endometrial stromal cells (ESCs) into decidual cells, during a process called decidualization. ESCs express specific molecules, such as prolactin, insulin-like growth factor-binding protein-1 (IGFBP-1) and connexin-43. Decidual cells are also in...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-17-0046
更新日期：2017-10-01 00:00:00
abstract：:Evidence indicates that central regulation of food intake is well conserved along the vertebrate lineage, at least between teleost fish and mammals. However, several differences arise in the comparison between both groups. In this review, we describe similarities and differences between teleost fish and mammals on an ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-17-0320
更新日期：2018-05-01 00:00:00
abstract：:Non-steroidal antioestrogens, such as tamoxifen, inhibit the growth of human breast cancer cells. The experiments described here compare and contrast the efficacy of tamoxifen and the 'pure' antioestrogen, ICI 164384, on the inhibition of proliferation of MCF-7 cells. Previous studies have shown that ICI 164384 has a ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0020225
更新日期：1989-05-01 00:00:00
abstract：:Luteotropic glycoprotein hormones (LGH) include luteinizing hormone (LH) and chorionic gonadotropin (CG). The order Primates is the only phylogenetic clad known to exhibit more than one LGHbeta subunit gene per haploid genome. In the present study, we report the discovery of a second case of LGHbeta gene replication, ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0210019
更新日期：1998-08-01 00:00:00
abstract：:This study determined whether twice-daily intrauterine injections of ovine conceptus secretory proteins (oCSP) containing type-I trophoblast interferon (25 micrograms/uterine horn) from day 11 to day 15 post-oestrus (oestrus = day 0) could alter the binding capacities of endometrial receptors for oxytocin, progesteron...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0100185
更新日期：1993-04-01 00:00:00
abstract：:The effect of steroid hormone treatment on coho salmon (Oncorhynchus kisutch) was examined. The cDNAs for coho salmon FSH beta and LH beta subunits were cloned and sequenced using reverse transcriptase PCR. Northern blot analysis revealed that a single transcript of 1 kb for each of these subunits was present in the p...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0210291
更新日期：1998-12-01 00:00:00
abstract：:Atrial natriuretic peptide (ANP) as well as its receptors is found in mammalian ovary and follicular cells and its function in oocyte meiotic maturation has also been reported in Xenopus, hamster and rat. But the results are controversial and the physiological mechanism of ANP on oocyte maturation is not clear, especi...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01673
更新日期：2005-04-01 00:00:00
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journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-16-0116
更新日期：2016-11-01 00:00:00
abstract：:The human progesterone receptor (PR) is a ligand-dependent transcription factor and two isoforms, (PRA and PRB), can be distinguished. PROGINS, a PR polymorphic variant, affects PRA and PRB and acts as a risk-modulating factor in several gynaecological disorders. Little is known about the functional consequences of th...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.02170
更新日期：2007-02-01 00:00:00
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journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0330293
更新日期：2004-08-01 00:00:00
abstract：:The mannose 6-phosphate/IGF 2 receptor (IGF2R) is comprised of 15 extra-cellular domains that bind IGF2 and mannose 6-phosphate ligands. IGF2R transports ligands from the Golgi to the pre-lysosomal compartment and thereafter to and from the cell surface. IGF2R regulates growth, placental development, tumour suppressio...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-08-0154
更新日期：2009-04-01 00:00:00
abstract：:Corticotrophin-releasing hormone (CRH) has been identified in several peripheral tissues, including the female reproductive organs. CRH is expressed in the placenta, myometrium, epithelial endometrium and the endometrial stromal cells at all phases of the menstrual cycle. Similarly, CRH receptors are present in pregna...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0320021
更新日期：2004-02-01 00:00:00
abstract：:Human galanin (hGal) is an important neuro-modulator present in the brain, gastrointestinal system and the hypothalamo-pituitary axis. A specific receptor for hGal has been identified in various areas in human brain. A single class of high affinity binding sites was found on plasma membranes of the amygdala (Kd 0.23 n...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0130347
更新日期：1994-12-01 00:00:00
abstract：:Secretion of parathyroid hormone-related protein (PTHrP) by sheep fetal parathyroid glands is reported to be an important factor in the maintenance of a placental calcium pump. The aim of the present study was to determine whether the developing rat parathyroid glands express PTHrP or parathyroid hormone (PTH), or bot...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0170149
更新日期：1996-10-01 00:00:00
abstract：:Fatty acids are essential nutrients that contribute to several intracellular functions. Fatty acid synthesis and oxidation are known to be regulated by sterol regulatory element-binding proteins (SREBPs), which play a pivotal role in the regulation of cellular triglyceride synthesis and cholesterol biogenesis. Recent ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-17-0289
更新日期：2018-10-01 00:00:00
abstract：:The two structurally and functionally related peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) play critical roles in many physiological/pathological processes in mammals. However, the information regarding the expression and functionality of avian NMB, GRP and their receptors is limited. Here, we char...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-17-0020
更新日期：2017-07-01 00:00:00
abstract：:cAMP-response element-binding protein (CREB) is required for the induction of adipogenic transcription factors such as CCAAT/enhancer-binding proteins (C/EBPs). Interestingly, it is known from studies in other tissues that LKB1 and its substrates AMP-activated protein kinase (AMPK) and salt-inducible kinases (SIKs) ne...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-13-0296
更新日期：2014-08-01 00:00:00
