Abstract:
:We have investigated the effects of hCG and estradiol on the time course of the inhibition of C17-20-lyase and the depletion of the estradiol cytosol receptor in testicular tissue of male rats. In hCG-treated animals the first significant decrease in C17-20-lyase activity was observed 16 h after injection of the hormone. The C17-20-lyase activity decreased further during the following 56 hours. Estradiol-cytosol receptor levels were significantly decreased 7 hours after the hCG injection, and after a nadir at 16 hours increased to 130% of control values at 72 hours. In contrast, in the estradiol-treated animals the first significant inhibition of C17-20-lyase activity could be observed only after 24 h, while a 95% depletion of estradiol-cytosol receptor levels was observed already within 1 h after administration of the hormone. These results demonstrate that a rapid estradiol-induced depletion of the estradiol receptor as such does not necessarily result in a subsequent immediate inhibition of the C17-20-lyase. The rapid inhibition of this enzyme after hCG indicates, therefore, that other mechanisms are involved.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Brinkmann A,Leemborg I,Rommerts F,van der Molen Hdoi
10.1210/endo-110-5-1834subject
Has Abstractpub_date
1982-05-01 00:00:00pages
1834-6issue
5eissn
0013-7227issn
1945-7170journal_volume
110pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::This study was undertaken to identify uterine insulin-like growth factor-I (IGF-I) receptors and examine the regulation of these receptors throughout the estrous cycle and after 17 beta-estradiol (E2) administration to immature rats. We have demonstrated type I IGF receptors in crude uterine membranes by binding and c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-597
更新日期:1989-08-01 00:00:00
abstract::Endocrine regulation of uterine biology is critical for embryo receptivity and human reproduction. Uterine endometrium depends on extrinsic sex steroid input and hence likely has mechanisms that enable adaptation to hormonal variation. Emerging evidence suggests that sex steroid bioavailability in the endometrium is d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1139
更新日期:2014-11-01 00:00:00
abstract::Recent observations suggest a role for interleukin-1 (IL-1), a polypeptide product of macrophage/monocytic cells, in the immune-mediated destruction of pancreatic islet beta-cells observed in type 1 diabetes. In this study, we investigated the effects of IL-1 on both alpha- and beta-cell secretory functions in rat isl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2393
更新日期:1988-06-01 00:00:00
abstract::Recent work has led to the hypothesis that kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the arcuate nucleus play a key role in GnRH pulse generation, with kisspeptin driving GnRH release and neurokinin B (NKB) and dynorphin acting as start and stop signals, respectively. In this study, we tested this hypothesis...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1331
更新日期:2013-11-01 00:00:00
abstract::One of the hypotheses attempting to explain the etiology of the human disease X-linked hypophosphatemia (XLH) posits renal hypersensitivity to parathyroid hormone (PTH). These studies were designed to test this hypothesis in vivo, using the hemizygous hypophosphatemic (Hyp/Y) mouse as an animal model for XLH. Vehicle ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-3-1030
更新日期:1982-03-01 00:00:00
abstract::The testis contains many peptides originally described as originating in the central nervous system. The physiological function of these factors in the testis is generally unknown. We previously reported that the rat testis contains both a peptide with GH-releasing hormone-like immunoactivity (tGHRH-LI) and a mRNA spe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2336
更新日期:1990-11-01 00:00:00
abstract::Newborn male rats were treated SC with dexamethasone phosphate (100 microgram) or dexamethasone acetate (100 microgram) on postnatal day 1, dexamethasone phosphate (5.0 microgram) opn postnatal days 1, 2, 3, or the corresponding drug vehicle. All animals were sacrificed 65-70 days later at previously determined daily ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-1055
更新日期:1981-03-01 00:00:00
abstract::GH and IGF-I are potent regulators of muscle growth and function. Although IGF-I is known to mediate many of the effects of GH, it is not yet clear whether all effects of GH are completely dependent on the IGF-I system. To evaluate the biological effects of the GH/IGF-I axis on muscle growth, we administrated recombin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0906
更新日期:2005-04-01 00:00:00
abstract::In rat pancreatic islets, the effect of old age (24-month-old) on [125I]insulin binding, glucose-induced insulin release and inhibition of insulin secretion by exogenous insulin were studied. The results were compared with corresponding data obtained from young (3-month-old) rats. Specifically bound [125I]insulin in i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-6-2147
更新日期:1983-06-01 00:00:00
abstract::Covalent adduction of a nitrosyl group to cysteines [S-nitrosylation (S-NO)] is emerging as a key route for nitric oxide (NO) to directly modulate protein functions. Here, we studied the effects of estrogens on endothelial protein S-NO and analyzed the nitrosyl-proteomes by biotin/CyDye switch technique combined with ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1356
更新日期:2010-08-01 00:00:00
abstract::In fish spermatogenesis, the main action of progestins is generally regarded as the induction of sperm maturation. Our previous in vitro study demonstrated that a progestin, 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP), induced the initiation of meiosis in spermatogenesis in the Japanese eel (Anguilla japonica). In ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0391
更新日期:2006-11-01 00:00:00
abstract::Nuclear entry of proteins the size of the glucocorticoid and thyroid hormone receptors appears to be mediated by an interaction of nuclear localization signals (NLSs) within the proteins and specific NLS-binding proteins. NLSs have been identified in the hinge region of both receptors. We have identified the cellular ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.3.8440170
更新日期:1993-03-01 00:00:00
abstract::A new member of the connexin family was isolated from the porcine ovary. The amino acid sequence deduced from the nucleotide sequence of genomic as well as complementary DNA clones predicted the reading frame encoding the 60-kDa protein product, indicating the largest molecular mass among the connexin family genes ana...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5665
更新日期:1998-01-01 00:00:00
abstract::TRH is a potent stimulator of pituitary TSH release, but its function in the physiological regulation of thyroid activity is still controversial. The purpose of the present study was to investigate TRH and catecholamine secretion into hypophysial portal blood of hypothyroid and hyperthyroid rats, and in rats bearing p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-523
更新日期:1988-07-01 00:00:00
abstract::Leptin enhances the glucose utilization in most insulin target tissues and paradoxically decreases it in white adipose tissue (WAT), but knowledge of the mechanisms underlying the inhibitory effect of central leptin on the insulin-dependent glucose uptake in WAT is limited. After 7 d intracerebroventricular leptin tre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0858
更新日期:2011-04-01 00:00:00
abstract::The present studies investigated whether insulin inhibits the stimulatory effect of dexamethasone (DEX) on angiotensinogen (ANG) gene expression and induction of hypertrophy in rat immortalized renal proximal tubular cells (IRPTCs) in a high-glucose milieu. Rat IRPTCs were cultured in monolayer. ANG and ANG mRNA expre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220408
更新日期:2002-12-01 00:00:00
abstract::The rapid, nongenomic effects of estrogen are increasingly recognized as playing an important role in several aspects of estrogen action. Rapid activation of the mitogen-activated protein kinase (MAPK) signaling pathway by estrogen is among the more recently identified of these effects. To explore the role of estrogen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.6.8071
更新日期:2001-06-01 00:00:00
abstract::The binding of [125I]iodo-low density lipoprotein ([125I]iodo-LDL) to isolated membranes was determined for the outer (glomerulosa/fasciculata) and inner (reticularis) zones of the guinea pig adrenal cortex. Binding of [125I]iodo-LDL to membranes from both zones was found to be highly specific and dependent on the pre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-653
更新日期:1986-02-01 00:00:00
abstract::Kisspeptin regulates reproductive events, including puberty and ovulation, primarily via GnRH neurons. Prolonged treatment of prepubertal striped bass females with kisspeptin (Kiss) 1 or Kiss2 peptides failed to enhance puberty but suggested a gnrh-independent pituitary control pathway. Kiss2 inhibited, but Kiss1 stim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1894
更新日期:2014-05-01 00:00:00
abstract::We have found that microwave (MW) stabilization greatly improves detection of the estrogen receptor (ER) in frozen sections of rhesus monkey oviduct by immunocytochemistry (ICC). Fresh samples of fimbriae were MW-irradiated, frozen, and then cryosectioned. The frozen sections were also MW-treated and then fixed in a p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.9.7649110
更新日期:1995-09-01 00:00:00
abstract::The predisposition of the testis and ovary to primarily synthesize testosterone (T) and estradiol (E2), respectively, is due to gonadal-specific cell types that differentially express the various hydroxysteroid (17beta) dehydrogenase (HSD17B) isoforms. In testes, Leydig cells rely on LH stimulation to maintain express...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0276
更新日期:2006-08-01 00:00:00
abstract::Although tamoxifen (TAM), which is widely used in the treatment of breast cancer, also has a beneficial effect on cisplatin-refractory ovarian cancer, the biological mechanism of this effect has remained obscure. TAM, besides its action as an antiestrogen, also inhibits cell proliferation of estrogen receptor (ER)-neg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0709
更新日期:2004-03-01 00:00:00
abstract::Primary ovarian insufficiency (POI) resulting from ovarian autoimmunity is a poorly understood clinical condition lacking in effective treatments. Understanding the targets of the autoimmune response and induction of ovarian-specific tolerance would allow development of focused therapies to preserve fertility in an at...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0022
更新日期:2011-06-01 00:00:00
abstract::The possible role of the pentose phosphate shunt in thyroid hormone secretion was investigated in vivo and in vitro with mouse thyroid glands. Thyroidal endocytosis in response to TSH, a step of thyroid hormone secretion, was evaluated for its dependency upon the pentose phosphate shunt by using 6-aminonicotinamide (6...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-4-962
更新日期:1975-10-01 00:00:00
abstract::The hypothalamic hormone, GnRH, is released and transported to the anterior pituitary in a pulsatile manner, where it binds to specific high-affinity receptors and regulates gonadotropin biosynthesis and secretion. The frequency of GnRH pulses changes under various physiological conditions, and varying GnRH pulse freq...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.3.4968
更新日期:1997-03-01 00:00:00
abstract::Although leptin is a hormone mainly produced by the adipose tissue, it is also produced by the gastric mucosa and the mammary epithelium and is present in maternal milk. The effects of milk leptin on the neonate are not known. The purpose of the investigation was to evaluate the short-term effects of the administratio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0112
更新日期:2005-06-01 00:00:00
abstract::LH treatment of isolated medium (3- to 7-mm) follicles from porcine ovaries stimulated pregnenolone synthesis by mitochondria isolated from these tissues. This LH stimulation of pregnenolone synthesis was observed regardless of whether exogenous cholesterol was added to the mitochondrial assay system, suggesting that ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-2-588
更新日期:1984-02-01 00:00:00
abstract::Estrogen receptor alpha and beta proteins (ERalpha and ERbeta) at various stages of development of the rat mammary gland were quantified by Western blotting. ERalpha and ERbeta recombinant proteins were used as standards, and their molar concentrations were measured by ligand binding assays. In 3-week-old pregnant, la...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8260
更新日期:2001-07-01 00:00:00
abstract::LH and FSH are composed of a common alpha-subunit and a noncovalently associated hormone-specific beta-subunit. Unassociated beta LH and beta FSH can be retained in the cisternae of endoplasmic reticulum (ER). This phenomenon is particularly evident in gonadotropes of castrated animals where beta-subunits are expresse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867570
更新日期:1995-03-01 00:00:00
abstract::The activity of many endocrine cells is regulated by gamma-aminobutyric acid (GABA). The effects of GABA are mediated by GABAA and/or GABAB receptors. While GABAB receptors in the central nervous system have now been extensively characterized, little is known of the function and pharmacology of GABAB receptors on endo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8381070
更新日期:1993-02-01 00:00:00