Abstract：
:The aim of this study was to verify whether prolonged exposure of cultured rat anterior pituitary cells to high glucose can alter growth hormone (GH) release and responsiveness to secretagogues. Therefore, we cultured anterior pituitary cells obtained from normal male Sprague-Dawley rats in presence of normal (6 mM) or high (22 mM) glucose concentrations. After 3 days, the acute effects of glucose, growth hormone-releasing factor (GRF), dibutyryl cyclic AMP(db-cAMP) and somatostatin were studied during 2-hour incubations. High glucose did not alter basal GH release from cells cultured in 6 mM glucose. However, basal GH release from cells cultured in 22 mM glucose was moderately higher in the 2-hour incubation (by 46%) than in cells cultured in 6 mM glucose. In contrast, GH stimulation by GRF or db-cAMP was significantly reduced in cells cultured in 22 mM as compared to cells cultured in 6 mM glucose. This inhibitory effect of high glucose on GRF-stimulated GH release was completely reversible after 24 h of exposure of the cultured cells to 6 mM glucose and testing on the 4th day of culture. Finally, GH inhibition by somatostatin was also attenuated in cells cultured with high glucose. We conclude that prolonged exposure to high glucose could act directly at the pituitary level to modulate GH release and responsiveness.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Renier G,Serri Odoi
10.1159/000125947subject
Has Abstractpub_date
1991-11-01 00:00:00pages
521-5issue
5eissn
0028-3835issn
1423-0194journal_volume
54pub_type
杂志文章abstract：:The effects of GABAa receptor activation on the secretion of alpha-melanocyte-stimulating hormone (alpha-MSH) from the superfused pars intermedia of the amphibian Xenopus laevis were examined. The GABAa receptor agonist isoguvacine inhibited secretion of alpha-MSH from the pars intermedia, an action completely antagon...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126516
更新日期：1993-07-01 00:00:00
abstract：:Estradiol rapidly regulates the activity of arcuate nucleus (ARH) proopiomelanocortin (POMC) neurons that project to the medial preoptic nucleus (MPN) to regulate lordosis. Orphanin FQ/nociceptin (OFQ/N) acts via opioid receptor-like (ORL)-1 receptors to inhibit these POMC neurons. Therefore, we tested the hypothesis ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000443765
更新日期：2016-01-01 00:00:00
abstract：:The postnatal development of the luteinizing hormone-releasing hormone (LHRH) system in male and female rats was investigated using light microscopic immunocytochemistry. An identical number of immunoreactive LHRH cells (about 1,300) was observed for males and females throughout development. Two LHRH cell types, based...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124516
更新日期：1986-01-01 00:00:00
abstract：:Sexual behavior and elevation of gonadal steroids were induced in both male and female New Zealand white rabbits following injection of synthetic beta (1-24) ACTH into the lateral cerebral ventricle. In blood assays collected by cardiac puncture, testosterone, progesterone and corticosterone were all increased above b...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122441
更新日期：1975-01-01 00:00:00
abstract：:The hypophysial portal vessels and anterior pituitary glands of adult male rats were surgically exposed, cannulated and infused for 1 min with saline, growth hormone-releasing factor (GH-RF), and dbcAMP. After cessation of infusion, anterior pituitary glands were collected at 1, 5, 15, 30 or 60 min for electron micros...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122421
更新日期：1975-01-01 00:00:00
abstract：:The frequency of pulsatile release of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) is high in the breeding season and low in the nonbreeding season. These alterations in the patterns of GnRH and LH release are due to an interaction of daylength and gonadal steroid negative feedback. A vast amount...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000076631
更新日期：2004-02-01 00:00:00
abstract：:In human African trypanosomiasis (sleeping sickness), sleep and wake episodes are sporadically distributed throughout the day and the night. Plasma melatonin, sleep-wakefulness and rectal temperature rhythms were studied in 9 Congolese patients suffering from sleeping sickness compared to 6 healthy controls submitted ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054351
更新日期：1998-07-01 00:00:00
abstract：:Concentrations of luteinizing hormone (LH) and prolactin (Prl) in the serum of anestrous and cycling ewes were compared. The concentration of LH in serum and the frequency of episodic releases of LH were lower in anestrous than in cycling ewes. Concentrations of LH in serum rose as rapidly after ovariectomy of anestro...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122870
更新日期：1979-01-01 00:00:00
abstract：:In the present study the effects of intravenously administered corticotropin-releasing hormone (CRH) on the release of proopiomelanocortin (POMC) derivatives such as adrenocorticotropic hormone (ACTH), beta-lipotropin (beta-LPH) and beta-endorphin (beta-END) as well as direct effects of CRH on pain sensitivity were ex...
journal_title：Neuroendocrinology
pub_type： 杂志文章,随机对照试验
doi：10.1159/000091980
更新日期：2005-01-01 00:00:00
abstract：:Light deprivation by blinding or exposure to short photoperiod results in depressed prolactin (PRL) cell activity and gonadal regression in the male Syrian hamster after at least 42-56 days of treatment, and both of these events can be prevented if pineal removal accompanies light deprivation. In the present study, we...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125272
更新日期：1989-11-01 00:00:00
abstract：:Multiple unit activity (MUA) was recorded from the adrenocorticotropic area (n. posterior medialis hypothalami, PMH) of unrestrained resting pigeons throughout the 24 h period and compared with plasma corticosterone levels (B). Bilateral electrolytic lesions of the hippocampus suppressed diurnal variations of MUA and ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122750
更新日期：1978-01-01 00:00:00
abstract：:A significant increase of cell multiplication in inoculated ascitic and solid tumors was demonstrated in both DBA/2 and C57BL/6 mice as well as in Wistar rats after radiofrequency lesions in the median hypothalamus (ventromedial and dorsomedial nuclei; part of arcuate nucleus). The following tests were performed: mito...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124479
更新日期：1986-01-01 00:00:00
abstract：:Brain sexual differentiation is mediated through testosterone, which acts during the perinatal period in the form of both 5alpha-dihydrotestosterone and estradiol. In order to gain insight into the molecular mechanisms involved, we studied induction of c-fos, an index of functional neuronal activation, in the 2-day-ol...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054657
更新日期：2001-06-01 00:00:00
abstract：:The arcuate nucleus-median eminence complex (AM) undergoes major structural and functional changes during normal puberty or if exposed to a pulse of estradiol in the prepuberal period. Those changes are expressed by increased synaptogenesis and by a drastic alteration in the feedback control of anterior pituitary glan...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124775
更新日期：1987-06-01 00:00:00
abstract：:Steroid autoradiography was undertaken to determine the neuroanatomical loci which might be involved in the activation of steroid-sensitive behaviors in the Japanese quail (Coturnix japonica). Male and female quail were either surgically gonadectomized or photically regressed and implanted with androgen or estrogen to...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125091
更新日期：1989-01-01 00:00:00
abstract：:Observations of long standing have suggested that the 'stress' of chair restraint inhibits the GnRH pulse generator in normal female monkeys while this phenomenon is rarely observed in ovariectomized animals. The role of the ovary in the response of the GnRH pulse generator to the stress of insulin hypoglycemia was in...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126291
更新日期：1992-11-01 00:00:00
abstract：:High affinity binding of 3H-flunitrazepam (FNZP) to crude membrane preparations of bovine pineal membranes was examined by a rapid filtration procedure through Whatman GFB paper. At 0 degrees C binding reached equilibrium in about 20 min. Scatchard analysis of data at equilibrium revealed a single population of bindin...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123533
更新日期：1983-08-01 00:00:00
abstract：:Structural and functional impairment of the skeletal system remains an important cause of morbidity and disability in patients with Cushing's syndrome (CS). Glucocorticoid (GC) excess inhibits bone formation and calcium absorption from the gut, increases bone resorption, and alters the secretion of gonadotropin and gr...
journal_title：Neuroendocrinology
pub_type： 杂志文章,评审
doi：10.1159/000314298
更新日期：2010-01-01 00:00:00
abstract：:This study compares the processing of pro-opiomelanocortin (POMC) at two Lys-Lys cleavage sites, located in the carboxy-terminal domain of the precursor, one site marking the amino terminus of beta-melanocyte-stimulating hormone (beta-MSH) and the other in the carboxy-terminus of beta-endorphin (beta E). These compari...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126518
更新日期：1993-07-01 00:00:00
abstract：:Interactions between the hypothalamic-pituitary-adrenocortical (HPA) system and melatonin secretion have been demonstrated, but only the effects of melatonin on the activity of the HPA system have been studied in man. Alterations of melatonin secretion described as low-melatonin syndrome have been demonstrated in pati...
journal_title：Neuroendocrinology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1159/000127186
更新日期：1997-04-01 00:00:00
abstract：:The modulatory actions of ovarian steroids on the norepinephrine (NE) induced alterations in luteinizing hormone (LH) secretion were examined in male and female rats. Long-term (4 weeks) castrated male and female rats bearing chronic third-ventricle cannulae were implanted with intra-atrial catheters. Animals were ble...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124581
更新日期：1986-01-01 00:00:00
abstract：:Intracranial implants of estradiol benzoate (EB) were used to explore sites of action for the activation of masculine copulatory behavior in castrated male rats. In experiment 1, 27 or 30 gauge implants were placed unilaterally throughout the hypothalamus and preoptic area. Dihydrotestosterone (DHT) was administered s...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122865
更新日期：1979-01-01 00:00:00
abstract：:In pituitary cells, c-fos transcription induced by releasing hormones and growth factors results from enhanced initiation of transcription, and sustained elongation of transcripts beyond the first intron. We studied the regulatory role of the first intron of the mouse c-fos gene for the control of its transcription in...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054606
更新日期：2000-12-01 00:00:00
abstract：:The present study was aimed at evaluating chronic stress models in mice with special attention to morphine treatment. We hypothesized that repeated periods of drug withdrawal induce chronic stress. To verify this hypothesis, mice were made dependent on morphine and then subjected to several types of repeated withdrawa...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000087034
更新日期：2005-01-01 00:00:00
abstract：:The timing of the episodic secretion of luteinizing hormone (LH) in ovariectomized ewes was investigated with respect to an ultradian rest-activity cycle. Individual sheep were enclosed in a chamber where their physical activity was continuously monitored, and blood samples for LH analysis were collected at 5-min inte...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123187
更新日期：1981-06-01 00:00:00
abstract：:The acute effects of hypothalamic electrical stimulation on the secretion of FSH and LH have been examined in anestrous and estrous ferrets. Basal levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were higher in the anestrous than in the estrous animals; the anestrous animals showed a more mark...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122674
更新日期：1977-01-01 00:00:00
abstract：:The aim of this study was to examine the occurrence of endogenous oscillations of Per1, Per2, Bmal1 and Rev-erbα genes in rat pineal explants and to investigate their regulation by adrenergic ligands. Our results show a significant and sustained rhythm of Per2,Bmal1 and Rev-erbα gene expression for up to 48 h in cultu...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000327430
更新日期：2011-01-01 00:00:00
abstract：:The influence of catecholamines (CA) on hypothalamic somatostatin (HPT-SRIF) was investigated in rats by using several drugs which interfere with brain CA metabolism. Depletion of brain CA stores by alpha-methyl-rho-tyrosine (AMT) increased HPT-SRIF, while augmented brain CA levels following L-dopa administration decr...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123374
更新日期：1982-01-01 00:00:00
abstract：:The influence of the hypothalamus on the development of the anterior pituitary cells was studied in normal and anencaphalic human fetuses. An immunocytological method was applied to study the quantitative evolution of the size of the cells during gestation. The somatotropic and corticotropic cells appeared at the end ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122709
更新日期：1977-01-01 00:00:00
abstract：:A number of neurochemical messengers have been shown to act directly on the melanotrope cells of the pars intermedia of Xenopus laevis to regulate alpha MSH secretion. In the present study the possibility that the melanotropes are also indirectly controlled has been examined. For this purpose, the characteristics of a...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125853
更新日期：1991-07-01 00:00:00
