Abstract:
:Four types of phospholipase D (PLD), PLD alpha, beta, gamma, and delta, have been characterized in Arabidopsis, and they display different requirements for Ca(2+), phosphatidylinositol 4,5-bisphosphate (PIP(2)), substrate vesicle composition, and/or free fatty acids. However, all previously cloned plant PLDs contain a Ca(2+)-dependent phospholipid-binding C2 domain and require Ca(2+) for activity. This study documents a new type of PLD, PLD zeta 1, which is distinctively different from previously characterized PLDs. It contains at the N terminus a Phox homology domain and a pleckstrin homology domain, but not the C2 domain. A full-length cDNA for Arabidopsis PLD zeta 1 has been identified and used to express catalytically active PLD in Escherichia coli. PLD zeta 1 does not require Ca(2+) or any other divalent cation for activity. In addition, it selectively hydrolyzes phosphatidylcholine, whereas the other Arabidopsis PLDs use several phospholipids as substrates. PLD zeta 1 requires PIP(2) for activity, but unlike the PIP(2)-requiring PLD beta or gamma, phosphatidylethanolamine is not needed in substrate vesicles. These differences are described, together with a genomic analysis of 12 putative Arabidopsis PLD genes that are grouped into alpha, beta, delta, gamma, and zeta based on their gene architectures, sequence similarities, domain structures, and biochemical properties.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Qin C,Wang Xdoi
10.1104/pp.010928subject
Has Abstractpub_date
2002-03-01 00:00:00pages
1057-68issue
3eissn
0032-0889issn
1532-2548journal_volume
128pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The response of the membrane potential to HCO(3) (-) supply has been studied in the cyanobacterium Anabaena variabilis strain M-3 under various conditions. Changes in potential were followed with the aid of the lipophilic cation tetraphenyl phosphonium bromide.Addition of HCO(3) (-) to CO(2)-depleted cells resulted in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.4.978
更新日期:1982-04-01 00:00:00
abstract::The distribution of activity of a potassium-stimulated ATPase associated with the plasma membrane was determined in 4-day-old soybean roots. Changes in protein-based specific activity of the enzyme coincided with developmental changes in the root. Activity was low in the region of the root cap, increased to a maximum ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1187
更新日期:1979-06-01 00:00:00
abstract::Serine hydroxymethyltransferase from mammalian and bacterial sources is a pyridoxal-5'-phosphate-containing enzyme, but the requirement of pyridoxal-5'-phosphate for the activity of the enzyme from plant sources is not clear. The specific activity of serine hydroxymethyltransferase isolated from mung bean (Vigna radia...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.351
更新日期:1991-02-01 00:00:00
abstract::Upon illumination of dark-grown maize seedlings (5 days old) with incandescent light, there occurred a nearly simultaneous increase, after a certain lag period, in the activities of enzymes engaged in the C(4) pathway and the Calvin-Benson cycle. The light-induced biosynthesis of chlorophyll (a and b) precedes the inc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.2.198
更新日期:1980-02-01 00:00:00
abstract::Intact amyloplasts from potato (Solanum tuberosum L.) were used to study starch biosynthesis and phosphorylation. Assessed by the degree of intactness and by the level of cytosolic and vacuolar contamination, the best preparations were selected by searching for amyloplasts containing small starch grains. The isolated,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.2.455
更新日期:1999-02-01 00:00:00
abstract::The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control lin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.2.823
更新日期:2000-10-01 00:00:00
abstract::Thermostability of the photosynthetic apparatus of abscisic acid (ABA)-treated seedlings of barley (Hordeum vulgare) was studied by light-scattering and by fluorescence measurements of isolated chloroplasts. ABA treatment markedly decreased heat damage of the chloroplast ultrastructure; an exogenous ABA concentration ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1228
更新日期:1992-04-01 00:00:00
abstract::A rapid and simple, large-scale method for the purification of DNA-dependent RNA polymerase III (EC 2.7.7.6) from wheat germ is presented. The method involves enzyme extraction at low ionic strength, polyethyleneimine fractionation, (NH(4))(2)SO(4) precipitation, and chromatography on DEAE-Sepharose CL-6B, DEAE-cellul...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.3.438
更新日期:1981-03-01 00:00:00
abstract::Light is a major environmental factor required for stomatal opening. Blue light (BL) induces stomatal opening in higher plants as a signal under the photosynthetic active radiation. The stomatal BL response is not present in the fern species of Polypodiopsida. The acquisition of a stomatal BL response might provide co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00134
更新日期:2015-10-01 00:00:00
abstract::Ultraviolet-B (UV-B) photons can cause substantial cellular damage in biomolecules, as is well established for DNA. Because RNA has the same absorption spectrum for UV as DNA, we have investigated damage to this cellular constituent. In maize (Zea mays) leaves, UV-B radiation damages ribosomes by crosslinking cytosoli...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.047043
更新日期:2004-10-01 00:00:00
abstract::Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step toward a detailed understanding of this importa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.126375
更新日期:2008-11-01 00:00:00
abstract::While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01347
更新日期:2016-12-01 00:00:00
abstract::Cotyledons of cotton (Gossypium hirsutum L.) seedlings grown under a photoperiod of 12 hour darkness and 12 hour light showed daily oscillations in ethylene evolution. The rate of ethylene evolution began to increase toward the end of the dark period and reached a maximum rate during the first third of the light perio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.2.493
更新日期:1984-06-01 00:00:00
abstract::Bipyridinium herbicides, including paraquat and diquat, are believed to act by generating highly reactive, oxygen-centered free radicals within chloroplasts when treated plants are exposed to sunlight. This hypothesis has not yet been confirmed by direct chemical measurements of specific free radicals. We studied para...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.4.1267
更新日期:1989-08-01 00:00:00
abstract::Phytochrome wild-type gene-[beta]-glucuronidase (PHY-GUS) gene fusions were used in transgenic Arabidopsis to compare the activity levels and light regulation of the PHYA and PHYB promoters and to identify the photoreceptors mediating this regulation. In dark-grown seedlings, both promoters are 4-fold more active in s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.2.523
更新日期:1995-02-01 00:00:00
abstract::Flavones are important copigments found in the flowers of many higher plants and play a variety of roles in plant adaptation to stress. In Medicago species, flavones also act as signal molecules during symbiotic interaction with the diazotropic bacterium Sinorhizobium meliloti. They are the most potent nod gene induce...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.095018
更新日期:2007-06-01 00:00:00
abstract::Movement of [(14)C]kinetin and [(14)C]gibberellic acid was examined in cotton (Gossypium hirsutum L.) cotyledonary petiole sections independent of label uptake or exit from the tissue. Sections 20 millimeters in length were taken from well watered, stressed, and poststressed plants. Transport capacity was determined u...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.1.152
更新日期:1979-01-01 00:00:00
abstract::Superoxide dismutases (SODs) play a key role in the cellular defense against reactive oxygen species. To study the transcriptional regulation at the cellular level, the promoter of the Nicotiana plumbaginifolia cytosolic gene encoding Cu/ZnSOD (SODCc) was fused to the beta-glucuronidase (GUS) reporter gene (gusA) and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.3.873
更新日期:1994-03-01 00:00:00
abstract::The influence of light on asexual fruiting and mycelial growth of Phycomyces blakesleeanus Burgeff was studied by means of fruiting body counts and size measurements in cultures on solid media under varied incubation conditions. Five types of photoresponses were shown by ATCC Strain 8743a: (a) photoinduction of giant ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.3.570
更新日期:1973-03-01 00:00:00
abstract::The effects of the plant growth substances indoleacetic acid (IAA) and fusicoccin on the transmembrane potential of Avena coleoptile cells (at 27-29 C) were studied. Fusicoccin caused hyperpolarization of the membrane potential which started after a lag of less than 20 seconds, and which on average reached -49 mv at a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.3.395
更新日期:1977-03-01 00:00:00
abstract::Analysis of a mutation in cotton (Gossypium hirsutum), which is maternally inherited, revealed that the completely white sectors of leaves were deficient in the 70S class ribosomes, whereas the yellow sectors exhibited the same level of the latter as in the green leaves. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.6.1138
更新日期:1973-06-01 00:00:00
abstract::Endosperm liquid bathes the embryo of Phaseolus vulgaris from the heart stage through the late cotyledon state. This liquid was aspirated from many ovules of the same stage, pooled, and analyzed for the following constituents and parameters: [List: see text]. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.3.454
更新日期:1973-03-01 00:00:00
abstract::Short-term ion uptake into roots of Limnobium stoloniferum was followed extracellularly with ion selective macroelectrodes. Cytosolic or vacuolar pH, together with the electrical membrane potential, was recorded with microelectrodes both located in the same young root hair. At the onset of chloride, phosphate, and nit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.4.1561
更新日期:1990-12-01 00:00:00
abstract::Receptor-like kinases (RLKs) are the largest family of proteins in plants and are responsible for perceiving the vast majority of extracellular stimuli. Thus, RLKs function in diverse processes, including sensing pathogen attacks, regulating symbiotic interactions, transducing hormone and peptide signals, and monitori...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01700
更新日期:2018-06-01 00:00:00
abstract::The elongation rate of cowpea epicotyls from whole cowpea (Vigna sinensis) seedlings and derooted and debladed plants (explants) increased after the main light period (8-hour duration) was extended with either continuous low intensity tungsten light or brief (5 minutes) far-red (FR) irradiation. This end-of-day FR eff...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.1.212
更新日期:1987-09-01 00:00:00
abstract::Reactive oxygen species act as signaling molecules but can also directly provoke cellular damage by rapidly oxidizing cellular components, including lipids. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry-based quantitative method that allowed us to discriminate b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.125690
更新日期:2008-10-01 00:00:00
abstract::Extracts from various parts of Phaseolus vulgaris L. var. Pinto plants were found to exhibit cytokinin activity with the highest levels present in extracts of the fruit tissue. To separate the cytokinins present in the fruit, extracts were chromatographed in several solvent systems. Bioassays of chromatograms indicate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.3.320
更新日期:1971-09-01 00:00:00
abstract::The light-harvesting complex (LHC) of photosystem II is composed of several different pigment-binding apoproteins. We have identified a cDNA clone LHCIIa-1 encoding the 31-kilodalton LHC IIa (CP29, Chl a/b-P1) apoprotein of barley (Hordeum vulgare). Direct protein microsequencing of an internal peptide fragment from t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.1.238
更新日期:1992-01-01 00:00:00
abstract::Nitrate reductase from wheat (Triticum aestivum L. cv Bindawarra) leaves is inactivated by pretreatment with NADH, in the absence of nitrate, a 50% loss of activity occurring in 30 minutes at 25 degrees C with 10 micromolar NADH. Nitrate (50 micromolar) prevented inactivation by 10 micromolar NADH while cyanide (1 mic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.3.582
更新日期:1983-03-01 00:00:00
abstract::Antibodies were raised against carrot (Daucus carota) cell wall beta-fructosidase that was either in a native configuration (this serum is called anti-betaF(1)) or chemically deglycosylated (anti-betaF(2)). The two antisera had completely different specificities when tested by immunoblotting. The anti-betaF(1) serum r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.3.1182
更新日期:1989-07-01 00:00:00