Abstract:
:An improved high performance liquid chromatographic assay for plasma-unbound atenolol is described. The assay has a wide range (10-5000 ng ml-1) of linearity and a detection limit of 5 ng ml-1 (or 0.1 ng per injection) with acceptable intra- and inter-assay reproducibilities using small volumes of plasma (100 microliters). Following administration of a single dose of atenolol to the rat, nine blood samples were collected over a period of 8 h. These samples were analyzed for atenolol concentrations by a sensitive and specific microbore high performance liquid chromatograph with a photodiode-array detector. This multi-channel detector was used to acquire spectral information on atenolol and demonstrated a superior performance in comparison to all other techniques in that both qualitative and quantitative information were acquired with the system. Because of it sensitivity and applicability to plasma analysis, the assay can be used for pharmacokinetic studies and is valuable in therapeutic drug monitoring.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Cheng FC,Chen YT,Kuo JS,Chen SH,Chang LCdoi
10.1016/s0731-7085(96)01760-8subject
Has Abstractpub_date
1996-06-01 00:00:00pages
1169-74issue
8-10eissn
0731-7085issn
1873-264Xpii
S0731-7085(96)01760-8journal_volume
14pub_type
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journal_title:Journal of pharmaceutical and biomedical analysis
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