Combination of chrysin and cisplatin promotes the apoptosis of Hep G2 cells by up-regulating p53.


:Cisplatin is a chemotherapy drug commonly used for the treatment of human cancers, however, drug resistance poses a major challenge to clinical application of cisplatin in cancer therapy. Recent studies have shown that chrysin, a natural flavonoid widely found in various plants and foods, demonstrated effective anti-cancer activity. In the present study, we found that the combination chrysin and cisplatin significantly enhanced the apoptosis of Hep G2 cancer cells. Combination of chrysin and cisplatin increased the phosphorylation and accumulation of p53 through activating ERK1/2 in Hep G2 cells, which led to the overexpression of the pro-apoptotic proteins Bax and DR5 and the inhibition of the anti-apoptotic protein Bcl-2. In addition, combination of chrysin and cisplatin promoted both extrinsic apoptosis by activating caspase-8 and intrinsic apoptosis by increasing the release of cytochrome c and activating caspase-9 in Hep G2 cells. Our results suggest that combination of chrysin and cisplatin is a promising strategy for chemotherapy of human cancers that are resistant to cisplatin.


Chem Biol Interact


Li X,Huang JM,Wang JN,Xiong XK,Yang XF,Zou F




Has Abstract


2015-05-05 00:00:00












  • Roles of glucuronidation and UDP-glucuronosyltransferases in xenobiotic bioactivation reactions.

    abstract::Glucuronide conjugates represent one of the major types of naturally occurring phase 2 metabolites of xenobiotics and endobiotics. The process underlying their formation, glucuronidation, is normally considered detoxifying, because glucuronides usually possess less intrinsic biological or chemical activity than their ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审


    authors: Ritter JK

    更新日期:2000-12-01 00:00:00

  • Effects of hypoxia and ethanol on xanthine oxidase of isolated rat hepatocytes: conversion from D to O form and leakage from cells.

    abstract::The combined effects of ethanol and hypoxia on the conversion of xanthine dehydrogenase (D form) to xanthine oxidase (O form) and on the leakage of the enzyme from isolated rat hepatocytes was studied. Time-dependent death of cells occurred during incubation in hypoxic conditions. Ethanol (40 mM) had only a moderate e...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Battelli MG,Abbondanza A,Stirpe F

    更新日期:1992-06-15 00:00:00

  • Differentiation of human placental BeWo cells by the environmental contaminant benzo(a)pyrene.

    abstract::Polycyclic aromatic hydrocarbons (PAHs) such as benzo(a)pyrene (BaP) are widely-distributed environmental contaminants known to exert toxic effects in various tissues, including placenta. PAHs have notably been shown to inhibit proliferation of trophoblastic cells. The present study was designed to determine whether P...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Le Vee M,Kolasa E,Jouan E,Collet N,Fardel O

    更新日期:2014-03-05 00:00:00

  • DNA damage and repair in epithelial (mucous) cells and crypt cells from isolated colon.

    abstract::Colon epithelium is made up of two general classes of cells, surface cells which are post-mitotic and crypt cells which contain the proliferative population. Their relative vulnerability to environmental damage and ability to perform DNA repair are important issues in colon carcinogenesis. DNA damage and repair was st...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Kulkarni MS,Yielding KL

    更新日期:1985-01-01 00:00:00

  • Osthole induces cell cycle arrest and apoptosis in head and neck squamous cell carcinoma by suppressing the PI3K/AKT signaling pathway.

    abstract:BACKGROUND:Head and neck squamous cell carcinoma (HNSCC) is one of the most common lethal tumors with a high recurrence rate and low survival rate. Therefore, an urgent need exists for novel and effective treatment strategies for HNSCC patients. METHODS:Osthole, a natural ingredient extracted from Cnidium monnieri (L....

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Yang J,Zhu XJ,Jin MZ,Cao ZW,Ren YY,Gu ZW

    更新日期:2020-01-25 00:00:00

  • Inhibition of the conversion of pre-interleukins-1 alpha and 1 beta to mature cytokines by p-benzoquinone, a metabolite of benzene.

    abstract::Chronic exposure of humans to benzene causes severe bone marrow cell depression leading to aplastic anemia. Marrow stromal macrophage dysfunction and deficient interleukin-1 production has been reported for patients with severe aplastic anemia. The stromal macrophage, a target of benzene toxicity, is involved in hemat...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Niculescu R,Bradford HN,Colman RW,Kalf GF

    更新日期:1995-12-22 00:00:00

  • Covalent binding of benzene and its metabolites to DNA in rabbit bone marrow mitochondria in vitro.

    abstract::Rabbit bone marrow mitochondria, stripped of their outer membrane (mitoplasts), have been shown to carry out the NADPH-dependent bioactivation of radiolabelled benzene in vitro to metabolites capable of covalently binding to mtDNA, thereby inhibiting transcription. The metabolites of benzene produced in bone marrow ce...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Rushmore T,Snyder R,Kalf G

    更新日期:1984-04-01 00:00:00

  • Action of corilagin on hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic rats.

    abstract::Diabetes mellitus is the world's most common endocrine disease involving metabolic disorders of carbohydrate, protein and fat. This study was undertaken to investigate the anti-diabetic activity of corilagin, a member of polyphenolic tannins used against hyperglycemia and many other diseases in well-known animal model...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Nandini HS,Naik PR

    更新日期:2019-02-01 00:00:00

  • Anti-inflammatory effects of dabrafenib on polyphosphate-mediated vascular disruption.

    abstract::The screening of bioactive compound libraries can be an effective approach for repositioning FDA-approved drugs or discovering new treatments for human diseases. Previous studies have reported polyphosphate (PolyP)-mediated vascular inflammatory responses such as disruption of vascular integrity. Dabrafenib is a B-Raf...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Lee S,Ku SK,Bae JS

    更新日期:2016-08-25 00:00:00

  • Effect of glucocorticoid-induced oxidative stress on the expression of Cbfa1.

    abstract::Glucocorticoids therapy is strongly limited since extended glucocorticoids can cause serious side effects, including increased susceptibility to develop the bone disease osteoporosis. Despite its side effects recognized importance to clinicians, seldom is known about how glucocorticoids directly impact bone-forming os...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Feng YL,Tang XL

    更新日期:2014-01-25 00:00:00

  • Drosophila lacking a homologue of mammalian ALDH2 have multiple fitness defects.

    abstract::Little is known about the roles of aldehyde dehydrogenases in non-vertebrate animals. We recently showed that in Drosophila melanogaster, an enzyme with ∼70% amino acid identity to mammalian ALDH2 is necessary for detoxification of dietary ethanol. To investigate other functions of this enzyme, DmALDH, encoded by the ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Chakraborty M,Fry JD

    更新日期:2011-05-30 00:00:00

  • In vitro cytotoxicity of montelukast in HAPI and SH-SY5Y cells.

    abstract::Montelukast is a cysteinyl leukotriene (CysLT) receptor antagonist with efficacy against a variety of diseases, including asthma and inflammation-related conditions. However, various neuropsychiatric events (NEs) suspected to be related to montelukast have been reported recently, with limited understanding on their as...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Tseng YT,Cox TM,Grant GD,Arora D,Hall S,McFarland AJ,Ekberg J,Rudrawar S,Anoopkumar-Dukie S

    更新日期:2020-08-01 00:00:00

  • Studies on the metabolic activation of beta-keto nitrosamines: mechanisms of DNA methylation by N-(2-oxopropyl)-N-nitrosourea and N-nitroso-N-acetoxymethyl-N-2-oxopropylamine.

    abstract::At pH 7.35, N-(2-oxopropyl)-N-nitrosourea (OPNU) reacted with calf thymus DNA to yield O6-methylguanine, 7-methylguanine and 3-methyladenine. Kinetic measurements of the base catalyzed decomposition of OPNU and the extent of methylation of DNA by OPNU suggested that methylnitrosourea is not formed as an intermediate p...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Leung KH,Archer MC

    更新日期:1984-02-01 00:00:00

  • 2β, 3β, 23-trihydroxy-urs-12-ene-28-olic acid (TUA) isolated from Actinidia chinensis Radix inhibits NCI-H460 cell proliferation by decreasing NF-κB expression.

    abstract::A natural ursolic compound, 2β, 3β, 23-trihydroxy-urs-12-ene-28-olic acid (TUA) was isolated from the root of Actinidia chinensis Planch (A. chinensis Radix). Since a large number of triterpenoid compound has marked anticancer effects toward various types of cancer cell lines in vitro, this study was carried out to in...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Cheng QL,Li HL,Huang ZQ,Chen YJ,Liu TS

    更新日期:2015-10-05 00:00:00

  • DNA damage, prophage induction and mutation by furazolidone.

    abstract::Ultraviolet absorption data and thermal chromatography through hydroxyapatite (HAP) column revealed that furazolidone treatment of Vibrio cholerae cells produced more than 80% of DNA reversibly bihelical due to the formation of interstrand cross-links and the reaction obeyed a first order relation. Sensitivities of th...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Chatterjee SN,Banerjee SK,Pal AK,Basak J

    更新日期:1983-08-01 00:00:00

  • Identification and characterization of functional antioxidant response elements in the promoter of the aldo-keto reductase AKR1B10 gene.

    abstract::AKR1B10 is a human-type aldo-keto reductase. The up-regulation of AKR1B10 has been associated with various cancers including non-small cell lung carcinoma, viral and bacterial infections, and skin diseases. However, the mechanisms underlying AKR1B10 gene regulation are not fully understood. We previously indicated the...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Nishinaka T,Miura T,Shimizu K,Terada T

    更新日期:2017-10-01 00:00:00

  • CRKL promotes cell proliferation in gastric cancer and is negatively regulated by miR-126.

    abstract::V-crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) is a member of CRK family and act as an adaptor protein participating in intra-cellular signal transduction. The role of CRKL in gastric cancer (GC) remains unclear. In this study, we show that CRKL was aberrantly highly expressed in both GC tumor specimens a...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Wang J,Chen X,Li P,Su L,Yu B,Cai Q,Li J,Yu Y,Liu B,Zhu Z

    更新日期:2013-11-25 00:00:00

  • The AChE membrane-binding tail PRiMA is down-regulated in muscle and nerve of mice with muscular dystrophy by merosin deficiency.

    abstract::Since Duchenne muscular dystrophy was attributed to mutations in the dystrophin gene, more than 30 genes have been found to be causally related with muscular dystrophies, about half of them encoding proteins of the dystrophin-glycoprotein complex (DGC). Through laminin-2, the DGC bridges the muscle cytoskeleton and th...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Vidal CJ,Montenegro MF,Muñoz-Delgado E,Campoy FJ,Cabezas-Herrera J,Moral-Naranjo MT

    更新日期:2013-03-25 00:00:00

  • Association of variants of the -116 site of the butyrylcholinesterase BCHE gene to enzyme activity and body mass index.

    abstract::Butyrylcholinesterase (BChE) is coded by the BCHE gene that presents four exons. The non-codifying exon 1 presents two variants -116G and -116A, being -116A preferentially in cis conformation with the 539T variant (K) of exon 4 which was associated with lower BChE activity and lower body mass index (BMI) variance. Thi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Furtado-Alle L,Andrade FA,Nunes K,Mikami LR,Souza RL,Chautard-Freire-Maia EA

    更新日期:2008-09-25 00:00:00

  • Zinc oxide nanoparticles inhibit dimethylnitrosamine induced liver injury in rat.

    abstract::Dimethylnitrosamine (DMN) is a potent hepatotoxic, carcinogenic and mutagenic compound. It induces massive liver cell necrosis and death in experimental animals. Several drugs have been tested in the past for their protective behavior against DMN toxicity. However, it is for the first time that therapeutic interventio...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Rani V,Verma Y,Rana K,Rana SVS

    更新日期:2018-11-01 00:00:00

  • Formation of DNA adducts in human buccal epithelial cells exposed to acetaldehyde and methylglyoxal in vitro.

    abstract::Acetaldehyde (AA) and methylglyoxal (MG) are reactive, ubiquitous aldehydes, present in the environment and endogenously formed in animals and humans. They have both been shown to readily form DNA adducts under simulated physiological conditions. We report here on the use of cultured normal and SV40T antigen-immortali...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Vaca CE,Nilsson JA,Fang JL,Grafström RC

    更新日期:1998-01-30 00:00:00

  • Characterization of paraoxonase activity in pericardial fluid: usefulness as a marker of coronary disease.

    abstract::In this study, the presence of paraoxonase activity in pericardial fluid was demonstrated. A comparison of some properties, such as optimum pH, stability versus pH, heat inactivation, effect of inhibitors, isoelectric point and kinetic parameters (Km and Vmax), between plasma and pericardial fluid paraoxonase was made...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Hernández AF,Pla A,Valenzuela A,Gil F,Villanueva E

    更新日期:1993-06-01 00:00:00

  • Uremic toxin indoxyl sulfate suppresses myocardial Cx43 assembly and expression via JNK activation.

    abstract::Heart rhythm disturbances have been widely recognized as major triggers of cardiovascular (CV) mortality in chronic kidney disease (CKD) patients. Connexin 43 (Cx43)-composed gap junctions are essential in cardiomyocyte synchronization and may be involved in the pathological response to uremic toxins. Indoxyl sulfate ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Changchien CY,Sung MH,Chang HH,Tsai WC,Peng YS,Chen Y

    更新日期:2020-03-01 00:00:00

  • The interactions of 9,10-phenanthrenequinone with glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a potential site for toxic actions.

    abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate to 1,3-diphosphoglycerate, one of the precursors for glycolytic ATP biosynthesis. The enzyme contains an active site cysteine thiolate, which is critical for its catalytic function. As part of a conti...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Rodriguez CE,Fukuto JM,Taguchi K,Froines J,Cho AK

    更新日期:2005-06-30 00:00:00

  • A peptide derived from acetylcholinesterase is a pivotal signalling molecule in neurodegeneration.

    abstract::It is now widely accepted that acetylcholinesterase (AChE) also displays non-cholinergic functions, completely independent of cholinergic transmission. Indeed, AChE has been implicated in a variety of trophic and toxic actions in a range of different systems. However, it is still uncertain what part of the AChE molecu...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Greenfield S

    更新日期:2005-12-15 00:00:00

  • Gender-specific metabolism of benz[a]anthracene in hepatic microsomes from Long-Evans and Hooded Lister rats.

    abstract::The cytochrome P450 isoforms responsible for the regio-selective metabolism of benz[a]anthracene (BA) are poorly defined but as with other polycyclic aromatic hydrocarbons (PAHs) may include members of the CYP2C sub-family. Since the expression of some of these is regulated in a gender-specific manner and may be alter...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Boyle SP,Craft JA

    更新日期:2000-03-15 00:00:00

  • 2,3,7,8-tetrachlorodibenzo-p-dioxin induces lecithin: retinol acyltransferase transcription in the rat kidney.

    abstract::Vitamin A (retinoids) has an essential role in development and throughout life of humans and animals. Consequently, effects of the environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on retinoid metabolism may be contributory to its toxicity. This study was performed to clarify the mechanism behind diox...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Hoegberg P,Schmidt CK,Nau H,Ross AC,Zolfaghari R,Fletcher N,Trossvik C,Nilsson CB,Håkansson H

    更新日期:2003-03-06 00:00:00

  • Inhibitory effect of hemin on mutagenicity of the electrophilic sulfuric acid ester of 6-hydroxymethylbenzo[a]pyrene.

    abstract::In the present study, we examined the effects of hemin on the mutagenicity of 6-sulfooxymethylbenzo[a]pyrene (SMBP) in Salmonella typhimurium TA98 and Chinese hamster lung fibroblast (V79) cells. The compound was tested for the possible chemoprotective activity against mutagenesis induced by SMBP and its precursor, 6-...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Hong ST,Cho YS,Surh YJ,Chung AS

    更新日期:1998-02-20 00:00:00

  • Peroxidase-mediated irreversible binding of arylamine carcinogens to DNA in intact polymorphonuclear leukocytes activated by a tumor promoter.

    abstract::Addition of the tumor promoter phorbol myristate acetate to polymorphonuclear leukocytes results in the oxidation of the arylamine carcinogens; [14C]benzidine, N-[14C]methylaminoazobenzene and [14C]aminofluorene to reactive intermediate(s) that bind irreversibly to the leukocyte DNA. The binding was dependent on oxyge...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Tsuruta Y,Subrahmanyam VV,Marshall W,O'Brien PJ

    更新日期:1985-02-01 00:00:00

  • Vitamin D nanoemulsion enhances hepatoprotective effect of conventional vitamin D in rats fed with a high-fat diet.

    abstract:BACKGROUND:Non-alcoholic fatty liver disease (NAFLD) is associated with hyperlipidemia, obesity and type II diabetes. Due to increasing prevalence of these diseases globally, NAFLD is considered as a common form of chronic liver diseases. Vitamin D is a fat soluble vitamin with reported anti-inflammatory, anti-oxidant ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: El-Sherbiny M,Eldosoky M,El-Shafey M,Othman G,Elkattawy HA,Bedir T,Elsherbiny NM

    更新日期:2018-05-25 00:00:00