Abstract：
:The present study shows the existence of both Ca2+-dependent and EDTA-resistant hydrolysing activities against HDCP and paraoxon in the particulate and soluble fractions of hen, rat and rabbit liver. HDCP was more extensively hydrolysed than paraoxon in both subcellular fractions and each of three individuals of the three animal species under study in spite of wide interindividual variations. However the ratio of HDCP versus paraoxon hydrolysing activity (HDCPase/paraoxonase), although within the same order of magnitude, cannot be considered as constant as it ranges one- to seven-fold between individuals of the same species. Also there is no constant ratio of Ca2+-dependent/EDTA-resistant activities. Rabbit liver showed the highest rates of Ca2+-dependent hydrolysis for both organophosphorus compounds whereas the hen paraoxonase activity was not inhibited by EDTA. The stereospecific hydrolysis of HDCP was mostly a Ca2+-dependent one, the S-HDCP isomer being hydrolysed faster than the R-HDCP one. The suggestion is made that HDCP could be conveniently used to measure PTE activity in the liver.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Monroy-Noyola A,Sogorb MA,Díaz-Alejo N,Níguez N,Barril J,Vicedo JL,Escudero MA,Vilanova Edoi
10.1016/s0009-2797(99)00035-6subject
Has Abstractpub_date
1999-05-14 00:00:00pages
257-62eissn
0009-2797issn
1872-7786pii
S0009-2797(99)00035-6journal_volume
119-120pub_type
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journal_title：Chemico-biological interactions
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