Primary structure of a proline-rich zein and its cDNA.

Abstract:

:Eighty-five cDNA clones for gamma-zein (proline-rich zein) from a cDNA expression library were isolated using specific antibody and cDNA probes. Nucleotide sequences of seven independent clones were determined and found to be identical in regions where they overlapped. The primary structure of the mature protein, determined from the sequence of one near full-length clone, consists of 204 amino acids. It has a molecular weight of 21,824 daltons, about 5 kilodaltons less than that estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The N-terminal one-half of the sequence contained eight essentially identical tandem repeats of the hexapeptide Pro-Pro-Pro-Val-His-Leu and two of the octapeptide Gln-Pro-His-Pro-Cys-Pro-Cys-Gln. The codon specifying the third proline in the hexapeptide repeating units is identical (CCG) in all of the eight repeats. The coding region has a very high G-C content (69.8%). The multiple charge components of gamma-zein detected by isoelectric focusing do not seem to be encoded by members of a multigene family. Moreover, it was found that the codon preference in gamma-zein is, in fact, the base preference in the wobble position. A codon usage value was devised to express this phenomenon.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Wang SZ,Esen A

doi

10.1104/pp.81.1.70

subject

Has Abstract

pub_date

1986-05-01 00:00:00

pages

70-4

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

81

pub_type

杂志文章
  • Stomatal responses to flooding of the intercellular air spaces suggest a vapor-phase signal between the mesophyll and the guard cells.

    abstract::Flooding the intercellular air spaces of leaves with water was shown to cause rapid closure of stomata in Tradescantia pallida, Lactuca serriola, Helianthus annuus, and Oenothera caespitosa. The response occurred when water was injected into the intercellular spaces, vacuum infiltrated into the intercellular spaces, o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.157685

    authors: Sibbernsen E,Mott KA

    更新日期:2010-07-01 00:00:00

  • Expression of a bacterial ice nucleation gene in plants.

    abstract::We have introduced an ice nucleation gene (inaZ) from Pseudomonas syringae pv. syringae into Nicotiana tabacum, a freezing-sensitive species, and Solanum commersonii, a freezing-tolerant species. Transformants of both species showed increased ice nucleation activity over untransformed controls. The concentration of ic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1730

    authors: Baertlein DA,Lindow SE,Panopoulos NJ,Lee SP,Mindrinos MN,Chen TH

    更新日期:1992-12-01 00:00:00

  • Redox regulation of Arabidopsis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.

    abstract::The cDNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Arabidopsis encodes a polypeptide with an amino-terminal signal sequence for plastid import. A cDNA fragment encoding the processed form of the enzyme was expressed in Escherichia coli. The resulting protein was purified to electrophoretic homogeneit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.002626

    authors: Entus R,Poling M,Herrmann KM

    更新日期:2002-08-01 00:00:00

  • Inhibition of Auxin-induced Deoxyribonucleic Acid Synthesis and Chromatin Activity by 5-Fluorodeoxyuridine in Soybean Hypocotyl.

    abstract::Rootless soybean (Glycine max) seedlings were used as a test system to examine the action of auxin on chromatin-directed RNA synthesis. Chromatin from the basal tissue of rootless seedlings (both control and auxin-treated) had RNA synthetic capacity similar to that of chromatin from comparably treated intact seedlings...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.5.606

    authors: Holm RE,Key JL

    更新日期:1971-05-01 00:00:00

  • Starch-synthesizing Enzymes in the Endosperm and Pollen of Maize.

    abstract::Two mutations, amylose-extender and waxy, which affect the proportion of amylose and amylopectin of starch synthesized in the endosperm of maize (Zea mays L.) seeds, are also expressed in the pollen. However, most mutations that affect starch synthesis in the maize endosperm are not expressed in the pollen. In an atte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.312

    authors: Bryce WH,Nelson OE

    更新日期:1979-02-01 00:00:00

  • Iron acquisition by phytosiderophores contributes to cadmium tolerance.

    abstract::Based on the ability of phytosiderophores to chelate other heavy metals besides iron (Fe), phytosiderophores were suggested to prevent graminaceous plants from cadmium (Cd) toxicity. To assess interactions between Cd and phytosiderophore-mediated Fe acquisition, maize (Zea mays) plants were grown hydroponically under ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.094474

    authors: Meda AR,Scheuermann EB,Prechsl UE,Erenoglu B,Schaaf G,Hayen H,Weber G,von Wirén N

    更新日期:2007-04-01 00:00:00

  • Homomeric interaction of AtVSR1 is essential for its function as a vacuolar sorting receptor.

    abstract::Vacuolar sorting receptors, BP80/VSRs, play a critical role in vacuolar trafficking of soluble proteins in plant cells. However, the mechanism of action of BP80 is not well understood. Here, we investigate the action mechanism of AtVSR1, a member of BP80 proteins in Arabidopsis (Arabidopsis thaliana), in vacuolar traf...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.159814

    authors: Kim H,Kang H,Jang M,Chang JH,Miao Y,Jiang L,Hwang I

    更新日期:2010-09-01 00:00:00

  • 5-Aminolevulinate Synthesis in Permeabilized Filaments of the Blue-Green Alga Anabaena variabilis.

    abstract::Filaments of the blue-green alga Anabaena variabilis permeabilized by dimethylsulfoxide (DMSO) produce increased amounts of 5-aminolevulinate in the presence of levulinic acid. The metabolic activity of the filaments remains unperturbed in the presence of up to 7.5% (v/v) DMSO. Studies utilizing DMSO-permeabilized fil...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.1.200

    authors: Avissar YJ

    更新日期:1983-05-01 00:00:00

  • The requirement for sodium as a micronutrient by species having the c(4) dicarboxylic photosynthetic pathway.

    abstract::Six species having characteristics of plants with the C(4) dicarboxylic photosynthetic pathway, Echinochloa utilis L. Ohwi et Yabuno (Japanese millet), Cynodon dactylon L. (Bermuda grass), Kyllinga brevifolia Rottb., Amaranthus tricolor L. cv. Early splendour, Kochia childsii Hort., and Portulaca grandiflora Hook (ros...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.5.794

    authors: Brownell PF,Crossland CJ

    更新日期:1972-05-01 00:00:00

  • The Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation.

    abstract::Induction of secreted acid phosphatase (APase) is a universal response of higher plants to phosphate (Pi) limitation. These enzymes are thought to scavenge Pi from organophosphate compounds in the rhizosphere and thus to increase Pi availability to plants when Pi is deficient. The tight association of secreted APase w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.183723

    authors: Wang L,Li Z,Qian W,Guo W,Gao X,Huang L,Wang H,Zhu H,Wu JW,Wang D,Liu D

    更新日期:2011-11-01 00:00:00

  • Five geranylgeranyl diphosphate synthases expressed in different organs are localized into three subcellular compartments in Arabidopsis.

    abstract::Geranylgeranyl diphosphate (GGPP) is the precursor for the biosynthesis of gibberellins, carotenoids, chlorophylls, isoprenoid quinones, and geranylgeranylated proteins in plants. There is a small gene family for GGPP synthases encoding five isozymes and one related protein in Arabidopsis, and all homologs have a puta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.4.1045

    authors: Okada K,Saito T,Nakagawa T,Kawamukai M,Kamiya Y

    更新日期:2000-04-01 00:00:00

  • Ethylene-induced lateral expansion in etiolated pea stems : the role of Acid secretion.

    abstract::Ethylene-induced inhibition of elongation and promotion of lateral expansion in the stems of etiolated pea (Pisum sativum L. var Alaska) seedlings is not associated with any alteration of auxin-stimulated proton extrusion. Indeed, lateral expansion in response to ethylene apparently requires an acidified wall since it...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.413

    authors: Taiz L,Rayle DL,Eisinger W

    更新日期:1983-10-01 00:00:00

  • Light sensitivity of na, rb, and k absorption by different tissues of bean leaves.

    abstract::Autoradiographs of (22)Na-loaded bean (Phaseolus vulgaris L. cv. ;Brittle Wax') leaf slices showed that most of the tracer was concentrated in vascular tissue. Rubidium-86 was uniformly distributed in slices that had been incubated in darkness; after incubation in the light many small dark spots appeared on the autora...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.6.978

    authors: Jacoby B

    更新日期:1975-06-01 00:00:00

  • Auxin transport in suspension-cultured soybean root cells : I. Characterization.

    abstract::The kinetic parameters of auxin transport in suspension-cultured soybean (Glycine max [L.] Merr.) root cells were investigated. The same processes that are responsible for polar indoleacetic acid (IAA) transport in other plant tissues were found to occur in soybean root cells. These include (a) passive diffusion of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.1.184

    authors: Loper MT,Spanswick RM

    更新日期:1991-05-01 00:00:00

  • Membrane rupture is the common cause of damage to chloroplast membranes in leaves injured by freezing or excessive wilting.

    abstract::The effects of freezing and desiccation of spinach leaves (Spinacia oleracea L. cv Yates) on the thylakoid membranes were assessed using antibodies specific for thylakoid membrane proteins. The peripheral part of the chloroplast coupling factor ATPase (CF1) was used as a molecular marker for chemical membrane damage b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.251

    authors: Hincha DK,Höfner R,Schwab KB,Heber U,Schmitt JM

    更新日期:1987-02-01 00:00:00

  • Purification and Partial Characterization of a Genetically-Defined Superoxide Dismutase (SOD-1) Associated with Maize Chloroplasts.

    abstract::The chloroplast-associated form of superoxide dismutase from maize (Zea mays L.) (SOD-1) has been purified by a stepwise procedure consisting of (NH(4))(2)SO(4) fractionation, G-100 Sephadex gel filtration, DEAE-Sephacel chromatography, and hydroxylapatite chromatography. This procedure resulted in a single band on so...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.31

    authors: Baum JA,Chandlee JM,Scandalios JG

    更新日期:1983-09-01 00:00:00

  • Distinct Mechanisms of the ORANGE Protein in Controlling Carotenoid Flux.

    abstract::β-Carotene adds nutritious value and determines the color of many fruits, including melon (Cucumis melo). In melon mesocarp, β-carotene accumulation is governed by the Orange gene (CmOr) golden single-nucleotide polymorphism (SNP) through a yet to be discovered mechanism. In Arabidopsis (Arabidopsis thaliana), OR incr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01256

    authors: Chayut N,Yuan H,Ohali S,Meir A,Sa'ar U,Tzuri G,Zheng Y,Mazourek M,Gepstein S,Zhou X,Portnoy V,Lewinsohn E,Schaffer AA,Katzir N,Fei Z,Welsch R,Li L,Burger J,Tadmor Y

    更新日期:2017-01-01 00:00:00

  • Disruption of the actin cytoskeleton results in the promotion of gravitropism in inflorescence stems and hypocotyls of Arabidopsis.

    abstract::The actin cytoskeleton is hypothesized to play a major role in gravity perception and transduction mechanisms in roots of plants. To determine whether actin microfilaments (MFs) are involved in these processes in stem-like organs, we studied gravitropism in Arabidopsis inflorescence stems and hypocotyls. Localization ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010804

    authors: Yamamoto K,Kiss JZ

    更新日期:2002-02-01 00:00:00

  • Effects of drought on gene expression in maize reproductive and leaf meristem tissue revealed by RNA-Seq.

    abstract::Drought stress affects cereals especially during the reproductive stage. The maize (Zea mays) drought transcriptome was studied using RNA-Seq analysis to compare drought-treated and well-watered fertilized ovary and basal leaf meristem tissue. More drought-responsive genes responded in the ovary compared with the leaf...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.200444

    authors: Kakumanu A,Ambavaram MM,Klumas C,Krishnan A,Batlang U,Myers E,Grene R,Pereira A

    更新日期:2012-10-01 00:00:00

  • The DEK1 Calpain Linker Functions in Three-Dimensional Body Patterning in Physcomitrella patens.

    abstract::The DEFECTIVE KERNEL1 (DEK1) calpain is a conserved 240-kD key regulator of three-dimensional body patterning in land plants acting via mitotic cell plane positioning. The activity of the cytosolic C-terminal calpain protease is regulated by the membrane-anchored DEK1 MEM, which is connected to the calpain via the 600...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00925

    authors: Johansen W,Ako AE,Demko V,Perroud PF,Rensing SA,Mekhlif AK,Olsen OA

    更新日期:2016-10-01 00:00:00

  • Polyphosphoinositide Phospholipase C in Plasma Membranes of Wheat (Triticum aestivum L.) : Orientation of Active Site and Activation by Ca and Mg.

    abstract::Polyphosphoinositide-specific phospholipase C activity was present in plasma membranes isolated from different tissues of several higher plants. Phospholipase C activities against added phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP(2)) were further characterized in plasma membra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.3.1296

    authors: Pical C,Sandelius AS,Melin PM,Sommarin M

    更新日期:1992-11-01 00:00:00

  • Nitric oxide modulates the activity of tobacco aconitase.

    abstract::Recent evidence suggests an important role for nitric oxide (NO) signaling in plant-pathogen interactions. Additional elucidation of the role of NO in plants will require identification of NO targets. Since aconitases are major NO targets in animals, we examined the effect of NO on tobacco (Nicotiana tabacum) aconitas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.2.573

    authors: Navarre DA,Wendehenne D,Durner J,Noad R,Klessig DF

    更新日期:2000-02-01 00:00:00

  • New light on the cholodny-went theory.

    abstract::The existence of different auxin sensitivities in epidermal and subepidermal tissues (KV Thimann, CL Schneider 1938 Plant Physiol 25: 627-641) suggests a refinement to the Cholodny-Went theory which overcomes some of the difficulties associated with it. A model is presented to account for the inverse tropic responses ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.568

    authors: Macdonald IR,Hart JW

    更新日期:1987-07-01 00:00:00

  • Genome-wide detection of condition-sensitive alternative splicing in Arabidopsis roots.

    abstract::Iron (Fe) deficiency is a world-wide nutritional disorder in both plants and humans, resulting from its restricted bioavailability for plants and, subsequently, low Fe concentration in edible plant parts. Plants have evolved sophisticated mechanisms to alleviate Fe deficiency, with the aim of recalibrating metabolic f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.217778

    authors: Li W,Lin WD,Ray P,Lan P,Schmidt W

    更新日期:2013-07-01 00:00:00

  • incurvata13, a novel allele of AUXIN RESISTANT6, reveals a specific role for auxin and the SCF complex in Arabidopsis embryogenesis, vascular specification, and leaf flatness.

    abstract::Auxin plays a pivotal role in plant development by modulating the activity of SCF ubiquitin ligase complexes. Here, we positionally cloned Arabidopsis (Arabidopsis thaliana) incurvata13 (icu13), a mutation that causes leaf hyponasty and reduces leaf venation pattern complexity and auxin responsiveness. We found that i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.207779

    authors: Esteve-Bruna D,Pérez-Pérez JM,Ponce MR,Micol JL

    更新日期:2013-03-01 00:00:00

  • Sucrose phosphate synthase, a key enzyme for sucrose biosynthesis in plants: protein purification from corn leaves and immunological detection.

    abstract::We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS pol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.473

    authors: Bruneau JM,Worrell AC,Cambou B,Lando D,Voelker TA

    更新日期:1991-06-01 00:00:00

  • Morphogenesis in Selaginella: II. Auxin Transport in the Root (Rhizophore).

    abstract::The rhizophore of Selaginella willdenovii Baker develops from the ventral angle meristem. The morphological nature of this organ has been in dispute. The purpose of this investigation was to obtain physiological evidence to support the contention that the rhizophore is a root and not a shoot. This was accomplished by ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.5.738

    authors: Wochok ZS

    更新日期:1974-05-01 00:00:00

  • Characterization of glyoxysomes from castor bean endosperm.

    abstract::Electron micrographs are presented which establish the identity of the components of the 3 major bands observed after sucrose density centrifugation of the crude particulate fraction from the endosperm of germinating castor bean seedlings. These are: mitochondria (density 1.19 g/cc), proplastids (density 1.23 g/cc) an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.5.705

    authors: Breidenbach RW,Kahn A,Beevers H

    更新日期:1968-05-01 00:00:00

  • Polysome Formation in Light-controlled Dormancy.

    abstract::Lettuce (Lactuca sativa) seeds var. Grand Rapids could be maintained many weeks in the dark without germination. Following illumination with white light, a gradual increase in polyribosome population up to the time of germination was demonstrated by sucrose gradient centrifugation. Polysomes could not be detected in i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.6.671

    authors: Mitchell RC,Villiers TA

    更新日期:1972-12-01 00:00:00

  • Amyloplast sedimentation dynamics in maize columella cells support a new model for the gravity-sensing apparatus of roots.

    abstract::Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic streaming. Following 90 degrees rotation of the root, the statoliths moved downward along the distal wall and then spread out al...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.1045

    authors: Yoder TL,Zheng HQ,Todd P,Staehelin LA

    更新日期:2001-02-01 00:00:00