Abstract：
:The non-covalent interactions of benzo[a]pyrene (BP) and several of its hydroxylated metabolites with ligandin, aminoazodye-binding protein A (Z-protein, fatty acid binding protein) and lecithin bilayers have been studied by equilibrium dialysis, an adsorption technique and fluorescence spectroscopy. Binding affinities expressed as v/c (where v = moles of BP or BP metabolite bound per mole of protein or lipid and c = unbound concentration), were measured at concentrations sufficiently low that there was no self-association of the unbound compounds as judged by their fluorescence characteristics. 3-Hydroxybenzo[a]pyrene (BP-3-phenol), 4,5-dihydro-4,5-dihydroxybenzo[a]pyrene (BP-4,5-dihydrodiol) and 7,8-dihydro-7,8-dihydroxybenzo[a]pyrene (BP-7,8-dihydrodiol) bind more strongly (v/c = 10(5)-5 x 10(5) l x mol-1) to all three binders than does BP itself (v/c = 10(4)-7 x 10(4) l x mol-1). 9,10-Dihydro-9,10-dihydroxybenzo[a]pyrene (BP-9,10-dihydrodiol) binds to ligandin with an affinity similar to those of the other BP metabolites studied here, but binds much less strongly to both protein A and lecithin (v/c = 10(4) and 3 x 10(4) x mol-1, respectively). The low affinity of BP-9,10-dihydrodiol for lecithin would account for earlier findings that on incubation of BP with isolated rat hepatocytes, this metabolite egressed from the cells to the extracellular medium much more readily than either BP-4,5-dihydrodiol or BP-7,8-dihydrodiol. Calculations based on these results suggest that within hepatocytes BP and its metabolites, including BP-9,10-dihydrodiol, will be found almost exclusively associated (> 98%) with lipid membranes.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Tipping E,Moore BP,Jones CA,Cohen GM,Ketterer B,Bridges JWdoi
10.1016/0009-2797(80)90096-4subject
Has Abstractpub_date
1980-11-01 00:00:00pages
291-304issue
3eissn
0009-2797issn
1872-7786pii
0009-2797(80)90096-4journal_volume
32pub_type
杂志文章abstract：:Despite the great progress made in setting the basis for the molecular diversity of acetylcholinesterase (AChE), an explanation for the existence of two types of amphiphilic subunits, with and without glicosylphosphatidylinositol (GPI) (Types I and II), has not been provided yet. In searching whether, as for the defic...
journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/j.cbi.2008.04.018
更新日期：2008-09-25 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2006.09.013
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journal_title：Chemico-biological interactions
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abstract：:Recently, it has been shown that drimane-type sesquiterpenoids isolated from Zygogynum pancheri, a species native to New Caledonia, possessed significant α-amylase inhibitory activities. To further explore their antidiabetic potential, we investigated the effect of 1β-O-(E-cinnamoyl)-6α-hydroxy-9epi-polygodial (D) and...
journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/s0009-2797(99)00035-6
更新日期：1999-05-14 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/0009-2797(87)90024-x
更新日期：1987-01-01 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/0009-2797(76)90095-8
更新日期：1976-02-01 00:00:00
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2015.03.003
更新日期：2015-05-05 00:00:00
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2016.04.033
更新日期：2016-06-25 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/j.cbi.2010.06.013
更新日期：2010-10-06 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/0009-2797(95)03630-5
更新日期：1995-10-20 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/s0009-2797(00)00294-5
更新日期：2001-01-30 00:00:00
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journal_title：Chemico-biological interactions
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doi：10.1016/0009-2797(93)90002-g
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journal_title：Chemico-biological interactions
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doi：10.1016/0009-2797(87)90093-7
更新日期：1987-01-01 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/s0009-2797(01)00295-2
更新日期：2002-01-22 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：1976-07-01 00:00:00
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journal_title：Chemico-biological interactions
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更新日期：2020-01-25 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章,评审
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更新日期：2007-04-25 00:00:00
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2009.06.014
更新日期：2009-10-30 00:00:00
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journal_title：Chemico-biological interactions
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更新日期：2015-06-05 00:00:00
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2017.10.009
更新日期：2018-11-01 00:00:00
abstract：:In this study, we assessed the efficacy of the Reactive Skin Decontamination Lotion (RSDL®) Kit against parathion and aldicarb pesticide dermal exposure in a guinea pig model. The pesticides inhibit acetylcholinesterase (AChE) leading to signs and symptoms of hyperactivity of organs due to accumulation of acetylcholin...
journal_title：Chemico-biological interactions
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更新日期：2020-02-25 00:00:00
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2012.01.008
更新日期：2012-03-05 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/s0009-2797(99)00095-2
更新日期：1999-07-01 00:00:00
abstract：:Type II diabetes is recognized as a major risk factor for death due to cardiovascular complications such as coronary heart disease (CHD), but the complex interplay between these two diseases remains poorly understood. Suppression of oxidative stress, apoptosis, and inflammation of endothelial cells is a valuable treat...
journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/j.cbi.2019.108864
更新日期：2020-01-05 00:00:00
