Abstract:
:A new isolation procedure has resulted in an improved yield of stable 68S chloroplast ribosomes from Euglena gracilis var. bacillaris. Chloroplasts are isolated by suspending the cells in buffer I (sorbitol, 250 mm; sucrose, 250 mm; Ficoll, 2.5% [w/v]; magnesium acetate, 1 mm; bovine serum albumin, 0.01% [w/v]; mercaptoethanol, 14 mm; N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid, pH 7.6, 5 mm) and passing through a French press at less than 1500 pounds per square inch. The crude chloroplasts are purified by three washings with buffer II (sorbitol, 150 mm; sucrose, 150 mm; Ficoll, 2.5% [w/v]; magnesium acetate, 1 mm; bovine serum albumin, 0.01% [w/v]; mercaptoethanol, 14 mm; N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid, pH 7.6, 5 mm). Stable 68S chloroplast ribosomes are obtained when the isolated chloroplasts are resuspended in ribosome buffer (tris-HCI, pH 7.6, 10 mm; magnesium acetate, 12 mm; KCI, 60 mm) containing spermidine, 0.5 mm; mercaptoethanol, 14 mm; sucrose, 8% (w/w), passed through a French press at 4000 pounds per square inch and extracted with either 0.1% (w/v) sodium deoxycholate or 1.0% (v/v) Triton X-100. At 0 to 4 C in ribosome buffer, the purified 68S chloroplast monosome forms a 53S particle while the 35S particle, an expected product of monosome dissociation, cannot be detected. Spermidine and mercaptoethanol prevent the formation of 53S particles from 68S monosomes. The purified 53S particles derived from 68S monosomes contain 23S RNA as well as a significant amount of 16S RNA, suggesting that this particle may not be a true ribosomal subunit.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Schwartzbach SD,Freyssinet G,Schiff JAdoi
10.1104/pp.53.4.533subject
Has Abstractpub_date
1974-04-01 00:00:00pages
533-42issue
4eissn
0032-0889issn
1532-2548journal_volume
53pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::A depolarization-activated K(+) channel capable of carrying the large K(+) currents that flow from shrinking cells during movements of Samanea saman leaflets has been described in the plasmalemma of Samanea motor cell protoplasts (N Moran et al [1988] Plant Physiol 88:643-648). We now characterize this channel in grea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.424
更新日期:1990-10-01 00:00:00
abstract::The development of photochemical activity in relation to pigment and membrane protein accumulation in chloroplasts of greening wild-type barley (Hordeum vulgare L. cv. Gateway) and its virescens mutant were studied. The rate of chlorophyll accumulation per plastid was faster in the wild-type than in the mutant seedlin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.6.1392
更新日期:1982-06-01 00:00:00
abstract::With the aim to specifically study the molecular mechanisms behind photoinhibition of photosystem I, stacked spinach (Spinacia oleracea) thylakoids were irradiated at 4 degrees C with far-red light (>715 nm) exciting photosystem I, but not photosystem II. Selective excitation of photosystem I by far-red light for 130 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.4.2007
更新日期:2001-04-01 00:00:00
abstract::The O(2) concentration in intact and osmotically disrupted isolated spinach (Spinacia oleracea, L.) chloroplasts during photosynthesis was estimated. The chloroplasts were allowed to reduce 3-phosphoglycerate, CO(2), or ferricyanide in light until the rate of O(2) production was linear. When the light was turned off O...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.903
更新日期:1977-12-01 00:00:00
abstract::Using photoacoustic spectroscopy, state 1-state 2 transitions were demonstrated in vivo in intact sugar maple leaves (Acer saccharum Marsh.) by following the changes in energy storage of photosystems (PS) I and II. Energy storage measured with 650 nm modulated light (light 2) in the presence of background white light ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.1.330
更新日期:1991-09-01 00:00:00
abstract::Storage protein synthesis is dependent on available nitrogen in the seed, which may be controlled by amino acid import via specific transporters. To analyze their rate-limiting role for seed protein synthesis, a Vicia faba amino acid permease, VfAAP1, has been ectopically expressed in pea (Pisum sativum) and Vicia nar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056523
更新日期:2005-04-01 00:00:00
abstract::In conifer stems, formation of chemical defenses against insects or pathogens involves specialized anatomical structures of the phloem and xylem. Oleoresin terpenoids are formed in resin duct epithelial cells and phenolics accumulate in polyphenolic parenchyma cells. Ethylene signaling has been implicated in the induc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.089425
更新日期:2007-01-01 00:00:00
abstract::Despite ongoing research on carotenoid biosynthesis in model organisms, there is a paucity of information on pathway regulation operating in the grasses (Poaceae), which include plants of world-wide agronomic importance. As a result, efforts to either breed for or metabolically engineer improvements in carotenoid cont...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.039818
更新日期:2004-07-01 00:00:00
abstract::At maturity, the fruit of two early maturing orange cultivars, Hamlin and Pineapple (Citrus sinensis [L.] Osbeck), contained more ethylene and abscisic acid than the late maturing Valencia and Lamb Summer (C. sinensis [L.] Osbeck) cultivars. Ethylene (up to 95 nl/l in internal atmosphere) and abscisic (50 mug/kg dry w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.6.765
更新日期:1975-12-01 00:00:00
abstract::We examined the consistency of the native diploid Nicotiana attenuata (Na)'s herbivore-induced transcriptional changes in the two allotetraploid natives, Nicotiana clevelandii (Nc) and Nicotiana quadrivalvis (Nq), which are thought to be derived from hybridizations with an ancestral Na. An analysis of nuclear-encoded ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.037036
更新日期:2004-05-01 00:00:00
abstract::SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR and SCR genes results in disorganization of the quiescent center and loss of stem cell activity, resulting in the cessation of root gro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.158857
更新日期:2010-11-01 00:00:00
abstract::Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also for micronutrient delivery to humans. To investigate metal transport processes and their interactions in seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), a tonoplast transporter of the manganese (Mn) subclade of cat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01646
更新日期:2017-07-01 00:00:00
abstract::Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082743
更新日期:2006-07-01 00:00:00
abstract::Fructose-2,6-bisphosphate (Fru-2,6-bisP) was evaluated as a potential marker for the dormancy-breaking phase or the germination phase before pericarp splitting in red rice (Oryza sativa). During 4 h of imbibition at 30[deg]C, Fru-2,6-bisP of dehulled dormant and nondormant seeds increased to 0.26 and 0.38 pmol embryo-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.4.1365
更新日期:1995-04-01 00:00:00
abstract::Bipyridinium herbicides, including paraquat and diquat, are believed to act by generating highly reactive, oxygen-centered free radicals within chloroplasts when treated plants are exposed to sunlight. This hypothesis has not yet been confirmed by direct chemical measurements of specific free radicals. We studied para...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.4.1267
更新日期:1989-08-01 00:00:00
abstract::During the last century, two key hypotheses have been proposed to explain apical dominance in plants: auxin promotes the production of a second messenger that moves up into buds to repress their outgrowth, and auxin saturation in the stem inhibits auxin transport from buds, thereby inhibiting bud outgrowth. The recent...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.134783
更新日期:2009-05-01 00:00:00
abstract::Distinct organellar forms of the beta-oxidation enzyme enoyl-coenzyme A (CoA) hydratase were partially purified and characterized from 2-day germinated pea (Pisum sativum L.) seedlings. The purification was accomplished by disruption of purified mitochondria or peroxisomes, (NH(4))(2)SO(4) fractionation, and gel perme...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.564
更新日期:1991-02-01 00:00:00
abstract::Hydroperoxide lyase (HPLS) activity in soybean (Glycine max) seed/seedlings, leaves, and chloroplasts of leaves required detergent solubilization for maximum in vitro activity. On a per milligram of protein basis, more HPLS activity was found in leaves, especially chloroplasts, than in seeds or seedlings. The total yi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.3.1059
更新日期:1991-11-01 00:00:00
abstract::Cold acclimation of Arabidopsis thaliana includes the expression of cold-regulated (COR) genes and the accumulation of COR polypeptides. The hydration characteristics of two COR polypeptides, COR6.6 and COR15am, have been determined and their effects on the dehydration-induced liquid crystalline-to-gel and lamellar-to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.1.301
更新日期:1996-05-01 00:00:00
abstract::We have characterized transcripts for nine major intrinsic proteins (MIPs), some of which function as water channels (aquaporins), from the ice plant Mesembryanthemum crystallinum. To determine the cellular distribution and expression of these MIPs, oligopeptide-based antibodies were generated against MIP-A, MIP-B, MI...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.111
更新日期:2000-05-01 00:00:00
abstract::Sensitivity to low concentrations of desaspidin (5 x 10(-7)m) sharply distinguishes the photophosphorylations associated with the photooxidation of water from all other types of photophosphorylation by isolated chloroplasts. Contrary to recent reports in the literature, the effects of desapidin were not altered by cha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.8.1376
更新日期:1966-10-01 00:00:00
abstract::Given the substantial changes in mitochondrial gene expression, the mitochondrial proteome, and respiratory function during rice (Oryza sativa) germination under anaerobic and aerobic conditions, we have attempted to identify changes in mitochondrial membrane transport capacity during these processes. We have assemble...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.162214
更新日期:2010-10-01 00:00:00
abstract::Calmodulin antagonists, trifluoperazine, chlorpromazine, calmidazolium, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), strongly inhibited the electrofusion of barley (Hordeum vulgare L. cv Moor) protoplasts with a marked increase of broken fusion products, after 60 minutes of incubation. W-5, a dechlorinate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.4.1151
更新日期:1986-08-01 00:00:00
abstract::Arbuscular mycorrhiza (AM) is a mutual symbiosis that involves a complex symbiotic interface over which nutrients are exchanged between the plant host and the AM fungus. Dozens of genes in the host are required for the establishment and functioning of the interaction, among them nutrient transporters that mediate the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00310
更新日期:2015-07-01 00:00:00
abstract::Structural and metabolic features of the seedcoats of the developing pea seed indicate that events in the cells of the seedcoats are of major importance in controlling the development of the embryo. Information has been obtained on the distribution of N and P constituents of the seedcoats, embryo sac liquid, and the c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.763
更新日期:1979-11-01 00:00:00
abstract::Plant endosperm cells have a nuclear ratio of two maternal genomes to one paternal genome. This 2 to 1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed us...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.101428
更新日期:2007-10-01 00:00:00
abstract::Osmoregulation in opening stomata of epidermal peels from Vicia faba L. leaves was investigated under a variety of experimental conditions. The K(+) content of stomatal guard cells and the starch content of guard cell chloroplasts were examined with cobaltinitrite and iodine-potassium iodide stains, respectively; stom...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.887
更新日期:1988-11-01 00:00:00
abstract::Four types of phospholipase D (PLD), PLD alpha, beta, gamma, and delta, have been characterized in Arabidopsis, and they display different requirements for Ca(2+), phosphatidylinositol 4,5-bisphosphate (PIP(2)), substrate vesicle composition, and/or free fatty acids. However, all previously cloned plant PLDs contain a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010928
更新日期:2002-03-01 00:00:00
abstract::Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.114868
更新日期:2008-04-01 00:00:00
abstract::Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectiv...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.525
更新日期:1988-11-01 00:00:00