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Monolayer and suspension culture of human prolactin-secreting pituitary adenoma.

Abstract：

:In order to study the hormonal characteristics of human prolactin-secreting pituitary adenomas, in vitro monolayer and suspension cultures from human pituitary glands were established. Optimal conditions for cultures included enzymatic dispersion into viable single-cell suspensions with the use of 1% collagenase in phosphate-buffered saline solution. After pelleting the dispersed cells by centrifugation (800 rpm for 10 minutes), they were cultured in RPMI medium that contained 20% fetal calf serum and then incubated at 37degrees C in 5% CO2. Cells were subcultured weekly at a ratio of one plate to two. In an attempt to establish whether bromocriptine has a direct inhibitory effect on pituitary secretion of prolactin (PRL), variable doses of bromocriptine were added to duplicate plates. The addition of bromocriptine to the culture medium induced suppression of PRL within 7 days. In conclusion, this study demonstrated that either monolayer of suspension cultures of human PRL secreting adenomas can be established, and that bromocriptine in doses of 1 ng/plate or more has a direct inhibitory effect on the secretion of PRL.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Kletzky OA,Marrs RP,Rundall TT,Weiss MH,Beierle JW

doi

10.1016/0002-9378(80)90084-8

subject

Has Abstract

pub_date

1980-11-15 00:00:00

pages

660-4

issue

6

eissn

0002-9378

issn

1097-6868

pii

0002-9378(80)90084-8

journal_volume

138

pub_type

杂志文章

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