Regulation of tonoplast k channels by voltage in the range of physiological electric potentials.

Abstract:

:The presence of tonoplast ion channels regulated by voltage in the physiological range of transtonoplast electric potential was studied in isolated vacuoles from Acer pseudoplatanus cultured cells. In symmetrical KCl or K-gluconate depolarizing pulses induced instantaneously developing, decaying outward currents, while in symmetrical tetramethylammonium chloride these currents were absent. The outward currents were reduced if the depolarizations were applied from a holding potential of +30 millivolts and increased upon depolarizations from a holding potential of -30 millivolts and even more from a holding potential of -50 millivolts. These results indicate that the outward currents are due to K(+) movement through channels which are open around 0 millivolt and close at positive potentials. These K(+) channels, regulated in the range of the physiological electric potentials reported for the vacuoles in situ, are likely the same K(+) channels activated by hyperpolarizations which we have previously described (R Colombo, R Cerana, P Lado, A Peres [1988] J Membr Biol 103: 227-236).

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Colombo R,Cerana R,Lado P

doi

10.1104/pp.93.1.350

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

350-2

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

93

pub_type

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