Donor-reactive cytokine production after HLA-identical living related kidney transplantation: a protein-array analysis.

Abstract:

:In the present pilot study, we investigated which proteins are produced after donor stimulation of peripheral blood mononuclear cells from recipients of HLA-identical living related kidney transplant. We used a protein-array analysis to determine cytokines, chemokines, and growth factors in supernatant from donor-stimulated mixed lymphocyte reaction cultures. Autologous cultures were considered to be negative controls. In 38 out of 42 proteins (90%), the donor response was higher compared with the autologous response. Therefore, we concluded that even after HLA-identical living related kidney transplantation we could measure a donor-reactive response, which we assumed was directed toward minor histocompatibility or non-HLA antigens.

journal_name

Transplant Proc

authors

Gerrits JH,van de Wetering J,Weimar W,van Besouw NM

doi

10.1016/j.transproceed.2006.08.183

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

2825-7

issue

9

eissn

0041-1345

issn

1873-2623

pii

S0041-1345(06)01126-2

journal_volume

38

pub_type

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