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Surface charge of purple membranes measured by laser Doppler velocimetry.

Abstract：

:Laser Doppler velocimetry measurements on purple membrane suspensions from Halobacterium halobium showed a linear correlation between electrophoretic mobility and applied electric field, electrokinetic responses could be rapidly monitored. Native membranes are less charged than white membrane preparations (from the R1mW mutant). Chemical modification of carboxyl residues reduces surface charge, and nitrotyrosine modified membranes are more or less charged than native membranes at pH greater than or less than 6.5, respectively. Changes in surface charge are found upon actinic illumination and are greatest (Ca 5 X 10(-4)/A2) under conditions where decay of the M412 intermediate of the photoreaction cycle is inhibited, such as at high pH or after chemical modification.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Packer L,Arrio B,Johannin G,Volfin P

doi

10.1016/0006-291x(84)90467-4

subject

Has Abstract

pub_date

1984-07-18 00:00:00

pages

252-8

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(84)90467-4

journal_volume

122

pub_type

杂志文章

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