Combination of chrysin and cisplatin promotes the apoptosis of Hep G2 cells by up-regulating p53.


:Cisplatin is a chemotherapy drug commonly used for the treatment of human cancers, however, drug resistance poses a major challenge to clinical application of cisplatin in cancer therapy. Recent studies have shown that chrysin, a natural flavonoid widely found in various plants and foods, demonstrated effective anti-cancer activity. In the present study, we found that the combination chrysin and cisplatin significantly enhanced the apoptosis of Hep G2 cancer cells. Combination of chrysin and cisplatin increased the phosphorylation and accumulation of p53 through activating ERK1/2 in Hep G2 cells, which led to the overexpression of the pro-apoptotic proteins Bax and DR5 and the inhibition of the anti-apoptotic protein Bcl-2. In addition, combination of chrysin and cisplatin promoted both extrinsic apoptosis by activating caspase-8 and intrinsic apoptosis by increasing the release of cytochrome c and activating caspase-9 in Hep G2 cells. Our results suggest that combination of chrysin and cisplatin is a promising strategy for chemotherapy of human cancers that are resistant to cisplatin.


Chem Biol Interact


Li X,Huang JM,Wang JN,Xiong XK,Yang XF,Zou F




Has Abstract


2015-05-05 00:00:00












  • Traces of copper ions deplete glutathione in human hepatoma cell cultures with low cysteine content.

    abstract:BACKGROUND:Cell death induced by intracellular glutathione depletion has been reported to be dependent on the presence of trace amounts of extracellular copper ions. Since little is known about the relationship between glutathione depletion and copper homeostasis, we have in the present study further investigated the r...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Hultberg M,Isaksson A,Andersson A,Hultberg B

    更新日期:2007-04-05 00:00:00

  • DNA damage and repair in epithelial (mucous) cells and crypt cells from isolated colon.

    abstract::Colon epithelium is made up of two general classes of cells, surface cells which are post-mitotic and crypt cells which contain the proliferative population. Their relative vulnerability to environmental damage and ability to perform DNA repair are important issues in colon carcinogenesis. DNA damage and repair was st...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Kulkarni MS,Yielding KL

    更新日期:1985-01-01 00:00:00

  • Binding of [-14C]aflatoxin B1 to cellular macromolecules in the rat and hamster.

    abstract::The uptake and binding of ring-labelled [-14C]aflatoxin B1 (AFB1) by rat and hamster liver and kidney has been studied, the former species being extremely sensitive to the carcinogenic action of AFB, whereas the latter is resistant. In contrast to an earlier report (Lijinsky et al, Cancer Res., 30 (1970) 2280-2283, b...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Garner RC,Wright CM

    更新日期:1975-08-01 00:00:00

  • Differences in antioxidant potential of chalcones in human serum: In vitro study.

    abstract:INTRODUCTION:An imbalance between oxidants and antioxidants in favour of oxidants, potentially leading to damage, is termed oxidative stress. Antioxidants (AO), either enzymatic or non-enzymatic, are the ones that can reduce diverse effects of pro-oxidants such as DNA, proteins and lipids damage. Chalcones (1,3-diaryl-...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Janković T,Turković N,Kotur-Stevuljević J,Vujić Z,Ivković B

    更新日期:2020-06-01 00:00:00

  • Pyrazolone incorporating bipyridyl metallointercalators as effective DNA, protein and lung cancer targets: Synthesis, characterization and in vitro biocidal evaluation.

    abstract::Pyrazolone based metal complexes have strong bio-activity but the anti-cancer mechanism of these derivatives is not fully understood. In recent years, Cu(II) complexes have attracted the interest of researchers increasingly because of their high antitumor activities that are usually related to DNA binding. The reactio...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Vyas KM,Devkar RV,Prajapati A,Jadeja RN

    更新日期:2015-10-05 00:00:00

  • Hemoglobin binding of monocyclic aromatic amines: molecular dosimetry and quantitative structure activity relationships for the N-oxidation.

    abstract::Aromatic amines are important intermediates in industrial manufacturing. N-oxidation to the N-hydroxyarylamines is a key step determining the genotoxic properties of aromatic amines. N-hydroxyarylamines can form adducts with DNA, with tissue proteins and with the blood proteins albumin and hemoglobin in a dose-depende...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Sabbioni G

    更新日期:1992-01-01 00:00:00

  • Modulation of mitochondrial respiration rate and calcium-induced swelling by new cromakalim analogues.

    abstract::A cellular model of cardiomyocytes (H9c2 cell line) and mitochondria isolated from mouse liver were used to understand the drug action of BPDZ490 and BPDZ711, two benzopyran analogues of the reference potassium channel opener cromakalim, on mitochondrial respiratory parameters and swelling, by comparing their effects ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Mouithys-Mickalad A,Ceusters J,Charif M,El Moualij B,Schoumacher M,Plyte S,Franck T,Bettendorff L,Pirotte B,Serteyn D,de Tullio P

    更新日期:2020-11-01 00:00:00

  • Studies on rat hepatic hydroxysteroid sulfotransferase--immunochemistry, development and pI variants.

    abstract::Rat hepatic hydroxysteroid sulfotransferase with sulfoconjugates androsterone (androsterone-sulfating sulfotransferase) is an oligomer consisting of several subunits with distinct pI values but with the same molecular mass (pI variants). N-terminal amino acid sequences of the pI variants are all identical. The enzyme ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Homma H,Nakagome I,Kamakura M,Hirota M,Takahashi M,Matsui M

    更新日期:1994-06-01 00:00:00

  • Peroxisome proliferation and associated effects caused by perfluorooctanoic acid in vitamin A-deficient mice.

    abstract::Vitamin A-deficient male mice were treated for 10 days with 0.02% perfluorooctanoic acid (PFOA) in their diet. The effects of this highly potent peroxisome proliferator on peroxisomal palmitoyl-CoA oxidation and lauroyl-CoA oxidase activities were reduced by 3.1-7.5 times in comparison to the values obtained with mice...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Sohlenius AK,Andersson K,Olsson J,DePierre JW

    更新日期:1995-10-20 00:00:00

  • Cytochrome P450 expression and metabolic activation of cooked food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in MCF10A breast epithelial cells.

    abstract::The cytochrome P450 expression profile was determined in the MCF10A human breast epithelial cell line, as was the ability of this cell line to catalyze the bioactivation of the cooked food mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Using non-quantitative reverse transcription-polymerase chain rea...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Thomas RD,Green MR,Wilson C,Weckle AL,Duanmu Z,Kocarek TA,Runge-Morris M

    更新日期:2006-04-15 00:00:00

  • Comparative effect of a family of substituted thiopseudoureas on protein synthesis by rat liver and Walker carcinoma ribosomes.

    abstract::In order to assess the role played respectively by the pseudothiourea group and the alkylic chain in the inhibition of protein synthesis and tumour growth caused by compound AHR-1911, a series of eight related substances were studied. The blockade of protein synthesis on liver and Walker carcinoma ribosomes and on sus...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Carmona A,Gonzalez-Cadavid NF

    更新日期:1978-09-01 00:00:00

  • Single immunoglobulin IL-1 receptor-related protein attenuates the lipopolysaccharide-induced inflammatory response in A549 cells.

    abstract::The lipopolysaccharide (LPS)-Toll-like receptor 4 (TLR4) signaling pathway in alveolar epithelial cells plays an important role in many pathologic processes such as acute lung injury (ALI). The single immunoglobulin IL-1 receptor-related protein (SIGIRR) is an inhibitor of LPS-TLR4 signaling, but its expression and fu...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Feng T,Yunfeng N,Jinbo Z,Zhipei Z,Huizhong Z,Li L,Tao J,Yunjie W

    更新日期:2010-02-12 00:00:00

  • Expression of cholinesterases in brain and non-brain tumours.

    abstract::Although the involvement of cholinesterases (ChEs) in the removal of acetylcholine (ACh) at cholinergic synapses is firmly established, there is evidence to suggest that acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) take part in several cellular processes. The early expression of ChE genes during embry...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Vidal CJ

    更新日期:2005-12-15 00:00:00

  • Biopolymer metal catalysts for the hydrolysis of nerve gases.

    abstract::Glucosamine oligomers--monomer through tetramer--form complexes with Cu2+ that catalyse the hydrolysis of the 'nerve gas' 1,2,2-trimethylpropyl- methylphosphonofluroidate (soman) by cleaving the P-F bond. A 1/1 glucosamine/Cu2+ ratio whether as glucosamine or glucosamine units, gives the highest hydrolytic rate over t...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Hoskin FC,Rajan KS,Remy D,Walker JE

    更新日期:1993-06-01 00:00:00

  • In vivo repair of rat liver DNA damaged by dimethylnitrosamine or diethylnitrosamine.

    abstract::Effects of hepatocarcinogens dimethylnitrosamine (DMN) and diethylnitrosamine (DEN) on the sedimentation pattern of rat liver DNA in alkaline sucrose gradients were studied with regard to time and dose dependency. Both DMN (10 mg/kg body weight) and den (13.4 or 134 mg/kg) induced appreciably decreased DNA sedimentati...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Den Engelse L,Philippus EJ

    更新日期:1977-10-01 00:00:00

  • Human erythrocytes and molecular models of cell membranes are affected in vitro by Balbisia peduncularis (Amancay) extracts.

    abstract::Balbisia peduncularis, also known as "Amancay", is a plant of the Ledocarpaceae family that can be found in the Atacama Desert in northern Chile. Infusions of the plant have long being used in traditional herbal medicine. Its chemical composition indicates the presence of flavonoids, which have antioxidant properties....

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Suwalsky M,Oyarce K,Avello M,Villena F,Sotomayor CP

    更新日期:2009-05-15 00:00:00

  • 4-Methylesculetin, a natural coumarin with intestinal anti-inflammatory activity, elicits a glutathione antioxidant response by different mechanisms.

    abstract::4-methylesculetin (4 ME) is a natural antioxidant coumarin with protective effects on the intestinal inflammation, in which oxidative stress plays a key role in its aetiology and pathophysiology. Based on this, we examined the antioxidant molecular mechanisms involved in the intestinal anti-inflammatory activity of th...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Tanimoto A,Witaicenis A,Caruso ÍP,Piva HMR,Araujo GC,Moraes FR,Fossey MC,Cornélio ML,Souza FP,Di Stasi LC

    更新日期:2020-01-05 00:00:00

  • Effect of several analogs of 2,4,6-triphenyldioxane-1,3 on constitutive androstane receptor activation.

    abstract::2,4,6-Triphenyldioxane-1,3 (TPD) is a highly effective species-specific inducer of CYP2В in rats. Several analogs of TPD were synthesized to verify a hypothesis that minor changes in the inducer structure can cause changes in induction abilities (R=H, cisTPD and transTPD; R=N(CH(3))(2), transpDMA; R=NO(2), transpNO(2)...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Pustylnyak V,Yarushkin A,Kachaylo E,Slynko N,Lyakhovich V,Gulyaeva L

    更新日期:2011-07-15 00:00:00

  • Benzalkonium chloride and cetylpyridinium chloride induce apoptosis in human lung epithelial cells and alter surface activity of pulmonary surfactant monolayers.

    abstract::Quaternary ammonium compounds (e.g., benzalkonium chloride (BAC) and cetylpyridinium chloride (CPC)) constitute a group of cationic surfactants are widely used for personal hygiene and medical care despite the potential pulmonary toxicity. To examine whether BAC and CPC aerosols deposited in the alveolar region alter ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Kanno S,Hirano S,Kato H,Fukuta M,Mukai T,Aoki Y

    更新日期:2020-02-01 00:00:00

  • An essential oil and its major constituent isointermedeol induce apoptosis by increased expression of mitochondrial cytochrome c and apical death receptors in human leukaemia HL-60 cells.

    abstract::An essential oil from a lemon grass variety of Cymbopogon flexuosus (CFO) and its major chemical constituent sesquiterpene isointermedeol (ISO) were investigated for their ability to induce apoptosis in human leukaemia HL-60 cells because dysregulation of apoptosis is the hallmark of cancer cells. CFO and ISO inhibite...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Kumar A,Malik F,Bhushan S,Sethi VK,Shahi AK,Kaur J,Taneja SC,Qazi GN,Singh J

    更新日期:2008-02-15 00:00:00

  • Effect of drug metabolism inducer and inhibitor on O,O,S-trimethyl phosphorothioate-induced delayed toxicity in rats.

    abstract::Oral administration of O,O,S-trimethyl phosphorothioate (OOS), an impurity present in widely used organophosphorus insecticides, causes delayed toxicity in rats, i.e., death occurring as late as 28 days after the treatment. The signs of toxicity include body weight loss (maximum on day 3), red staining around the nose...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Imamura T,Hasegawa L,Gandy J,Fukuto TR

    更新日期:1983-07-01 00:00:00

  • 2β, 3β, 23-trihydroxy-urs-12-ene-28-olic acid (TUA) isolated from Actinidia chinensis Radix inhibits NCI-H460 cell proliferation by decreasing NF-κB expression.

    abstract::A natural ursolic compound, 2β, 3β, 23-trihydroxy-urs-12-ene-28-olic acid (TUA) was isolated from the root of Actinidia chinensis Planch (A. chinensis Radix). Since a large number of triterpenoid compound has marked anticancer effects toward various types of cancer cell lines in vitro, this study was carried out to in...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Cheng QL,Li HL,Huang ZQ,Chen YJ,Liu TS

    更新日期:2015-10-05 00:00:00

  • Rutin alleviates hypoxia/reoxygenation-induced injury in myocardial cells by up-regulating SIRT1 expression.

    abstract::Rutin possesses multiple pharmacological activities, including the cardioprotective effect. The present study aimed to evaluate the protective effects of rutin on hypoxia/reoxygenation (H/R)-induced myocardial injury and its underlying mechanism involved. H9c2 cells were pretreated with 50 μM rutin or combined with 1 ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Yang H,Wang C,Zhang L,Lv J,Ni H

    更新日期:2019-01-05 00:00:00

  • Bis(12)-hupyridone, a novel acetylcholinesterase inhibitor, protects against glutamate-induced neuronal excitotoxicity via activating α7 nicotinic acetylcholine receptor/phosphoinositide 3-kinase/Akt cascade.

    abstract::Bis(12)-hupyridone (B12H), derived from the Chinese medicinal component huperzine A, was originally designed as a novel acetylcholinesterase (AChE) inhibitor. In this paper, we report that B12H (24-h pretreatment) effectively blocked glutamate-induced neuronal excitotoxicity in cerebellar granule neurons (CGNs). Howev...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Cui W,Hu S,Chan HH,Luo J,Li W,Mak S,Choi TC,Rong J,Carlier PR,Han Y

    更新日期:2013-03-25 00:00:00

  • The release of lysosomal arylsulfatase from liver lysosomes exposed to 2-chloroethylethyl sulfide.

    abstract::Treatment of a lysosome-rich fraction from liver with 2-chloroethylethyl sulfide resulted in a dose-dependent release of arylsulfatase. The inclusion of Ca2+ enhanced the enzyme release by approximately 2.3-fold. The enhancing effect of Ca2+, showing an EC50 value of 30 mM, was mimicked by neither Mg2+ nor Mn2+. Studi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Shin S,Choi DS,Kim YB,Cha SH,Sok DE

    更新日期:1995-08-18 00:00:00

  • Protective effect of a novel cystine C(60) derivative on hydrogen peroxide-induced apoptosis in rat pheochromocytoma PC12 cells.

    abstract::Oxidative stress has been considered as a major cause of cellular injuries in a variety of clinical abnormalities, especially neural diseases. One of the effective ways to prevent the reactive oxygen species (ROS) mediated cellular injury is dietary or pharmaceutical augmentation of free radical scavengers. In the pre...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Hu Z,Guan W,Wang W,Huang L,Xing H,Zhu Z

    更新日期:2007-04-25 00:00:00

  • HTLV-1 Tax: Linking transformation, DNA damage and apoptotic T-cell death.

    abstract::The human T-cell leukemia virus type I (HTLV-1) is the causative agent of adult T-cell leukemia (ATL), an aggressive CD4-positive T-cell neoplasia. The HTLV-1 proto-oncogene Tax, a potent transcriptional activator of cellular and viral genes, is thought to play a pivotal role in the transforming properties of the viru...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审


    authors: Chlichlia K,Khazaie K

    更新日期:2010-11-05 00:00:00

  • Methylseleninic acid and sodium selenite induce severe ER stress and subsequent apoptosis through UPR activation in PEL cells.

    abstract::Selenium compounds such as methylseleninic acid (MSA) and sodium selenite (SS) have been widely evaluated as potential anti-cancer agents in the clinical setting. Primary effusion lymphoma (PEL) is a non-Hodgkin's B-cell lymphoma, associated with immunosuppressed individuals, such as post-transplant or AIDS patients. ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Shigemi Z,Manabe K,Hara N,Baba Y,Hosokawa K,Kagawa H,Watanabe T,Fujimuro M

    更新日期:2017-03-25 00:00:00

  • Reaction of colloidal silica with membranes of intact mammalian cells.

    abstract::Colloidal silical particles were produced at a size that permitted reaction with human erythrocytes and rat macrophages without affecting cell integrity. Binding of colloid was shown by increased electrophoretic mobility of red cells and also resulted in changes in the surface topography of red cells as seen with scan...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Cantrell AC,Ellis P

    更新日期:1983-04-01 00:00:00

  • In vitro acylation of the xi-amino group of L-lysine in calf thymus histones by the carcinogen, beta-propiolactone.

    abstract::We had previously reported that the carcinogen, beta-propiolactone (BPL) reacted in vitro with histones in whole mouse skin chromatin and that among the histone classes BPL was preferentially bound to the lysine-rich histones H1 and H1 degrees. In order to determine if in vitro reaction of BPL with calf thymus histone...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Segal A,Garte SJ

    更新日期:1976-12-01 00:00:00