Abstract:
:Bovine granulosa cells seeded in the presence of serum on extracellular matrix-coated dishes proliferate actively when exposed to serum-free medium supplemented with insulin (2 microgram/ml), fibroblast growth factor (FGF, 100 ng/ml), and high density lipoprotein (HDL, 30 microgram protein/ml). The final density of the cultures is 80-120% that of cultures grown in the presence of medium supplemented with optimal concentration (10%) of calf serum. Insulin has the greatest effect on cell proliferation when added alone to serum-free medium, since it induced an increase in cell number that was 35-60% that observed with optimal serum concentration. Somatomedin C can replace insulin when added alone. FGF, epidermal growth factor, or HDL had no significant effect on cell proliferation by themselves. When these factors were added together with insulin, they acted synergistically in stimulating cell proliferation. When cultures were seeded in the total absence of serum, the addition of transferrin (10 microgram/ml) to serum-free medium was required in order for insulin and FGF to be mitogenic. Cultures maintained on extracellular matrix and exposed to serum-free medium alone have a lifespan in culture of 4 generations. Addition of insulin, FGF, and HDL increases the lifespan of the cultures to 12 generations. Bovine granulosa cells, which proliferate in a defined medium, respond to dibutyryl cAMP by releasing progesterone into the medium. Addition of FSH to the defined medium resulted in a 30% decrease in cell proliferation and in a 2.1-fold increase in the amount of progesterone released into the medium in response to dibutyryl cAMP. This release of progesterone reached a level similar to that observed with cultures grown in medium supplemented with optimal concentration of serum and exposed or not to FSH during their growth phase and at confluence. These results demonstrate that bovine granulosa cells can actively proliferate in a serum-free medium and maintain their differentiated function, as indicated by their ability to produce progesterone.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Savion N,Lui GM,Laherty R,Gospodarowicz Ddoi
10.1210/endo-109-2-409subject
Has Abstractpub_date
1981-08-01 00:00:00pages
409-20issue
2eissn
0013-7227issn
1945-7170journal_volume
109pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::2,5-Hexanedione (2,5-HD) exposure in the rat produces irreversible testicular atrophy, a model of human male infertility that can be used for mechanistic and therapeutic studies. Following testicular injury by 2,5-HD, stem cell factor (SCF), a Sertoli cell-derived growth factor that binds the c-kit receptor on spermat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5678
更新日期:1998-01-01 00:00:00
abstract::Cultured pulmonary artery endothelial (CPAE) cells possess specific high affinity receptors for atrial natriuretic peptide (ANP). CPAE cells were used to investigate the regulation of ANP receptors by glucocorticoids. Treatment of CPAE cells with dexamethasone (1 microM) produced a 69.3 +/- 23% (P less than 0.01) incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-5-2311
更新日期:1991-11-01 00:00:00
abstract::Methylamine and monensin are two agents known to interfere with the recycling of membrane receptors. In the present investigation, we studied their effects on the binding, intracellular distribution, and action of insulin in isolated pancreatic acini prepared from diabetic mice. These drugs had several similar effects...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-205
更新日期:1984-07-01 00:00:00
abstract::PTH is an important peptide hormone regulator of calcium homeostasis and osteoblast function. However, its mechanism of action in osteoblasts is poorly understood. Our previous study demonstrated that PTH activates mouse osteocalcin (Ocn) gene 2 promoter through the osteoblast-specific element 1 site, a recently ident...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1573
更新日期:2008-04-01 00:00:00
abstract::We studied expression of the c-myc and c-ras protooncogenes during the initiation of pig thyroid cells in primary cell culture. This period is associated with major changes in differentiated thyroid cell function. After 1, 2, and 3 days of culture, polyadenylated mRNA was prepared from 30-50 replicate dishes of cells....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-3-924
更新日期:1987-03-01 00:00:00
abstract::In mammals, the brain-specific protein munc18-1 regulates synaptic vesicle exocytosis at the synaptic junction, in a step before vesicle fusion. We hypothesize that the rate of biosynthesis of munc18-1 messenger RNA (mRNA) and the amount of munc18-1 present in neurons and neuroendocrine cells are related to the physio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.5.8131
更新日期:2001-05-01 00:00:00
abstract::Uterine epithelial cells (UEC) isolated from 6-week-old CF-1 mice were immortalized using retroviral-mediated transfection of SV40 large T-antigen. One line, WEG-1, retained epithelial morphology and reacted with antibodies to cytokeratins 18, 19, laminin, fibronectin, and beta-catenin. In addition, WEG-1 cells displa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536610
更新日期:1996-01-01 00:00:00
abstract::The effects of epidermal growth factor (EGF) on glycogen metabolism and the binding of [125I]iodo-EGF to receptors in fetal rat hepatocytes have been examined. The actions of EGF have been compared with those of insulin-like growth factor I (IGF-I) and insulin. EGF (0.1-45 nM) stimulated dose-dependent increases in [1...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-522
更新日期:1986-08-01 00:00:00
abstract::We have studied regulation of the glucose transporter by thyroid hormone in ARL 15 cells, a thyroid hormone-responsive cell line derived from rat liver, T3 treatment (5 x 10(-8) M for 48 h) of confluent cell monolayers grown in thyroid hormone-deficient medium increased the rate of uptake of [3H] 2-deoxyglucose by 2.3...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1421
更新日期:1990-03-01 00:00:00
abstract::During the third week of postnatal life, dramatic ontogenic changes occur in the morphology and enzymology of the small intestine of the infant rat, enabling the animal to make the transition from milk to solid food. To investigate the roles of T4 and GH in regulation of these changes, infant rats were hypophysectomiz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-3-1417
更新日期:1991-09-01 00:00:00
abstract::MRL-lpr/lpr mice manifest a systemic lupus-like autoimmune disease. As part of this syndrome, the mice spontaneously develop autoimmune thyroiditis, which is morphologically and biochemically similar to human autoimmune thyroiditis. In this study we investigated whether thyroid tissue obtained from sites of chronic in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628400
更新日期:1995-08-01 00:00:00
abstract::Atrial, brain-type, and C-type natriuretic peptides (ANP, BNP, and CNP) act via receptors with intrinsic guanylate cyclase activity. The A-type and B-type ANP receptors are selectively activated by ANP and CNP, respectively. The anterior pituitary is a site of ANP production and action, suggesting a local regulatory f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.5.7682940
更新日期:1993-05-01 00:00:00
abstract::Mutations of the monocarboxylate transporter 8 gene (MCT8, SLC16A2) cause the Allan-Herndon-Dudley syndrome, an X-linked syndrome of severe intellectual deficit and neurological impairment. Mct8 transports thyroid hormones (T4 and T3), and the Allan-Herndon-Dudley syndrome is likely caused by lack of T3 transport to n...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1014
更新日期:2011-04-01 00:00:00
abstract::The maternal skeleton resorbs during lactation to provide calcium to milk and the lost mineral content is restored after weaning. The changes are particularly marked in Ctcgrp null mice, which lose 50% of spine mineral content during lactation but restore it fully. The known calciotropic hormones are not required for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1931
更新日期:2013-04-01 00:00:00
abstract::The tissue-specific differential processing of proglucagon (Pg) yields glucagon in pancreatic A cells and glucagon-like peptide-1 (GLP-1), GLP-2, and glicentin in intestinal L cells. It has been suggested that the difference in Pg cleavage in A and L cells is due to the presence of distinct prohormone convertases (PC)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7068
更新日期:1999-10-01 00:00:00
abstract::The nuclear orphan receptor human estrogen receptor-related receptor (ERR)-alpha is implicated in bone metabolism. We studied the effect of ERRalpha silencing in human mesenchymal stem cells (hMSCs) during osteoblastogenesis. We found that ERRalpha silencing led to an increase of bone sialoprotein and a decrease of os...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0121
更新日期:2009-10-01 00:00:00
abstract::GH is a pleiotropic hormone that plays a major role in proliferation, differentiation, and metabolism via its specific receptor. It has been previously suggested that GH signaling pathways are required for normal liver regeneration but the molecular mechanisms involved have yet to be determined. The aim of this study ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1193
更新日期:2011-07-01 00:00:00
abstract::The mechanism of the inhibitory effect of long term treatment with estradiol on dopamine synthesis in tuberoinfundibular dopaminergic (TIDA) neurons was studied by using hypothalamic slices from ovariectomized rats. Treatment with 2 mg estradiol valerate (EV) at a 3-week interval increased the weight of the anterior p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-2-692
更新日期:1987-08-01 00:00:00
abstract::The induction of hepatic drug metabolizing enzymes alters not only the metabolism of the xenobiotic substances that induce them but also the metabolism of various endogenous hormones. The xenobiotic receptor constitutive androstane receptor (CAR) (NR1I3) mediates the well-studied induction of CYP2B genes and other dru...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1350
更新日期:2005-03-01 00:00:00
abstract::Inflammation can interfere with endometrial receptivity. We examined how interleukin 1β (IL-1β) affects expression of the uterine gap junction protein connexin 43 (Cx43), which is known to be critical for embryonic implantation. We used an in vitro model of human endometrial stromal cells (ESCs), Western blotting, and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00495
更新日期:2017-12-01 00:00:00
abstract::The effects of leptin on the levels of CRF messenger RNA (mRNA) in the paraventricular hypothalamic nucleus (PVN), on the activation of the PVN CRF cells, and on the plasma levels of corticosterone were investigated in lean (+/?) and obese (ob/ob) C57BL/6J male mice. Murine leptin was s.c. infused using osmotic minipu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5889
更新日期:1998-04-01 00:00:00
abstract::The family of CRH-related peptides are suggested to play important roles in the control of myometrial contractility during pregnancy and labor. In this study we investigated the expression of urocortin II (UCN II) in human myometrium and its ability to phosphorylate intracellular components that can be involved in mod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1210
更新日期:2004-02-01 00:00:00
abstract::The androgen receptor (AR) from calf uterine cytosol has been studied in terms of steroid-binding affinity, hormone dissociation kinetics, and DNA-cellulose-binding capacity. The binding affinity for three androgens, analyzed under conditions where binding to progesterone receptor did not occur, decreased in the order...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-851
更新日期:1986-02-01 00:00:00
abstract::Women with polycystic ovary syndrome (PCOS) and hyperandrogenism have altered hormone levels and suffer from ovarian dysfunction leading to subfertility. We have attempted to generate a model of hyperandrogenism by feeding mice chow supplemented with dehydroepiandrosterone (DHEA), an androgen precursor that is often e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2140
更新日期:2013-12-01 00:00:00
abstract::Puberty in primates is timed by 2 hypothalamic events: during late infancy a decline in pulsatile GnRH release occurs, leading to a hypogonadotropic state that maintains quiescence of the prepubertal gonad; and in late juvenile development, pulsatile GnRH release is reactivated and puberty initiated, a phase of develo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2154
更新日期:2013-05-01 00:00:00
abstract::In the present study, we have investigated the cellular mechanisms of androgen-induced antral follicular growth arrest and the possible involvement of chemerin and its receptor chemokine-like receptor 1 (CMKLR1) in this process, using a chronically androgenized rat model. We hypothesize that hyperandrogenism induces a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1001
更新日期:2013-08-01 00:00:00
abstract::With the rapid changes in lifestyle in modern society, including the high nutritional intake and reduced physical activity, the incidence of metabolic diseases has been increasing year by year. Obstructive sleep apnea syndrome (OSAS) is a sleep disorder, usually characterized by sudden pauses of breathing during sleep...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2018-00248
更新日期:2018-07-01 00:00:00
abstract::Thyroid hormone (TH) is required for frog metamorphosis, and corticosterone (CORT) increases TH signaling to accelerate metamorphic progression. However, a requirement for CORT in metamorphosis has been difficult to assess prior to the recent development of gene-editing technologies. We addressed this long-standing qu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa193
更新日期:2020-12-01 00:00:00
abstract::Interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) are proinflammatory cytokines that affect the secretion of several neuroendocrine hormones. In addition, glial cells synthesize and release IL-6, suggesting a paracrine role for this cytokine in the brain. We have examined the regulation of IL-6 release from glial ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6536
更新日期:1999-02-01 00:00:00
abstract::Although the neurohypophyseal hormone oxytocin (OT) is best know for its role in reproduction, OT also stimulates natriuresis at physiological plasma levels. This effect is mediated via specific renal OT receptors (OTRs). In the present study, we have characterized rat renal OTR gene transcripts and assessed their reg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.7.8770890
更新日期:1996-07-01 00:00:00