Thyroid hormone contamination in crystalline diiodotyrosine.

abstract：:We describe a new multilayer immunoassay element for the determination of haptens in undiluted serum and plasma. Polysaccharide layers are coated onto a plastic base. The signal layer contains an immobilized antibody and a fluorescent-labeled hapten. A second layer, containing a pigment, acts as an optical screen. Sam...

journal_title：Clinical chemistry

authors： Grenner G,Inbar S,Meneghini FA,Long EW,Yamartino EJ Jr,Bowen MS,Blackwood JJ,Padilla AJ,Maretsky D,Staedter M

更新日期：1989-09-01 00:00:00

abstract：:A rapid and precise immunoassay for quantification of total homocysteine in blood samples is presented. The method avoids the use of radioisotopes and chromatographic separations and relies on enzymatic conversion of homocysteine to S-adenosyl-L-homocysteine, followed by quantification of S-adenosyl-L-homocysteine by ...

journal_title：Clinical chemistry

authors： Frantzen F,Faaren AL,Alfheim I,Nordhei AK

更新日期：1998-02-01 00:00:00

abstract：BACKGROUND:Testing for troponin has important clinical value for patients who present with typical symptoms of acute coronary syndromes (ACS) such as chest pain (CP). Much less is known about the value of troponin testing for patients who present with other symptoms of ACS (anginal equivalent symptoms). METHODS:The ut...

journal_title：Clinical chemistry

authors： Ritter D,Lee PA,Taylor JF,Hsu L,Cohen JD,Chung HD,Virgo KS

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:Measurements of the ionic current flowing through nanometer-scale pores (nanopores) have been used to analyze single DNA and RNA molecules, with the ultimate goal of achieving ultrafast DNA sequencing. However, attempts at purely electronic measurements have not achieved the signal contrast required for sing...

journal_title：Clinical chemistry

authors： Soni GV,Meller A

更新日期：2007-11-01 00:00:00

abstract：:The Center for Disease Control (CDC), the New York State Department of Health (NYSDH), the College of American Pathologists, and 23 manufacturers of diagnostic products participated in an interlaboratory study of aspartate aminotransferase (EC 2.6.1.1) methodologies. Six different lyophilized materials were prepared a...

journal_title：Clinical chemistry

authors： Burtis CA,Sampson EJ,Bayse DD,McKneally SS,Whitner VS

更新日期：1978-06-01 00:00:00

abstract：BACKGROUND:Meconium analysis is a diagnostically sensitive and objective alternative to maternal self-report for detecting prenatal tobacco exposure. Nicotine and metabolite disposition in meconium is poorly characterized, and correlation of analytes' concentrations with neonatal outcomes is unexplored. Our objectives ...

journal_title：Clinical chemistry

authors： Gray TR,Magri R,Shakleya DM,Huestis MA

更新日期：2008-12-01 00:00:00

abstract：:Market forces have dramatically influenced the environment in which healthcare is delivered, but these changes do not need to be interpreted negatively by community laboratorians. Only total vertical integration of laboratory medicine can control episode-of-care cost. Opportunities also exist for horizontal integratio...

journal_title：Clinical chemistry

authors： Forsman RW

更新日期：1996-05-01 00:00:00

abstract：:We report the adaptation of four radioassays to the prototype of an automated radioimmunoassay system ("Centria," Union Carbide). The system consists of three integrated modules: (a) an automated pipettor, which dispenses samples and reagents; (b) the key module, an incubator/separator, in which centrifugal force is u...

journal_title：Clinical chemistry

authors： Ertingshausen G,Shapiro SI,Green G,Zborowski G

更新日期：1975-08-01 00:00:00

abstract：:We describe an enzymatic fluorometric method for determining glucose concentrations in blood samples by analysis on a semi-solid surface (silicone-rubber pads). The method is an adaptation of existing spectrophotometric procedures based on the sequential coupling reactions with glucose-6-phosphate dehydrogenase and NA...

journal_title：Clinical chemistry

authors： Kiang SW,Kuan JW,Guilbault GG

更新日期：1975-11-01 00:00:00

abstract：BACKGROUND:Mutations in the retina-specific ABC transporter (ABCA4) gene have been associated with several forms of macular degenerations. Because the high complexity of the molecular genotype makes scanning of the ABCA4 gene cumbersome, we describe here the first use of denaturing HPLC (DHPLC) to screen for ABCA4 muta...

journal_title：Clinical chemistry

authors： Stenirri S,Fermo I,Battistella S,Galbiati S,Soriani N,Paroni R,Manitto MP,Martina E,Brancato R,Allikmets R,Ferrari M,Cremonesi L

更新日期：2004-08-01 00:00:00

abstract：:Intra- and interindividual components of biological variation have been determined for total thyroxin (TT4), free thyroxin (FT4), total triiodothyronine (TT3), free triiodothyronine (FT3), and thyrotropin (TSH). Calculated analytical goals (CV, %) for the precision required for optimal patient care are: TT4 less than ...

journal_title：Clinical chemistry

authors： Browning MC,Ford RP,Callaghan SJ,Fraser CG

更新日期：1986-06-01 00:00:00

abstract：:Creatine kinase (CK; EC 2.7.3.2) has been used as an indicator of myocardial cellular damage. In this study we used a Krebs-Henseleit (KH) solution to reperfuse isolated rat hearts after 24 h of cold preservation and collected the KH reperfusate for assay of CK to assess cellular damage. We wanted to determine the sta...

journal_title：Clinical chemistry

authors： Dunphy G,Ely D

更新日期：1990-05-01 00:00:00

abstract：BACKGROUND:The bleeding time (BT) test predicts a higher bleeding complication rate in populations at risk for inherited or acquired platelet dysfunction, but it is of limited assistance in evaluating individual patients. There are no reports of clinical outcomes after discontinuation of the BT test. METHODS:Interview...

journal_title：Clinical chemistry

authors： Lehman CM,Blaylock RC,Alexander DP,Rodgers GM

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:There are 45 known genetic diseases that impair the lysosomal degradation of macromolecules. The loss of a single lysosomal hydrolase leads to the accumulation of its undegraded substrates in tissues and increases of related glycoconjugates in urine, some of which can be detected by screening of free oligosa...

journal_title：Clinical chemistry

authors： Xia B,Asif G,Arthur L,Pervaiz MA,Li X,Liu R,Cummings RD,He M

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND:Endothelial nitric oxide synthase (eNOS) could be a candidate gene for coronary artery disease (CAD). This study investigated the relationship of the eNOS Glu(298)-->Asp and T(786)-->C polymorphisms with the presence and severity of CAD in the Italian population. METHODS:We enrolled 415 unrelated individual...

journal_title：Clinical chemistry

authors： Colombo MG,Paradossi U,Andreassi MG,Botto N,Manfredi S,Masetti S,Biagini A,Clerico A

更新日期：2003-03-01 00:00:00

abstract：:Reference values were determined for 23 plasma free amino acids from measurements done in 148 healthy children ranging from 0 to 18 years of age. Amino acid analysis was performed by ion-exchange chromatography. We propose a graphic form of presenting the age-specific distribution of plasma amino acid concentrations w...

journal_title：Clinical chemistry

authors： Lepage N,McDonald N,Dallaire L,Lambert M

更新日期：1997-12-01 00:00:00

abstract：:To understand the regulation of immunity one must understand the processes and the consequences of the activation of the lymphoid cell, its clonal proliferation, and the ultimate differentiation of the progeny into the effector elements that constitute the immune system. By use of polyclonal mitogens, individual T-cel...

journal_title：Clinical chemistry

authors： Kettman J,Lefkovits I

更新日期：1984-12-01 00:00:00

abstract：:I developed a standard procedure for assessing sensitivity, intra-assay precision, parallelism of sample dilutions, and assay drift, using a single trial assay kit. I used this to compare the performance of 10 commercial kits with an in-house radioimmunoassay for human thyrotropin. No one kit stood out as clearly supe...

journal_title：Clinical chemistry

authors： Evans MC

更新日期：1988-01-01 00:00:00

abstract：BACKGROUND:Nandrolone is one of the most abused anabolic steroids, and its use in doping is increasing, as revealed by numerous positive cases during recent years in various sports. Different authors have reported the possible natural production of nandrolone metabolites in humans, and some of these authors argued that...

journal_title：Clinical chemistry

authors： Baume N,Avois L,Schweizer C,Cardis C,Dvorak J,Cauderay M,Mangin P,Saugy M

更新日期：2004-02-01 00:00:00

abstract：:We developed a highly sensitive, convenient assay for measuring blocking-type anti-acetylcholine receptor antibodies, which inhibit the binding of 125I-labeled alpha-bungarotoxin (alpha-BuTx) to the acetylcholine receptor (AChR). This procedure detected inhibitory activities in sera from 76% of patients with myastheni...

journal_title：Clinical chemistry

authors： Hara H,Hayashi K,Ohta K,Itoh N,Nishitani H,Ohta M

更新日期：1993-10-01 00:00:00

abstract：:We describe a multiprobe colorimeter that permits the reading of enzyme-linked immunosorbent assays (ELISA) in situ, by use of microhemagglutination plates. The use of dipping probes eliminates interference from meniscus effects and air-bubble entrapment. Detailed validation tests have been done and potential sources ...

journal_title：Clinical chemistry

authors： McMurray CH,Blanchflower WJ

更新日期：1979-04-01 00:00:00

abstract：:Ingestion of oleander plant, containing the cardiac glycoside oleandrin, has been reported to induce fatal poisonings. Derivatives of oleandrin are structurally similar to digoxin. We investigated the cross-reactivities of oleandrin and its aglycone metabolite, oleandrigenin, in several commercially available digoxin ...

journal_title：Clinical chemistry

authors： Jortani SA,Helm RA,Valdes R Jr

更新日期：1996-10-01 00:00:00

abstract：:We measured the free amino acids in plasma of 58 patients with HIV infection and in six persons in the risk group. The HIV+ patients had significantly increased concentrations of arginine, phenylalanine, and glutamate in comparison with both age- and sex-matched controls and the members of the risk group. Glutamate co...

journal_title：Clinical chemistry

authors： Ollenschläger G,Jansen S,Schindler J,Rasokat H,Schrappe-Bächer M,Roth E

更新日期：1988-09-01 00:00:00

abstract：:Two major components of rRNA (18S and 28S rRNA) were separated by electrophoresis in injection-molded acrylic chips with a microchannel 100 microm in width, 40 microm in depth, and with 1 cm of separation distance. Microchannels were filled with 4 g/L hydroxypropylmethylcellulose as sieving polymer and 5 mg/L ethidium...

journal_title：Clinical chemistry

authors： Ogura M,Agata Y,Watanabe K,McCormick RM,Hamaguchi Y,Aso Y,Mitsuhashi M

更新日期：1998-11-01 00:00:00

abstract：BACKGROUND:The association between increases in cardiac troponin and adverse cardiac outcomes is well established. There is a growing interest in exploring routine cardiac troponin monitoring as a potential early indicator of adverse heart health trends. Prognostic use of cardiac troponin measurements requires an assay...

journal_title：Clinical chemistry

authors： Jarolim P,Patel PP,Conrad MJ,Chang L,Melenovsky V,Wilson DH

更新日期：2015-10-01 00:00:00

abstract：:We investigated glycosylated hemoglobin S by means of chromatography on Bio-Rex 70. The selected elution conditions were similar to those described by Trivelli et al. (N Engl J Med 284: 353-357, 1971), except for modified ionic strength and accurate temperature control. This enabled us to isolate a minor hemoglobin fr...

journal_title：Clinical chemistry

authors： Cirotto C,Allegrucci M,Cartechini MG,Compagnucci P,Santeusanio F,Bolli G

更新日期：1985-04-01 00:00:00

abstract：BACKGROUND:Clinical proteomics presents great promise in biology and medicine because of its potential for improving our understanding of diseases at the molecular level and for detecting disease-related biomarkers for diagnosis, prognosis, and prediction of therapeutic responses. To realize its full potential to impro...

journal_title：Clinical chemistry

authors： Boja ES,Jortani SA,Ritchie J,Hoofnagle AN,Težak Ž,Mansfield E,Keller P,Rivers RC,Rahbar A,Anderson NL,Srinivas P,Rodriguez H

更新日期：2011-04-01 00:00:00

abstract：BACKGROUND:We evaluated the analytical performance of 4 cystatin C assays (Siemens N Latex on BNII, Roche Tina-quant on Cobas c501, Genzyme on Cobas c501, and Tosoh ST AIA-PACK on Tosoh AIA-600II) according to guidelines published by the Clinical and Laboratory Standards Institute. METHODS:We evaluated total imprecisi...

journal_title：Clinical chemistry

authors： Li J,Dunn W,Breaud A,Elliott D,Sokoll LJ,Clarke W

更新日期：2010-08-01 00:00:00

abstract：BACKGROUND:Standardization of clinical measurements of C-reactive protein (CRP) is based on the availability of reference materials. The serum protein reference material ERM-DA470 was used as a calibrant for various commercially available immunoassays but has now been exhausted. The recently released ERM-DA470k/IFCC wa...

journal_title：Clinical chemistry

authors： Rzychon M,Zegers I,Schimmel H

更新日期：2010-09-01 00:00:00

abstract：BACKGROUND:Two mutations in HFE, G845A (amino acid substitution C282Y) and C187G (H63D), are associated with hereditary hemochromatosis. We developed and validated a novel method, linked linear amplification (LLA), for detection of these two mutations. METHODS:Two segments of HFE were amplified by a multiplex LLA reac...

journal_title：Clinical chemistry

authors： Killeen AA,Breneman JW 3rd,Carillo AR,Ugozzoli LA,Lowery JD,Liu J,Hixson CS

更新日期：2003-07-01 00:00:00