Abstract:
:The present studies investigated whether insulin inhibits the stimulatory effect of dexamethasone (DEX) on angiotensinogen (ANG) gene expression and induction of hypertrophy in rat immortalized renal proximal tubular cells (IRPTCs) in a high-glucose milieu. Rat IRPTCs were cultured in monolayer. ANG and ANG mRNA expression in IRPTCs were quantified by a specific RIA for rat ANG and by RT-PCR assay, respectively. A fusion gene containing the full length of the 5'-flanking region of the rat ANG gene linked to a chloramphenicol acetyl transferase reporter gene was introduced into IRPTCs. The level of fusion gene expression was determined by cellular chloramphenicol acetyl transferase enzymatic activity. Cellular hypertrophy was assessed by flow cytometry, cellular p27(Kip1) protein expression, and protein assay. Our results showed that high glucose (i.e. 25 mM) and DEX (10(-7) M) additively stimulated ANG gene expression and induced IRPTC hypertrophy. Insulin inhibited the effect of high glucose and DEX on these parameters. The inhibitory effect of insulin was reversed by PD 98059 (a MAPK inhibitor) but not by wortmannin (a phosphatidylinositol-3-kinase inhibitor). These results demonstrate that insulin is effective in blocking the stimulatory action of high glucose and DEX on ANG gene expression and induction of IRPTC hypertrophy, suggesting its important role in preventing local intrarenal renin-angiotensin system activation and renal proximal tubular cell hypertrophy induced by hyperglycemia and glucocorticoids in vivo.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Zhang SL,Chen X,Wei CC,Filep JG,Tang SS,Ingelfinger JR,Chan JSdoi
10.1210/en.2002-220408subject
Has Abstractpub_date
2002-12-01 00:00:00pages
4627-35issue
12eissn
0013-7227issn
1945-7170journal_volume
143pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Sulfation is an important metabolic pathway facilitating the degradation of thyroid hormone by the type I iodothyronine deiodinase. Different human and rat tissues contain cytoplasmic sulfotransferases that show a substrate preference for 3,3'-diiodothyronine (3,3'-T2) > T3 > rT3 > T4. During investigation of the expr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5743
更新日期:1998-02-01 00:00:00
abstract::Steady state levels of plasma membrane receptors (measured in radioligand assays) result from processes that contribute to the production of receptors (synthesis, recycling, and unmasking) as well as those that contribute to the loss of receptors (degradation, internalization, and inactivation). Accordingly, we have p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2577
更新日期:1990-05-01 00:00:00
abstract::A novel transcript of the GH gene has been identified in ocular tissues of chick embryos. It is, however, unknown whether this transcript (small chicken GH, scGH) is translated. This possibility was therefore assessed. The expression of scGH mRNA was confirmed by RT-PCR, using primers that amplified a 426-bp cDNA of i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1070
更新日期:2007-01-01 00:00:00
abstract::A complementary DNA, uterine-ovarian-specific gene 44 (UO-44), has been isolated from tamoxifen-induced rat uterine complementary DNA library using differential display techniques. UO-44 transcripts are found to be abundant in the uterus and ovary. UO-44 gene expression in the uterus is strictly regulated by estrogens...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8247
更新日期:2001-07-01 00:00:00
abstract::In mice, GH levels rise in response to short-term fasting or starvation (food restriction to 40% of ad libitum intake), similar to that which occurs in humans in response to fasting or anorexia. Recent studies using acyl-ghrelin knockout mice have suggested that the rise in GH during food restriction is essential to s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1849
更新日期:2013-01-01 00:00:00
abstract::A model for the study of testicular function in experimental uremia induced by subtotal nephrectomy in mature male rats is described. Glomerular filtration rate was reduced by 87%, resulting in stable uremia for up to 13 weeks with characteristic biochemical changes and reduction in weight of testes, prostate, seminal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-1974
更新日期:1985-11-01 00:00:00
abstract::Two receptors (CRH receptor type 1 and CRH receptor type 2) have been identified for the stress-induced neuropeptide, CRH and related peptides, urocortin, and urocortin II. We previously found marked down-regulation of cardiac CRH receptor type 2 expression following administration of bacterial endotoxin, lipopolysacc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8342
更新日期:2001-08-01 00:00:00
abstract::The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-3151
更新日期:1990-12-01 00:00:00
abstract::A variety of transcription factors including Wilms tumor gene (Wt-1), steroidogenic factor 1 (Sf-1), dosage-sensitive sex reversal, adrenal hypoplasia congenita on the X-chromosome, Gene 1 (Dax-1), and pre-B-cell transcription factor 1 (Pbx1) have been defined as necessary for regular adrenocortical development. Howev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0681
更新日期:2007-02-01 00:00:00
abstract::The adrenal medulla contains fenestrated capillaries that allow catecholamines and neuropeptides secreted by adrenal chromaffin cells (ACCs) to readily access the circulation. These capillaries may also allow bacterial products to enter the adrenal medulla and interact with ACCs during infection. One potential mediato...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1534
更新日期:2013-01-01 00:00:00
abstract::The FOXO family of forkhead transcription factors stimulates the transcription of target genes involved in many fundamental cell processes, including cell survival, cell cycle progression, DNA repair, and insulin sensitivity. The activity of FOXO proteins is principally regulated by activation of protein kinase B (PKB...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0481
更新日期:2003-12-01 00:00:00
abstract::The developmental patterns of thyroid-stimulating hormone (TSH) was examined in female Long-Evans rats by radioimmunoassay of pituitary and plasma from 5 through 80 days of age. The effects of hypothyroidism on normal TSH levels were examined in animals radiothyroidectomized at birth. Control animals showed a peak in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-1-237
更新日期:1975-07-01 00:00:00
abstract::Porcine serum contains five insulin-like growth factor-binding proteins (IGFBPs), whose regulation has been studied by ligand blotting. To more accurately quantify changes in two specific forms of IGFBP a heterologous RIA for porcine (p) IGFBP-2 was developed, and IGFBP-1 levels were analyzed by immunoblotting. By RIA...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-5-2631
更新日期:1991-11-01 00:00:00
abstract::The role of GH during the ovine estrous cycle is unknown. In this study, pituitary GH mRNA and peripheral serum GH levels were measured in ewes representing the major phases of the estrous cycle. There was a significant rise (P less than 0.05) in both GH mRNA and serum GH concentrations during the follicular phase of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1474
更新日期:1989-09-01 00:00:00
abstract::Luteinizing hormone/chorionic gonadotropin receptor (LHR) expression in the ovary is regulated by a messenger RNA (mRNA) binding protein, which specifically binds to the coding region of LHR mRNA. We have shown that miR-122, a short noncoding RNA, mediates LHR mRNA levels by modulating the expression of LHR mRNA-bindi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03270
更新日期:2018-05-01 00:00:00
abstract::To ascertain if reductions in fetal plasma cortisol cause increases in fetal plasma ACTH, we treated pregnant ewes or their fetuses with aminoglutethimide (10 mg/kg BW) and metyrapone (20 mg/kg BW) and measured the hormonal responses with RIAs. When given to fetuses (n = 9) at 0.90 +/- 0.01 gestation (term-145 days), ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-3-1307
更新日期:1988-09-01 00:00:00
abstract::PTH increases calcium reabsorption exclusively in the distal nephron. Two mechanisms of Ca++ transport through the basolateral membrane (BLM) have been described: the ATP-dependent and the sodium gradient-dependent transport. In the present study, we investigated the effect of PTH and (Bu)2cAMP on these two mechanisms...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-251
更新日期:1991-01-01 00:00:00
abstract::With the rapid changes in lifestyle in modern society, including the high nutritional intake and reduced physical activity, the incidence of metabolic diseases has been increasing year by year. Obstructive sleep apnea syndrome (OSAS) is a sleep disorder, usually characterized by sudden pauses of breathing during sleep...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2018-00248
更新日期:2018-07-01 00:00:00
abstract::Recent studies from our laboratory demonstrated clearly that only a portion of all Sertoli cells secrete transferrin (TF). These findings raised the possibility that differences in the functional type of Sertoli cells from one location to another may account in part for the stage-related variation in TF release along ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-4-1869
更新日期:1991-04-01 00:00:00
abstract::The formation of junctional complexes between adjacent epithelial principal cells leads to formation of the blood-epididymal barrier; this barrier is complete by 21 days of postnatal age. Cadherins are cell surface proteins that mediate intercellular adhesion and are involved in the formation of adherence, gap, and ti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.3.7679970
更新日期:1993-03-01 00:00:00
abstract::Thyroid hormone accelerates energy expenditure (EE) and is critical for cold-induced thermogenesis. To define the metabolic role played by thyroid hormone in the dissipation of calories from diet, hypothyroid mice were studied for 60 d in a comprehensive lab animal monitoring system. Hypothyroidism decreased caloric i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1066
更新日期:2011-09-01 00:00:00
abstract::Bombesin was injected into the cerebral ventricle of male rats anesthetized with urethane to study its effect on plasma levels of immunoreactive somatostatin (IRS) in hypophysial portal and jugular blood. An intraventricular injection of bombesin (0.2 and 2 micrograms/rat) caused a significant and dose-related increas...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-229
更新日期:1981-07-01 00:00:00
abstract::To examine further the relationship between developmental changes in LH release and the onset of puberty, effects of posterior hypothalamic lesions were tested in ovariectomized (OVX), sexually immature female monkeys. In OVX females (n = 3) with sham hypothalamic lesions basal LH levels were suppressed during the pre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-445
更新日期:1988-07-01 00:00:00
abstract::The purpose of this study is to establish a primary LHRH cell culture system using embryonic olfactory placode and to examine whether LHRH cells derived from olfactory placode and the migratory pathway of LHRH neurons mature in vitro. Six monkey fetuses at the ages of E34-E36 were delivered surgically and the area inc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404690
更新日期:1993-11-01 00:00:00
abstract::The concentration-activity profiles for insulin and insulin-like growth factor I (IGF-I; in the presence of and insulin-like growth factor I (IGF-I; in the presence of hydrocortisone and PRL) have been compared in terms of the accumulation of beta-casein mRNA, total casein synthesis, and alpha-lactalbumin and basal ca...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-4-1411
更新日期:1987-04-01 00:00:00
abstract::The effects of FFA on circulating LH and GH concentrations in ovariectomized ewe lambs were investigated. Lambs (n = 14) were weaned at 2.5 months, ovariectomized at 6.5 months, and used at 8.5 months of age. From weaning until day 0 of the experiment, lambs were fed to maintain body weights (23 kg). On day 0, serum F...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-4-1934
更新日期:1990-04-01 00:00:00
abstract::The action of added insulin-like growth factor-I (IGF-I) and dihydrotestosterone (DHT) on steroid 5 alpha-reductase (5 alpha R) activity was studied using primary cultures of rat or human scrotal skin fibroblasts. Agents were added to cultured cells (2 x 10(5) cells) for 2 days, and enzyme activity was measured by the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8344190
更新日期:1993-08-01 00:00:00
abstract::Induction of cyclin proteins is required for progression of cells through the G(1)-S and G(2)-M cell cycle checkpoints and is a primary mechanism by which mitogens regulate cell cycle progression. IGF-I and the epidermal growth factor (EGF)-related ligands are mitogens for mammary epithelial cells in vitro and are ess...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.5.8774
更新日期:2002-05-01 00:00:00
abstract::Mesenchymal stem cells can differentiate into mature chondrocytes, osteoblasts, and adipocytes. Excessive and dysfunctional visceral adipocytes increase upon menopause and importantly contribute to altered metabolism in postmenopausal women. We previously showed both plasma membrane and nuclear estrogen receptors alph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa175
更新日期:2020-11-01 00:00:00
abstract::To address the neural mediation of the eating-inhibitory effect of circulating glucagon-like peptide-1 (GLP-1), we investigated the effects of 1) intra-fourth ventricular infusion of the GLP-1 receptor antagonist exendin-9 or 2) area postrema lesion on the eating-inhibitory effect of intrameal hepatic portal vein (HPV...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1447
更新日期:2014-05-01 00:00:00