Abstract:
:Acyclovir and its major impurity guanine are determined rapidly by the incorporation of a monolithic column (100 mm x 4.6 mm i.d., Merck) to an automated HPLC system. A simple flow gradient protocol was adopted in order to accelerate the separation-detection cycle. Using 0.2% CH(3)COOH (pH 3.1) as the mobile phase and detection at 254 nm, guanine was effectively separated from the system peak (t(R)=1.25 min), while the retention time of acyclovir was 2.35 min. Linearity of the assay was validated in the range 0.1-1.0% guanine and 80-120% acyclovir (n=5). The accuracy and within- and day-to-day precision of the method were also validated, while the limits of detection and quantitation of both analytes were determined. The proposed method was successfully applied to the quality control of acyclovir raw material and the quality and stability control of acyclovir-containing pharmaceutical creams (Hagevir 5%, w/w, Cosmopharm Ltd., Korinthos, Greece).
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Tzanavaras PD,Themelis DGdoi
10.1016/j.jpba.2006.11.002subject
Has Abstractpub_date
2007-03-12 00:00:00pages
1526-30issue
4eissn
0731-7085issn
1873-264Xpii
S0731-7085(06)00736-9journal_volume
43pub_type
杂志文章abstract::Capillary electrophoresis (CE) methods for transferrin analysis are widely used in clinical laboratories, but complement C3 peaks often overlap with carbohydrate-deficient transferrin peaks. In this study, we discovered that the electrophoretic mobility of the complement C3 peak could be controlled by adding carboxyme...
journal_title:Journal of pharmaceutical and biomedical analysis
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abstract::A micellar electrokinetic chromatographic (MEKC) method was developed for the quantification of lovastatin and simvastatin, cholesterol lowering agents in pharmaceutical dosage forms. Lovastatin and simvastatin were separated using an electrolyte system consisting of 12% acetonitrile (v/v) in 25 mM sodium borate buffe...
journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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abstract::Co-crystallization brings new opportunities for improving the solubility and dissolution rate of drugs with the chance of finely tuning some relevant chemical-physical properties of mixtures containing bioactive compounds. As co-crystallization process involves several molecular species, which are generally solid at r...
journal_title:Journal of pharmaceutical and biomedical analysis
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更新日期:2013-05-05 00:00:00
abstract::Understanding the origin and fate of organic impurities within the manufacturing process along with a good control strategy is an integral part of the quality control of drug substance. Following the underlying principles of quality by design (QbD), a systematic approach to analytical control of process impurities by ...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2010-08-01 00:00:00
abstract::Capillary electromigration techniques have become important tools for the analysis of pharmaceutical drugs. The present review updates on recent developments of the analysis of small molecule drugs, excipients and counter ions dating between January 2014 and May 2017. Capillary electrophoresis (CE) continues to be a v...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章,评审
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journal_title:Journal of pharmaceutical and biomedical analysis
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abstract::A new, rapid, simple and specific method to determine 5-chloro 8-hydroxyquinoline (5-HQ) and 5,7-dichloro 8-hydroxyquinoline (5,7-HQ) stability in swine feed was optimized and validated. A system consisting of an ACQUITY UPLC BEH C18 column (1.7 μm, 2.1 mm × 100 mm), a mobile phase of acetonitrile-0.1% o-phosphoric ac...
journal_title:Journal of pharmaceutical and biomedical analysis
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更新日期:2019-03-20 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2016-11-30 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2008-03-13 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2013-05-05 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2012-03-25 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2011.05.025
更新日期:2011-09-10 00:00:00
abstract::A reversed-phase high performance liquid chromatographic (LC), tandem mass spectrometry (MS/MS) assay for the determination of tenofovir (TFV) and emtricitabine (FTC) in dried blood spots (DBS) from human whole blood was developed and validated. Whole blood samples were spotted, dried, and a 3mm punch was extracted wi...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2013.08.033
更新日期:2014-01-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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abstract::A rapid technique for sample preparation and HPLC was developed to study in vitro the erythrocyte binding and plasma protein binding of fleroxacin in the concentration range 0.2-15 micrograms ml-1. The red blood cell partition coefficient of fleroxacin was 1.45 +/- 0.18 at 25 degrees C and was independent of concentra...
journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章,评审
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2015-03-15 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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abstract::A simple and specific high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) absorbance detection has been developed for the determination of isoferulic acid in rat plasma. The plasma samples were deproteinized with methanol after the addition of internal standard (IS) tinidazole. The analysis was...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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